本文選題：豬瘟 + 豬繁殖與呼吸綜合征�。� 參考：《江西農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：豬瘟(CSF)、豬繁殖與呼吸綜合征(PRRS)和豬偽狂犬病(PR)是嚴(yán)重危害養(yǎng)豬業(yè)的三大重要疾病,給養(yǎng)豬業(yè)造成了巨大的經(jīng)濟(jì)損失。為了解江西部分地區(qū)豬瘟(CSF)、豬繁殖與呼吸綜合征(PRRS)、豬偽狂犬病(PR)的發(fā)病及疫苗免疫情況,本研究對(duì)上述三種病進(jìn)行了病原學(xué)調(diào)查和血清學(xué)調(diào)查,從而為江西地區(qū)豬瘟、豬繁殖與呼吸綜合征和豬偽狂犬病的防制提供參考。主要內(nèi)容如下:1江西部分地區(qū)CSF、PRRS和PR的病原學(xué)調(diào)查應(yīng)用PCR和RT-PCR方法,對(duì)2014-2016年江西各地區(qū)豬場(chǎng)送檢的病料,分別檢測(cè)CSFV、PRRSV/HP-PRRSV和PRV,并結(jié)合2012-2016年三種病的檢測(cè)結(jié)果進(jìn)行分析。結(jié)果顯示:CSFV陽(yáng)性率為54.9%,各年份陽(yáng)性率從32.2%-73.1%不等,CSFV感染季節(jié)性不明顯,四季陽(yáng)性率從46.7%-63.4%不等;PRRSV陽(yáng)性率為52.7%,各年份陽(yáng)性率分布在36.1%-70.2%之間,PRRSV在秋季感染率較低;HP-PRRSV陽(yáng)性率為33.2%,2013-2016年陽(yáng)性率逐年上升,季節(jié)性不明顯,各季節(jié)陽(yáng)性率分布在26.9%-37.6%之間,秋季感染率最低為26.9%,其他季節(jié)感染率相近;PRV陽(yáng)性率為32.5%,各年份陽(yáng)性率差異不明顯,從31%-37.6%不等,PRV感染季節(jié)性明顯,春季與其他季節(jié)感染率差異明顯。三種病均以混合感染為主,雙重感染率和三重感染率分別為38%、8%,雙重感染主要為“CSF+PRRS”。2江西部分地區(qū)豬群CSF、PRRS及PR的血清學(xué)調(diào)查應(yīng)用酶聯(lián)免疫吸附試驗(yàn)(ELISA)方法,對(duì)江西部分地區(qū)送檢的血清,分別進(jìn)行CSF、PRRS和PRV-gB/gE抗體的檢測(cè)。2014年1月-2016年12月間,共檢測(cè)CSF抗體8826份,PRRS抗體8138份,PRV-gB抗體5819份,PRV-gE抗體6786份。結(jié)果顯示:CSF、PRRS、PRV-gB和PRV-gE抗體的群體陽(yáng)性率分別為72.6%、81.4%、91.7%和31.9%。CSF和PRV-gB抗體陽(yáng)性率逐年上升,PRRS和PRV-gE抗體陽(yáng)性率逐年下降。豬群CSF、PRV-gB/gE抗體陽(yáng)性率冬季最低,PRRS抗體陽(yáng)性率秋季最低。CSF抗體各階段豬群陽(yáng)性率分布在47.6%-85.1%,陽(yáng)性率由高到低依次為:公豬85.1%、母豬84.3%、哺乳仔豬66.6%、育肥豬55.1%、保育豬最低為47.6%。PRRS抗體各階段豬群陽(yáng)性率范圍為72.5%-88.8%,陽(yáng)性率從高到低依次為:育肥豬88.8%、母豬84%、公豬81.1%、哺乳仔豬73.6%、保育豬72.5%。PRV-gB抗體各階段陽(yáng)性率從69.1%-98.5%不等,哺乳仔豬陽(yáng)性率最高為98.5%,其次分別為:母豬97.8%、公豬94.6%、保育豬89.5%和育肥豬69.1%。PRV-gE抗體各階段豬群陽(yáng)性率范圍為24.5%-40.3%,由高到低依次為:哺乳仔豬40.3%、保育豬34.7%、育肥豬31.1%、母豬29.9%、公豬25.5%。大規(guī)�；i場(chǎng)豬群CSF、PRRS、PRV-gB抗體陽(yáng)性率較中小規(guī)�；i場(chǎng)高,PRV-gE抗體陽(yáng)性率較低。
[Abstract]:CSF, PRRSs and PRPRs are the three major diseases that seriously harm the pig industry and cause huge economic losses to the pig industry. In order to understand the incidence and vaccine immunity of CSFN, PRRSs, PRPRs in some areas of Jiangxi Province, the etiology and serological investigation of the above three diseases were carried out in this study, so as to investigate the swine fever in Jiangxi. Prevention and control of porcine reproductive and respiratory syndrome and porcine pseudorabies are provided. The main contents are as follows: 1 PCR and RT-PCR methods were used to investigate the etiology of CSFV PRRS and PR in some parts of Jiangxi Province. The samples from pig farms in Jiangxi from 2014 to 2016 were tested for CSFVV / PRRSVR / HP-PRRSV and PRV respectively, and the results of the three diseases from 2012-2016 were analyzed. The results showed that the positive rate of 1% CSFV was 