本文選題：建(?) + 髁突　； 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]建立不同周齡SD大鼠動(dòng)物模型,觀察大鼠建(?)前后顳下頜關(guān)節(jié)髁狀突的發(fā)育改建情況以及髁突軟骨中DMP1的表達(dá)規(guī)律,探討咀嚼力變化對(duì)髁突的影響。[方法]實(shí)驗(yàn)一選取新生(0)、3、5、6、8周齡雄性SD大鼠各6只,取右側(cè)髁狀突組織,HE染色觀察大鼠髁突發(fā)育過程中的軟骨厚度、軟骨下骨BV/TV、Tb.Th、Tb.N以及Tb.Sp的變化特征,免疫組化染色檢測(cè)髁突軟骨中DMP1的表達(dá)情況。實(shí)驗(yàn)二選取5周齡雄性SD大鼠共36只,隨機(jī)分配至軟食組以及正常組各18只,適應(yīng)性喂養(yǎng)一周后開始建模,予軟食組大鼠喂粉末狀飼料,正常組大鼠喂普通棒狀飼料,其他飼養(yǎng)條件不變。分別于建模后2、4、6周處死大鼠,HE染色觀察大鼠髁突軟骨厚度、軟骨下骨BV/TV、Tb.Th、Tb.N以及Tb.Sp的變化,免疫組化染色檢測(cè)各組大鼠髁突軟骨中DMP1的表達(dá)情況。采用Image Pro Plus 6.0圖像分析系統(tǒng)進(jìn)行組織學(xué)測(cè)量分析,結(jié)果應(yīng)用SPSS 23.0統(tǒng)計(jì)分析軟件進(jìn)行統(tǒng)計(jì)分析。[結(jié)果]1.實(shí)驗(yàn)一:大鼠髁突軟骨組織學(xué)厚度在0-8周齡期間降低,其中3-8周齡軟骨厚度顯著降低(P0.05),以增殖層逐漸增厚(P0.05),成軟骨細(xì)胞層逐漸變薄為特征(P0.05);軟骨下骨:隨大鼠周齡的增長(zhǎng),BV/TV、Tb.Th均明顯增加(P0.05),而Tb.N呈下降趨勢(shì)(P0.05),Tb.Sp:8w組較3w組雖稍有增加,但差異無統(tǒng)計(jì)學(xué)意義(P0.05);DMP1:主要表達(dá)于髁突軟骨成軟骨細(xì)胞層和肥大層的細(xì)胞內(nèi);大鼠0-8周齡期間,成軟骨細(xì)胞層中DMP1的表達(dá)量呈逐漸降低的趨勢(shì)(P0.05);而肥大層中其表達(dá)量卻逐漸升高(P0.05)。2.實(shí)驗(yàn)二:各個(gè)時(shí)間點(diǎn),軟食組分別與正常組比較,軟食組髁突軟骨厚度明顯下降(P.0.05);軟食組軟骨下骨BV/TV和Tb.Th呈現(xiàn)顯著性下降的態(tài)勢(shì)(P0.05),Tb.N卻呈上升態(tài)勢(shì)(P0.05);Tb.Sp各組間比較,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。DMP1:在8w、10w時(shí),軟食組DMP1表達(dá)量均顯著低于正常組(P0.05);12w時(shí),軟食組DMP1表達(dá)量雖稍低于正常組,但差異無統(tǒng)計(jì)學(xué)意義(P0.05)。[結(jié)論]1.髁突的發(fā)育呈周期性變化。3周齡大鼠(離乳后)髁突軟骨的生長(zhǎng)發(fā)育加速,以軟骨厚度降低、軟骨增殖層厚度增加為特征。6周齡大鼠(建(?)后)軟骨下骨骨化速度加快,以BV/TV、Tb.Th大幅度增加以及Th.N顯著性降低為特征。證明咀嚼應(yīng)力的刺激對(duì)髁突的生長(zhǎng)發(fā)育很有必要。2.軟食喂養(yǎng)沒有導(dǎo)致大鼠體重降低,但其引起的咀嚼力變化可導(dǎo)致髁突骨組織發(fā)生退行性改建,以髁突軟骨厚度及軟骨下骨BV/TV、Tb.Th均降低為特征。進(jìn)一步證實(shí)正常咀嚼力刺激對(duì)于髁突軟骨及軟骨下骨組織形態(tài)維持至關(guān)重要。3.DMP1主要表達(dá)于髁突軟骨成軟骨細(xì)胞層和肥大層的細(xì)胞內(nèi),揭示其在髁突軟骨生長(zhǎng)發(fā)育中發(fā)揮重要作用。軟食喂養(yǎng)引起的咀嚼力變化,可導(dǎo)致髁突軟骨中DMP1的表達(dá)量較正常組顯著降低,推測(cè)DMP1可能參與髁突軟骨改建過程中的生物力學(xué)信號(hào)傳導(dǎo)通路。
[Abstract]:[objective] to establish SD rat models of different weeks of age and observe the establishment of SD rats. The development and remodeling of the condyle of anterior and posterior temporomandibular joint and the expression of DMP1 in condylar cartilage were studied to investigate the effect of the change of masticatory force on the condyle. [methods] in experiment one, 6 male Sprague-Dawley rats aged 8 weeks (0) were selected. The right condylar tissue was stained with HE to observe the thickness of cartilage, the changes of TVB / Tb. Thb.N and Tb.Sp in the subchondral bone of rats during the development of condyle. The expression of DMP1 in condylar cartilage was detected by immunohistochemical staining. In experiment 2, 36 male SD rats aged 5 weeks were randomly assigned to the soft food group and the normal group, 18 rats in each group. After one week of adaptive feeding, the rats in the soft food group were fed with powder feed and the normal group rats were fed with ordinary rod feed. Other feeding conditions remain unchanged. The thickness of condylar cartilage, the changes of subchondral BV / TVT Tb. Tb.N and Tb.Sp were observed by HE staining. The expression of DMP1 in