本文選題：原發(fā)性痛經(jīng) + 寒濕凝滯型　； 參考：《河北醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:觀察不同時(shí)機(jī)艾灸干預(yù)對(duì)寒濕凝滯型原發(fā)性痛經(jīng)(Primary Dysmenorrhea,PD)大鼠行為學(xué)、子宮組織微血管密度(MVD)、子宮組織前列腺素F2α(PGF2α)、前列腺素E2(PGE2)、加壓素(AVP)、一氧化氮(NO)、內(nèi)皮素(ET-1)含量以及血漿血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)含量的影響,以探討其對(duì)寒濕凝滯型原發(fā)性痛經(jīng)模型大鼠止痛效應(yīng)影響的差異性及其可能的作用機(jī)制。以期為臨床選擇最佳時(shí)機(jī)的艾灸介入治療寒濕凝滯型原發(fā)性痛經(jīng)提供可靠的實(shí)驗(yàn)依據(jù)。方法:將60只Wistar雌性大鼠適應(yīng)性喂養(yǎng)7 d后,采用陰道涂片法,篩選出處于動(dòng)情間期大鼠45只,并將其隨機(jī)分為對(duì)照組(Control)、模型組(Model)、預(yù)先灸組(Pre-Mox)、即刻灸組(Im-Mox)和預(yù)先即刻灸組(PI-Mox)(每組9只)。對(duì)照組于大鼠股部給予皮下注射生理鹽水,1次/d,連續(xù)10 d(第1 d、10 d注射0.5 m L/只,第2~9 d 0.2 m L/只)。其余4組大鼠均采用(0±1)℃冰水浴結(jié)合苯甲酸雌二醇注射法制備寒濕凝滯型痛經(jīng)模型,將大鼠后肢及下腹部浸入(0±1)℃冰水混合物中(室溫23±2℃)進(jìn)行寒冷刺激,1次/d,20 min/次,同時(shí)給予大鼠股部皮下注射苯甲酸雌二醇,1次/d,連續(xù)10 d(第1 d、10 d注射0.5 mg/只,第2~9 d 0.2 mg/只)。預(yù)先灸組于造模期間第8 d開始治療,第10 d結(jié)束治療,共3 d;即刻灸組于造模第11 d注射完縮宮素后(即痛經(jīng)模型復(fù)制成功后)開始治療,共1 d;預(yù)先即刻灸組于造模期間第8 d開始治療,第11 d結(jié)束治療,共4 d。3個(gè)艾灸組均采用直徑為7 mm粗細(xì)的艾條,于神闕、關(guān)元穴處同時(shí)施溫和灸,10 min/穴,1次/d。對(duì)照組、模型組均采用與艾灸組相同的體位固定,10 min/次,1次/d。除對(duì)照組外,其余4組寒濕凝滯型PD模型大鼠模型均注射縮宮素后觀察其行為學(xué)反應(yīng),待行為學(xué)檢測(cè)結(jié)束后,將大鼠斷頭取血后,于冰盤上迅速分離出“Y”字形子宮左側(cè)子宮組織,以冷藏的生理鹽水洗凈拭干稱重,加入生理鹽水碾磨,制成30%的子宮組織勻漿,于低溫4℃時(shí)3000 rpm離心15 min,取上清液,用放射免疫分析法檢測(cè)大鼠子宮組織PGE2、AVP、ET-1含量,酶聯(lián)免疫法檢測(cè)大鼠子宮組織PGF2α含量,生物化學(xué)技術(shù)檢測(cè)大鼠子宮組織NO含量。右側(cè)子宮組織用濃度為4%的多聚甲醛溶液進(jìn)行固定,石蠟包埋、切片,一部分采用免疫組化法檢測(cè)子宮組織MVD,剩余部分用于HE染色,于光鏡下觀察各組大鼠子宮組織MVD和子宮組織細(xì)胞的形態(tài)學(xué)改變。結(jié)果:1不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠行為學(xué)的影響扭體行為學(xué)觀察結(jié)果顯示:艾灸干預(yù)后,預(yù)先灸組、即刻灸組、預(yù)先即刻灸組痛經(jīng)大鼠的潛伏期均明顯延長(zhǎng)、扭體次數(shù)減少、扭體總分降低,與模型組比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.01);3個(gè)艾灸組相比,即刻灸組、預(yù)先即刻灸組較預(yù)先灸組痛經(jīng)大鼠的扭體次數(shù)及扭體總分均明顯減少和降低(P0.01),預(yù)先即刻灸組較預(yù)先灸組痛經(jīng)大鼠的潛伏期明顯延長(zhǎng)(P0.01);預(yù)先即刻灸組與即刻灸組比較,預(yù)先即刻灸組較即刻灸組痛經(jīng)大鼠的潛伏期明顯延長(zhǎng)、扭體次數(shù)及扭體總分均明顯減少和降低(P0.05或P0.01)。2不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠子宮組織細(xì)胞病理形態(tài)學(xué)的影響子宮組織HE染色后,置于光鏡下,一般形態(tài)學(xué)觀察顯示:對(duì)照組大鼠子宮組織形態(tài)規(guī)則,無水腫,大量中性粒細(xì)胞;模型組大鼠子宮組織形態(tài)較規(guī)則,組織嚴(yán)重水腫,充血,大量中性粒細(xì)胞和淋巴細(xì)胞;預(yù)先灸組、即刻灸組、預(yù)先即刻灸組大鼠子宮組織均形態(tài)規(guī)則,組織輕度水腫,無充血,大量中性粒細(xì)胞,少量淋巴細(xì)胞。3不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠子宮組織MVD值的影響模型組大鼠子宮組織MVD明顯減少,與對(duì)照組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05);預(yù)先灸組、即刻灸組、預(yù)先即刻灸組與模型組比較,3個(gè)艾灸組痛經(jīng)大鼠子宮組織MVD均明顯增多,差異有統(tǒng)計(jì)學(xué)意義(P0.01);預(yù)先即刻灸組與預(yù)先灸組比較,預(yù)先即刻灸組較預(yù)先灸組MVD明顯增多(P0.05);即刻灸組與預(yù)先灸組比較,即刻灸組較預(yù)先灸組MVD雖有數(shù)值差異,但無統(tǒng)計(jì)學(xué)差異(P0.05)。