背景肝細(xì)胞受損可增加糖尿�。―iabetes mellituspatients,DM）患者發(fā)病率和死亡率。已公認(rèn),高血糖是DM患者肝損害發(fā)病的主要原因,與DM相關(guān)的肝細(xì)胞損害是DM患者常見的并發(fā)癥。磷酸肌酸（Phosphocreatine,PCr）分子作為一種可迅速動(dòng)員、回收ATP,對(duì)腦和骨骼肌的能量?jī)?chǔ)備,可作為細(xì)胞能量?jī)?chǔ)備形式,它具有多種活性。然而,PCr是否具有保護(hù)DM相關(guān)肝損傷的作用尚未見報(bào)道。本研究旨在評(píng)價(jià)PCr是否能保護(hù)DM并發(fā)的肝損傷,并探討其在體內(nèi)外作用機(jī)制。材料與方法體外研究:丙酮醛（methylglyoxal,MGO）,是葡萄糖的活性代謝產(chǎn)物,與高血糖可引起DM相關(guān)的肝細(xì)胞損傷,且與肝細(xì)胞損害進(jìn)展關(guān)系密切。Hep G2細(xì)胞培養(yǎng)及細(xì)胞進(jìn)行預(yù)處理MGO兩小時(shí),然后,分別用不同濃度的PCR處理細(xì)胞（5m M,10m M,20m M）。采用MTT法研究胞形態(tài)學(xué)、細(xì)胞毒性、細(xì)胞凋亡;流式細(xì)胞儀Annexin V-FITC、Calcien法分別檢測(cè)細(xì)胞凋亡、增殖試驗(yàn)。通過(guò)Western blotting檢測(cè)凋亡蛋白Bax、Bcl-2和Caspase-3、Caspase-9蛋白表達(dá)... 

【文章來(lái)源】：大連醫(yī)科大學(xué)遼寧省

【文章頁(yè)數(shù)】：71 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
ABSTRACT
摘要
Abbreviations list
1. INTRODUCTION
    1.1 Study hypothesis and objectives
    1.2 Biomarkers
2. MATERIALS AND METHODS
    2.1 Materials
    2.2 METHODS
        2.2.1 Cell culture and reagents
        2.2.2 MTT cells toxicity
        2.2.3 Cells Morphology Assay
        2.2.4 Calcien AM Cells Proliferation Assay
        2.2.5 Wound Healing Assay
        2.2.6 Western Blotting Analysis
        2.2.7 Animal model
        2.2.8A nimal Experimental Design and Follow up
3. Statistical Analysis
4. RESULTS
    4.1 The Effect of PCr on Cells Morphology and Structure
    4.2 PCr enhances cells proliferation and decrease cells apoptosis
    4.3 PCr enhances wound healing process
    4.4 PCrameliorates Hepatic Endoplasmic Reticulum Stress markers
    4.5 Effect of PCr on blood glucose level and body mass
    4.6 Effect of PCr on liver biochemical biomarkers
    4.7 Effect of PCr on liver tissues histology
5. DISCUSSION
CONCLUSIONS
Acknowledgements and Research fund
REFERENCES
Literature Review Diabetes mellitus (DM) and Liver Injury
    1.Background
    2.ETYMOLOGY OF DIABETES MELLITUS
    3.Epidemiology
    4.Pathophysiology of type 1 Diabetes Mellitus
        4.1 Role of Insulin in Diabetes Mellitus
        4.2 Factors Causing Diabetes
    5.Complications associated with DM
        5.1 Diabetic retinopathy
        5.2 Glaucoma
        5.3 Cardiovascular diseases associated with diabetes
        5.4 Diabetic nephropathy
        5.5 Diabetic neuropathy
        5.6 Liver injury associated with DM
    6.Biomarkers
    Conclusions and Perspectives
    REFERENCES
APPENDIX-1 Blood glucose and body weight data
PUBLISHED RESEARCH
ACKNOWLEDGEMENT



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