【摘要】：目的:通過(guò)對(duì)大鼠進(jìn)行長(zhǎng)期大強(qiáng)度運(yùn)動(dòng)和鼠李糖乳桿菌的干預(yù),觀察小腸部分益生菌的定植情況,以及反映消化、吸收、屏障等功能的部分指標(biāo),發(fā)現(xiàn)運(yùn)動(dòng)應(yīng)激與小腸之間的聯(lián)系,并且探討益生菌的補(bǔ)充在運(yùn)動(dòng)領(lǐng)域中應(yīng)用的問(wèn)題。方法:SD大鼠40只隨機(jī)分為4組。安靜營(yíng)養(yǎng)組和運(yùn)動(dòng)營(yíng)養(yǎng)組每日灌胃鼠李糖乳桿菌溶液2m L。運(yùn)動(dòng)對(duì)照組和運(yùn)動(dòng)營(yíng)養(yǎng)組在跑臺(tái)上進(jìn)行9周的大強(qiáng)度訓(xùn)練。訓(xùn)練最后一周采集大鼠糞便及尿液,測(cè)定糞便細(xì)菌含量、尿液D-木糖濃度。最后一次訓(xùn)練結(jié)束后24小時(shí)麻醉處死,測(cè)定血清、小腸組織中指標(biāo)變化。統(tǒng)計(jì)學(xué)分析采用雙因素方差分析。結(jié)果:運(yùn)動(dòng)對(duì)照組血清ACTH水平高于安靜對(duì)照組(P0.01),安靜營(yíng)養(yǎng)組低于運(yùn)動(dòng)營(yíng)養(yǎng)組(P0.05);運(yùn)動(dòng)對(duì)照組、安靜營(yíng)養(yǎng)組雙歧桿菌低于安靜對(duì)照組(P0.01);運(yùn)動(dòng)對(duì)照組小腸組織5-HT水平高于安靜對(duì)照組(P0.05),血清5-HIAA安靜對(duì)照組均低于安靜營(yíng)養(yǎng)組和運(yùn)動(dòng)對(duì)照組(P0.01);安靜營(yíng)養(yǎng)組SERT蛋白表達(dá)水平低于安靜對(duì)照組(P0.05)和運(yùn)動(dòng)營(yíng)養(yǎng)組(P0.05);小腸s Ig A方面,運(yùn)動(dòng)營(yíng)養(yǎng)組和運(yùn)動(dòng)對(duì)照組均高于安靜對(duì)照組(P0.01);小腸IL-8方面,運(yùn)動(dòng)對(duì)照組高于安靜對(duì)照組(P0.05);血清LPS安靜營(yíng)養(yǎng)組高于安靜對(duì)照組(P0.05)和運(yùn)動(dòng)對(duì)照組(P0.01),運(yùn)動(dòng)對(duì)照組低于運(yùn)動(dòng)營(yíng)養(yǎng)組(P0.05);與運(yùn)動(dòng)對(duì)照組相比,運(yùn)動(dòng)營(yíng)養(yǎng)組MUC2表達(dá)增加(P0.05);安靜對(duì)照組小腸固有層厚度均小于安靜營(yíng)養(yǎng)組和運(yùn)動(dòng)對(duì)照組(P0.01);運(yùn)動(dòng)對(duì)照組小腸絨毛高度小于安靜對(duì)照組(P0.01)、運(yùn)動(dòng)營(yíng)養(yǎng)組(P0.05),安靜營(yíng)養(yǎng)組的絨毛寬度高于安靜對(duì)照組(P0.05)。結(jié)論:1.本研究采用的9周大強(qiáng)度運(yùn)動(dòng)對(duì)大鼠造成了一定程度的腸道功能紊亂,體現(xiàn)為定植的雙歧桿菌減少,小腸5-HT代謝異常,預(yù)示腸道消化功能存在潛在風(fēng)險(xiǎn)。2.大鼠小腸組織的糖轉(zhuǎn)運(yùn)機(jī)制和吸收效率未受到本研究中運(yùn)動(dòng)應(yīng)激的影響,粘膜屏障無(wú)明顯破壞,未出現(xiàn)病理性變化,但仍存在一定炎癥風(fēng)險(xiǎn)。3.研究中所采用的鼠李糖乳桿菌并不能顯著改善所出現(xiàn)的功能紊亂,也未影響糖吸收效率和轉(zhuǎn)運(yùn)機(jī)制,并且與運(yùn)動(dòng)應(yīng)激、SERT蛋白表達(dá)之間存在相互影響的復(fù)雜關(guān)系。長(zhǎng)期補(bǔ)充會(huì)影響小腸粘膜,細(xì)菌移位增加,需辯證考慮益生菌的使用。
[Abstract]:Objective: to observe the colonization of some probiotics in the small intestine and some indexes reflecting the function of digestion, absorption and barrier through the long-term intensive exercise and the intervention of Lactobacillus rhamnoides in rats. To explore the relationship between exercise stress and small intestine and to explore the application of probiotic supplement in sports field. Methods 40 Sprague-Dawley rats were randomly divided into 4 groups. Lactobacillus rhamnoides solution was administered daily to rest nutrition group and exercise nutrition group (2m L). The exercise control group and the exercise nutrition group were trained for 9 weeks on the platform. The feces and urine of rats were collected at the end of the training week, and the fecal bacterial content and urine D-xylose concentration were determined. The indexes in serum and small intestine were measured 24 hours after the last training. Two-factor ANOVA was used for statistical analysis. Results: the level of serum ACTH in exercise control group was higher than that in quiet control group (P0.01), and that in quiet nutrition group was lower than that in exercise nutrition group (P0.05). The levels of 5-HT in small intestine in the exercise control group were higher than those in the quiet control group (P0.05), and the levels of serum 5-HIAA in the quiet control group were lower than those in the quiet nutrition group and the exercise control group (P0.01); the expression of SERT protein in the quiet nutrition group was higher than that in the rest nutrition group (P0.01). The level of small intestine s Ig A was lower than that of quiet control group (P0.05) and exercise nutrition group (P0.05). Exercise nutrition group and exercise control group were higher than quiet control group (P0.01), small intestine IL-8, Exercise control group was higher than quiet control group (P0.05), serum LPS quiet nutrition group was higher than quiet control group (P0.05) and exercise control group (P0.01), exercise control group was lower than exercise nutrition group (P0.05); The expression of MUC2 was increased in exercise nutrition group (P0.05), the thickness of lamina propria of small intestine in quiet control group was lower than that in quiet nutrition group and exercise control group (P0.01), the villi height of small intestine in exercise control group was lower than that in quiet control group (P0.01), and that in exercise nutrition group (P0.05). The hair width was higher than that in the quiet control group (P0.05). Conclusion 1. In this study, 9 weeks of intensive exercise caused a certain degree of intestinal dysfunction in rats, which was reflected in the reduction of bifidobacterium colonization and the abnormal metabolism of 5-HT in the small intestine, which indicated that there was a potential risk of intestinal digestive function. The mechanism of glucose transport and absorption efficiency of small intestine in rats were not affected by the exercise stress in this study, the mucosal barrier was not obviously damaged, and no pathological changes occurred, but there was still a certain inflammatory risk. 3. Lactobacillus rhamnoides used in the study did not significantly improve the functional disorder, nor did it affect the glucose absorption efficiency and transport mechanism, and there was a complex relationship between the effects of rhamnobacterium rhamnoides and the expression of SERT protein under exercise stress. Long-term supplementation will affect intestinal mucosa and increase bacterial translocation. The use of probiotics should be considered dialectically.
【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：G804.2

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