【摘要】：建立同時測定蒙藥復(fù)方述達格-4中山奈酚、高良姜素、山奈素、高良姜素-3-甲醚含量的方法.采用HPLC法,色譜柱為AgilentC18色譜柱(4.6mm×250mm,5μm),流動相乙腈-0.2%乙酸水溶液(梯度洗脫),流速0.6mL·min-1,檢測波長254nm,柱溫30℃,進樣量為10μL.得出山奈酚、高良姜素、山奈素和高良姜素-3-甲醚線性范圍分別為0.00067-0.00670μg(r=0.9999)、0.5240-5.2400μg(r=0.9999)、0.1484-1.4840μg(r=0.9999)、0.0996-0.9960μg(r=0.9999);精密度、穩(wěn)定性、重復(fù)性試驗的RSD2%;平均加樣回收率分別為96.79%(RSD=1.70%,n=6)、97.38%(RSD=1.59%,n=6)、100.96%(RSD=1.49%,n=6)、97.01%(RSD=1.50%,n=6).結(jié)果表明該方法操作簡便、穩(wěn)定、重復(fù)性好,可用于蒙藥復(fù)方述達格-4中4個黃酮成分含量的測定.本研究可為蒙藥復(fù)方述達格-4資源開發(fā)利用提供理論依據(jù).
[Abstract]:A method was developed for the simultaneous determination of Zhongshan phenol, kaempferol, kaempferol and galangin-3-methyl ether in Mongolian medicine compound Zudag-4. A HPLC method was used with a AgilentC18 column (4.6mm 脳 250 mm, 5 渭 m), a mobile phase acetonitrile-0.2% acetic acid solution (gradient elution), a flow rate of 0.6mL 路min-1, a detection wavelength of 254 nm, a column temperature of 30 鈩，

本文編號：2467017