發(fā)布時間：2019-05-21 21:54

【摘要】：(1)均苯四甲酸是芳香族羧酸化合物中應用最為廣泛的化合物之一,在化工合成及高分子行業(yè)中具有重要的作用。高濃度的均苯四甲酸廢水因其可生化性差、COD值高、酸性強等特點,會給后續(xù)的污水處理帶來很大困難,給自然環(huán)境造成很大危害。本研究從長青農(nóng)藥廠污水處理站的活性污泥中分離到了 一株能夠高效降解均苯四甲酸的菌株將其命名為PB-3,該菌株能以均苯四甲酸為唯一碳源和能源進行生長。根據(jù)16S rRNA序列同源性分析,并結(jié)合菌株的表型特征和生理生化特性,將菌株PB-3初步鑒定為 Arthrobacter phenanthrenivorans。(2)菌株PB-3在LB培養(yǎng)基中生長良好。0～4h為菌株PB-3的延滯期,4～20h為菌株的對數(shù)期,菌株開始迅速生長,20 h以后開始進入穩(wěn)定期。LB培養(yǎng)基中菌株的最適溫度為30℃,最佳pH值為7.0。菌株為好氧菌,其生長量與裝液量呈負相關。PB-3的最適鹽濃度為10 g/L。在碳源利用試驗中,菌株PB-3對葡萄糖和蔗糖的利用率較高,其次為果糖、甘露醇、木糖,幾乎不能利用乳糖來進行生長;在氮源利用試驗中,蛋白胨為氮源時,菌株PB-3長勢最優(yōu),其次分別是氯化銨、硝酸鉀、硫酸銨和尿素。在抗生素的耐受性試驗中,菌株只對諾氟沙星具有抗性,對四環(huán)素、慶大霉素、卡那霉素、氯霉素、頭孢曲松、青霉素、氧氟沙星、羧芐西林、氨芐西林、壯觀霉素、鏈霉素均較為敏感。(3)菌株PB-3在無機鹽培養(yǎng)基中的生長降解曲線表明,在64 h后PB-3能將400 mg/L的均苯四甲酸完全降解,并隨著底物的降解,菌株的生長量逐步增加。在單因素試驗中,PB-3降解均苯四甲酸的最適溫度為30℃,最適pH為7.0。當均苯四甲酸的初始濃度從200 mg/L增加到1000 mg/L時,對菌株的降解能力沒有明顯的抑制作用�？梢赃m當通過提高菌株PB-3的接種量來加快降解均苯四甲酸的速度。隨著培養(yǎng)基中NaCl的濃度逐漸提高,對菌株降解均苯四甲酸能力的抑制越為強烈。以添加葡萄糖為外加碳源時,會延緩菌株對均苯四甲酸的降解。PB-3能夠以1,2,4-苯三甲酸、鄰苯二甲酸為唯一碳源并能夠?qū)⑵淇焖俳到?但對間苯二甲酸的降解速度較為緩慢,且不能利用均苯三甲酸為碳源進行生長。PB-3對均苯四甲酸的降解符合Monod方程,其降解動力學方程為V=47.6S/(181 +S)。(4)通過LC-MS對菌株代謝均苯四甲酸的代謝中間體進行了分析,檢測到了三種主要的代謝產(chǎn)物,分別為1,2,4-苯三甲酸、對苯二甲酸和2,5-二羥基對苯二甲酸。并對其代謝途徑進行了初步推斷。
[Abstract]:The main results are as follows: (1) phenyltetracarboxylic acid is one of the most widely used aromatic carboxylic acid compounds, which plays an important role in chemical synthesis and polymer industry. Because of its poor biodegradability, high COD value and strong acidity, high concentration of phenylenetetraformic acid wastewater will bring great difficulties to the subsequent sewage treatment and cause great harm to the natural environment. In this study, a strain named PB-3, was isolated from the activated sludge of sewage treatment station of Changqing Pesticide Plant, which can grow with phenyltetracarboxylic acid as the sole carbon source and energy source. According to the homology analysis of 16s rRNA sequence, combined with the phenotypic and physiological and biochemical characteristics of the strain, strain PB-3 was identified as Arthrobacter phenanthrenivorans. (2) strain PB-3 grew well in LB medium. 0 ~ 4 h was the lag period of strain PB-3. 4 鈮，

本文編號：2482432