condylar cartilage of rats in each group was detected by immunohistochemical staining. The image Pro Plus 6.0 image analysis system was used for histologic measurement and analysis. The results were analyzed by SPSS 23.0 statistical analysis software. [result] 1. Experiment 1: the histological thickness of condylar cartilage in rats decreased during 0-8 weeks of age. The thickness of cartilage at 3-8 weeks was significantly decreased (P0.05), characterized by the gradual thickening of proliferative layer (P0.05) and the gradual thinning of chondroblast layer (P0.05); the subchondral bone: BV / TVTVTb 路Th significantly increased with the increase of age (P0.05), while Tb.N showed a decreasing trend (P0.05). But the difference was not statistically significant (P0.05): DMP1 was mainly expressed in the chondroblast layer and hypertrophic layer of condylar chondrocytes. The expression of DMP1 in chondroblast layer decreased gradually (P0.05), but increased in hypertrophic layer (P0.05). Experiment 2: at each time point, the thickness of condylar cartilage in soft food group was significantly lower than that in normal group (P.0.05), while BV / TV and Tb 路Th in soft food group were significantly decreased (P0.05), but Tb.N was increased (P0.05). There was no significant difference (P0.05). DMP1 expression in soft food group was significantly lower than that in normal group (P0.05) 12 weeks after 8 weeks (P0.05), but there was no significant difference in DMP1 expression in soft food group (P0.05). [conclusion] 1. The development of condyle showed periodic changes. The growth and development of condylar cartilage was accelerated in 3 weeks old rats (postlactation). The thickness of cartilage decreased and the thickness of proliferative layer of cartilage increased. The ossification of the subchondral bone was accelerated, characterized by the significant increase of Tb.Th and the decrease of Th.N. It is proved that the stimulation of masticatory stress is necessary for the growth and development of condyle. Soft feeding did not result in weight loss, but the change of masticatory power caused by soft feeding could result in degenerative remodeling of condylar bone tissue, which was characterized by the reduction of condylar cartilage thickness and the decrease of BV / TVV Tb.Th in subchondral bone. It was further confirmed that normal masticatory stimulation was essential to the morphological maintenance of condylar cartilage and subchondral bone. 3. DMP1 was mainly expressed in the chondroblast layer and hypertrophic layer of condylar cartilage. It is revealed that it plays an important role in the growth and development of condylar cartilage. The change of masticatory power induced by soft food feeding resulted in a significant decrease in the expression of DMP1 in condylar cartilage, which suggested that DMP1 might be involved in biomechanical signal transduction pathway during condylar cartilage remodeling.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R782.6

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