預(yù)先即刻灸組與即刻灸組比較,預(yù)先即刻灸組較即刻灸組雖有數(shù)值差異,但無統(tǒng)計(jì)學(xué)差異(P0.05)。4不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠血漿TXB2和6-keto-PGF1α含量的影響模型組痛經(jīng)大鼠血漿TXB2含量及TXB2/6-keto-PGF1α比值均明顯升高、6-keto-PGF1α含量明顯降低,與對(duì)照組相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.01);艾灸干預(yù)后,預(yù)先灸組、即刻灸組、預(yù)先即刻灸組痛經(jīng)大鼠血漿TXB2含量及TXB2/6-keto-PGF1α比值均明顯降低、6-keto-PGF1α含量明顯升高,與模型組相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.01);預(yù)先即刻灸組與預(yù)先灸組比較,預(yù)先即刻灸組較預(yù)先灸組痛經(jīng)大鼠血漿TXB2含量及TXB2/6-keto-PGF1α比值均明顯降低,6-keto-PGF1α含量明顯升高(P0.01或P0.05);即刻灸組與預(yù)先灸組比較,即刻灸組較預(yù)先灸組血漿TXB2、6-keto-PGF1α含量及TXB2/6-keto-PGF1α比值雖有數(shù)值差異,但無統(tǒng)計(jì)學(xué)意義(P0.05);預(yù)先即刻灸組與即刻灸組比較,預(yù)先即刻灸組較即刻灸組痛經(jīng)大鼠血漿TXB2含量及TXB2/6-keto-PGF1α比值均明顯降低、6-keto-PGF1α含量明顯升高(P0.01)。5不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠子宮組織PGF2α、PGE2含量及PGF2α/PGE2比值的影響模型組痛經(jīng)大鼠子宮組織PGF2α的含量及PGF2α/PGE2比值均明顯升高,PGE2的含量明顯降低,與對(duì)照組比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。艾灸干預(yù)后,預(yù)先灸組、即刻灸組、預(yù)先即刻灸組痛經(jīng)大鼠子宮組織PGF2α的含量及PGF2α/PGE2比值均明顯降低、PGE2含量明顯升高,與模型組相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.01或P0.05)。預(yù)先即刻灸組與預(yù)先灸組比較,預(yù)先即刻灸組較預(yù)先灸組子宮組織PGF2α的含量及PGF2α/PGE2比值均明顯降低(P0.01),PGE2的含量明顯升高(P0.01)。即刻灸組與預(yù)先灸組比較,即刻灸組較預(yù)先灸組PGF2α的含量及PGF2α/PGE2比值均明顯降低(P0.05),PGE2含量明顯升高(P0.01)。預(yù)先即刻灸組與即刻灸組比較,預(yù)先即刻灸組較刻灸組痛經(jīng)大鼠子宮組織PGF2α的含量及PGF2α/PGE2比值均明顯降低(P0.01),PGE2的含量明顯升高(P0.01)。6不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠子宮組織AVP含量的影響模型組痛經(jīng)大鼠子宮組織AVP的含量明顯升高,與對(duì)照組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。艾灸干預(yù)后,預(yù)先灸組、即刻灸組、預(yù)先即刻灸組3個(gè)艾灸組痛經(jīng)大鼠子宮組織AVP含量均明顯降低,與模型組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01)。預(yù)先即刻灸組、即刻灸組、預(yù)先灸組3組兩兩比較,3組子宮組織AVP含量雖各有數(shù)值差異,但無統(tǒng)計(jì)學(xué)意義(P0.05)。7不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD大鼠子宮組織ET-1、NO含量的影響模型組痛經(jīng)大鼠子宮組織ET-1的含量明顯升高,NO的含量明顯降低,與對(duì)照組比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。艾灸干預(yù)后,預(yù)先灸組、即刻灸組、預(yù)先即刻灸組痛經(jīng)大鼠子宮組織ET-1的含量明顯降低、NO含量明顯升高,與模型組相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。預(yù)先即刻灸組與預(yù)先灸組比較,預(yù)先即刻灸組較預(yù)先灸組子宮組織ET-1的含量明顯降低、NO的含量明顯升高(P0.01)。即刻灸組與預(yù)先灸組比較,即刻灸組較預(yù)先灸組ET-1的含量明顯降低、NO含量明顯升高(P0.05)。預(yù)先即刻灸組與即刻灸組比較,預(yù)先即刻灸組較刻灸組痛經(jīng)大鼠子宮組織ET-1的含量明顯降低、NO的含量明顯升高(P0.05)。結(jié)論:1預(yù)先灸、即刻灸及預(yù)先即刻灸的不同時(shí)機(jī)介入均可明顯減輕寒濕凝滯型PD大鼠腹部收縮及扭體反應(yīng),明顯延長(zhǎng)其潛伏期,但其效果尤以預(yù)先即刻灸最為明顯,即刻灸次之。