【摘要】：目的建立生活飲用水中安賽蜜、三氯蔗糖、阿斯巴甜、糖精鈉、甜蜜素5種人工合成甜味劑的固相萃取-液相色譜-質(zhì)譜(HPLC-MS-MS)測定法。方法采用HLB固相萃取(SPE)柱對生活飲用水中人工合成甜味劑進行提取和富集后,以ACQUITY UPLC HSS T3色譜柱(100 mm×2.1 mm,1.7μm)為分析柱,以乙腈-10 mmol/L乙酸銨(含2 mmol/L Tris)為流動相進行梯度洗脫,以負(fù)離子噴霧模式電離,多反應(yīng)離子監(jiān)測方式進行定性及定量檢測。結(jié)果在5~200μg/L的線性范圍內(nèi),5種人工合成甜味劑的回歸方程均呈較好的線性關(guān)系,r0.999 6。該方法的檢出限為0.2~8.0 ng/L,平均回收率為82.1%~96.3%,RSD為3.9%~9.0%。結(jié)論該方法操作簡單、快捷,靈敏度和準(zhǔn)確度均較高,精密度較好,適用于生活飲用水中5中人工合成甜味劑的測定。
[Abstract]:Objective to establish a solid phase extraction-liquid chromatography-mass spectrometry (HPLC-MS-MS) method for the determination of 5 synthetic sweeteners, such as aspartame, saccharin, aspartame, sodium and cyclamate in drinking water. Methods the synthetic sweeteners in drinking water were extracted and enriched by HLB solid phase extraction (SPE) column. The chromatographic column of ACQUITY UPLC HSS T3 (100mm 脳 2.1mm ~ 1.7 渭 m) was used as analytical column, and the mobile phase of acetonitrile-10 mmol/L ammonium acetate (including 2 mmol/L Tris) was used for gradient elution. Negative ion spray mode ionization and multi-reaction ion monitoring were used for qualitative and quantitative detection. Results within the linear range of 5 渭 g / L, the regression equations of 5 synthetic sweeteners showed a good linear relationship (r 0.999 6). The detection limit of this method is 0.2ng / L, and the average recovery is 82.1 and 96.3RSD = 3.9ng / L, respectively. Conclusion the method is simple, rapid, sensitive and accurate, and is suitable for the determination of synthetic sweeteners in drinking water.
【作者單位】： 天津市疾病預(yù)防控制中心理化室;
【分類號】：O657.63;R123.1

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