54.9%, and the positive rate of CSFV varied from 32.2-1% to 73.1% in different years. In the four seasons, the positive rate of PRRSV ranged from 46.7% to 63.4%. The positive rate of PRRSV was between 36.1% and 70.2% in each year. The positive rate of HP-PRRSV in autumn was 33.2%. The positive rate of HP-PRRSV increased year by year, and the seasonal rate was not obvious. The positive rate of PRRSV in each season was between 26.9- 37.6%. The lowest infection rate was 26.9m in autumn, and the positive rate of PRV in other seasons was 32.5. There was no significant difference in the positive rate of PRV from 31% to 37.6% in different years. The infection rate in spring was obviously different from that in other seasons. The double infection rate and triple infection rate were 38% and 8% respectively. The double infection rate was mainly "CSF PRRS" .2 the serological investigation of CSFU PRRS and PR in some parts of Jiangxi Province was carried out by Elisa method. From January 2014 to December 2016, 8826 samples of CSF antibodies were detected, 8138 samples of CSF antibodies were detected, 5819 samples of PRV-gB antibodies and 6786 samples of PRV-gE antibodies were detected. The results showed that the positive rates of PRV-gB and PRV-gE antibodies were 72.6% and 91.7%, respectively, and the positive rates of 31.9%.CSF and PRV-gB antibodies increased year by year. The positive rates of PRRS and PRV-gE antibodies decreased year by year. The positive rate of PRV-gB- GE antibody was the lowest in winter and the lowest in autumn. The positive rate of PRRS antibody in all stages was 47.6-85.1. The positive rate was 85.1% in male pig, 84.3% in sows, 66.6% in lactation piglets, 55.1% in fattening pigs, and the lowest in 47.6%.PRRS in conservation pigs. The order of positive rate was as follows: 85.1% in boar, 84.3% in sows, 66.6% in lactating piglets, 55.1% in fattening pigs, and the lowest in preserved pigs. The positive rate of antibody in different stages was 72.5-88.88.The positive rate ranged from high to low as follows: 88. 8 of fattening pigs, 84 of sows, 81.1 of boars, 73.6 of suckling piglets, and 69.1-98.5% of 72.5%.PRV-gB antibodies in all stages of care. The positive rate of breast-feeding piglets was 98.5am, followed by 97.8g sows, 94.6g boar pigs, 89.5% of preserved pigs and 24.5-40.3of fattening pigs in each stage of 69.1%.PRV-gE antibody. The order from high to low was as follows: nursing piglets 40.33,34.7care pigs, fattening pigs 31.1m, sows 29.995, boar 25.5. The positive rate of PRV-gB antibody in large scale pig farm was lower than that in medium and small scale pig farm.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.28

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