說明不同時(shí)機(jī)艾灸對(duì)寒濕凝滯型PD模型大鼠均有良好的鎮(zhèn)痛效果,但預(yù)先即刻灸的效果更加顯著。2三種不同時(shí)機(jī)艾灸均可改善寒濕凝滯型PD模型大鼠子宮組織充血、水腫狀態(tài)。但預(yù)先灸、即刻灸及預(yù)先即刻灸3組之間對(duì)大鼠子宮組織病理形態(tài)學(xué)改變無明顯差異。3三種不同時(shí)機(jī)艾灸均可增加寒濕凝滯型PD模型大鼠子宮組織MVD,有效改善子宮局部微循環(huán)。但其中尤以預(yù)先即刻灸組效果最佳,明顯優(yōu)于預(yù)先灸和即刻灸。其作用可能是通過艾火的溫通、經(jīng)絡(luò)的傳熱及神經(jīng)的傳遞對(duì)子宮產(chǎn)生的熱效應(yīng),以增加血流量,改變局部血液循環(huán),進(jìn)而達(dá)到緩解疼痛之目的。64三種不同時(shí)機(jī)艾灸均可降低寒濕凝滯型PD模型大鼠血漿TXB2含量及TXB2/6-keto-PGF1α比值;升高6-keto-PGF1α的含量,其中尤以預(yù)先即刻灸最為明顯。說明不同時(shí)機(jī)艾灸對(duì)上述各項(xiàng)指標(biāo)水平均有良好的調(diào)節(jié)作用,但預(yù)先即刻灸的調(diào)節(jié)作用更加顯著。其作用機(jī)制可能是通過調(diào)節(jié)血小板活性和血管張力,改善血管收縮狀態(tài),使子宮局部血流量增加,而達(dá)到止痛之目的。5三種不同時(shí)機(jī)艾灸均可降低寒濕凝滯型PD模型大鼠子宮組織PGF2α含量及PGF2α/PGE2比值;提高PGE2的水平,但其作用尤以預(yù)先即刻灸最為明顯,即刻灸次之。說明不同時(shí)機(jī)艾灸對(duì)PGF2α和PGE2的異常水平均有良好的調(diào)節(jié)作用,且預(yù)先即刻灸的調(diào)節(jié)作用更加顯著,其作用機(jī)制可能是通過恢復(fù)PGF2α和PGE2的平衡,改善機(jī)體內(nèi)分泌環(huán)境,進(jìn)而抑制子宮平滑肌及血管痙攣性收縮,改善局部的缺血缺氧狀態(tài),從而起到鎮(zhèn)痛作用。6三種不同時(shí)機(jī)艾灸均可明顯降低寒濕凝滯型PD模型大鼠子宮組織AVP含量,且三者之間無明顯差異。其作用機(jī)制可能是通過降低AVP含量,進(jìn)一步減少PG合成與釋放,從而有效抑制子宮平滑肌痙攣性收縮,改善局部血液循環(huán),增加血流量,緩解缺血狀態(tài),從而發(fā)揮其鎮(zhèn)痛之效應(yīng)。7三種不同時(shí)機(jī)艾灸均可降低ET-1含量、升高NO含量,其中預(yù)先即刻灸最為明顯,即刻灸次之。說明不同時(shí)機(jī)艾灸均可調(diào)節(jié)子宮組織ET-1和NO的失衡狀態(tài),且預(yù)先即刻灸的調(diào)節(jié)作用更加顯著。其作用機(jī)制可能是通過舒張子宮血管平滑肌,抑制其收縮,改善子宮組織微循環(huán),增加其血流量,以達(dá)到緩解疼痛之目的。
[Abstract]:Objective: To observe the behavior of Primary Dysmenorrhea (PD), uterine tissue microvascular density (MVD), prostaglandin F2 alpha (PGF2 a), prostaglandin E2 (PGE2), vasopressin (AVP), nitrogen monoxide (NO), endothelin (ET-1), plasma thromboxane (thromboxane), and plasma thromboxane (PD). The effect of the content of adenine F1 alpha (6-keto-PGF1 alpha) on the difference and possible mechanism of its effect on the analgesic effect of the cold wet and stagnant primary dysmenorrhea model rats is discussed in order to provide the experimental basis for the best time to choose the best time to treat the cold wet and stagnant primary dysmenorrhea by the moxibustion intervention. Method: 60 females of Wistar are large. After 7 d of adaptive feeding, 45 rats were selected by vaginal smear and divided into control group (Control), model group (Model), pre moxibustion group (Pre-Mox), immediate moxibustion group (Im-Mox) and pre moxibustion group (PI-Mox) (9 in each group). The control group was given subcutaneous injection of saline, 1 times /d, and 10 d in the control group. (first D, 10 d injection 0.5 m L/, 2~9 D 0.2 m L/). The