本文選題：種植體周圍炎 + 成骨細(xì)胞��； 參考：《華西口腔醫(yī)學(xué)雜志》2014年02期

【摘要】：目的研究種植體周圍炎條件下炎性微環(huán)境對(duì)頜骨成骨細(xì)胞生物學(xué)功能的影響。方法原代分離培養(yǎng)和純化種植體周圍炎來(lái)源和正常組織來(lái)源的人頜骨成骨細(xì)胞,取第3代細(xì)胞進(jìn)行鑒定和檢測(cè),MTT法檢測(cè)成骨細(xì)胞的增殖能力,實(shí)時(shí)定量聚合酶鏈反應(yīng)法檢測(cè)成骨細(xì)胞相關(guān)基因骨鈣素(OCN)、Runx2、Ⅰ型膠原(ColⅠ)的表達(dá),Western blot法檢測(cè)成骨細(xì)胞相關(guān)蛋白OCN的表達(dá)。結(jié)果從培養(yǎng)第4天起,種植體周圍炎來(lái)源的成骨細(xì)胞的增殖活力明顯低于正常組織來(lái)源的成骨細(xì)胞(P0.05)。培養(yǎng)7 d時(shí),與正常組織來(lái)源的成骨細(xì)胞相比,種植體周圍炎來(lái)源的成骨細(xì)胞相關(guān)基因OCN、Runx2、ColⅠ的表達(dá)均顯著降低(P0.05),相關(guān)蛋白OCN的表達(dá)也顯著降低(P0.05)。結(jié)論種植體周圍炎的炎性微環(huán)境降低了頜骨成骨細(xì)胞的增殖及分化能力。
[Abstract]:Objective to study the effect of inflammatory microenvironment on the biological function of osteoblasts in the condition of periimplant inflammation. Methods Human mandibular osteoblasts derived from periimplant inflammation and normal tissue were isolated and purified. The third generation of osteoblasts were identified and the proliferation of osteoblasts was detected by MTT assay. The expression of osteocalcin (OCN) -associated gene (OCN) Runx2 and collagen 鈪，

本文編號(hào)：2055449