remaining 4 groups of rats were used (0 + 1) centigrade ice water bath combined with estradiol benzoate to prepare the cold wet and stagnant dysmenorrhea model. The rats' hind limbs and lower abdomen were immersed in (0 + 1) C ice water mixture (room temperature 23 + 2 c) for cold stimulation, 1 times /d, 20 min/ times, and rat share at the same time. The subcutaneous injection of estradiol benzoate, 1 /d, continuous 10 d (first D, 10 d injection 0.5 mg/, 2~9 D 0.2 mg/). The pre moxibustion group was treated with eighth d during the mould making, tenth D ended with a total of 3 D; the immediate moxibustion group was treated with eleventh d after injection of the uterus (after the success of the dysmenorrhea model replication), a total of the moxibustion group in advance was made in the moxibustion group in the model of advance moxibustion group in the model of advance moxibustion group in the model of advance moxibustion group in model making in advance moxibustion group in model in advance moxibustion group in model During the period of eighth D treatment and eleventh D for the end of the treatment, a total of 4 D.3 moxibustion groups were all with a diameter of 7 mm thickness, at the Shen que, Guan yuan point at the same time temperature and moxibustion, 10 min/ points, 1 /d. control group, the model group adopted the same body position with the moxibustion group, 10 min/ times, 1 /d. except the control group, the other 4 groups of cold wet stagnation model PD model rats. After the model was injected with oxytocin, the behavioral response was observed. After the end of the behavior test, the left uterus tissue of the "Y" shaped uterus was quickly separated on the ice plate after the end of the behavior test. 30% of the uterine tissue homogenate was made by adding physiological saline and milling with normal saline. At 4 centigrade of 3000 rpm centrifugation at low temperature. 15 min, taking the supernatant, using radioimmunoassay to detect the content of PGE2, AVP, ET-1 in the uterus tissue of rats. The content of PGF2 alpha in the uterus tissue of rats was detected by enzyme linked immunoassay. The content of NO in the uterus tissue of rats was detected by biochemical technique. The right uterus tissue was fixed with a concentration of 4% Formaldehyde Solution, paraffin embedding, section, and part of the immunization. The histochemical method was used to detect the MVD of the uterus tissue, the remaining part was used for the HE staining. The morphological changes of the MVD and the uterine tissue in the uterus were observed under the light microscope. Results: 1 the effects of moxibustion on the behavior of the cold wet and stagnant PD rats were observed: the prognosis of the moxibustion and the moxibustion group, the immediate moxibustion group, beforehand, The latent period of dysmenorrhea in the group of moxibustion was obviously prolonged, the number of twisting body decreased and the total score of torsional body decreased. The difference was statistically significant (P0.01) compared with the model group. Compared with the 3 moxibustion groups, the number of twisting bodies and the total twisting body score of the group of immediate moxibustion group and the pre moxibustion group in advance were significantly reduced and reduced (P0.01), and the immediate moxibustion group was in advance. Compared with the pre moxibustion group, the latent period of the dysmenorrhea was significantly prolonged (P0.01). Compared with the immediate moxibustion group, the latent period of the pre moxibustion group in advance was significantly longer than that in the immediate moxibustion group. The number of twisting bodies and the total twisting body score decreased and decreased significantly (P0.05 or P0.01). The moxibustion of the moxibustion group was not simultaneously moxibustion on the uterine tissue cells of the cold and wet PD rats. The effect of Pathomorphology on HE staining of uterus tissue was placed under the light microscope. General morphological observation showed that the morphology of uterus in the control group was regular, no edema and large number of neutrophils; the model group of rats had more regular tissue morphology, severe edema, hyperemia, large amount of neutrophils and lymphocytes; pre moxibustion group, immediate moxibustion group, pre moxibustion group, pre moxibustion group In the first instant moxibustion group, the tissues of the uterus of the rats were all morphologic rules, the tissue was slightly edema, no hyperemia, a large number of neutrophils, and a small amount of lymphocyte.3 did not simultaneously moxibustion on the MVD value of the uterus tissue of the cold wet and stagnant PD rats. The MVD of the uterus tissue of the rat model group was significantly reduced, compared with the control group, the difference was statistically significant (P0.05); moxibustion group in advance was in advance. In the instant moxibustion group, compared with the model group, the MVD of the uterus tissue of the 3 moxibustion groups was significantly increased, and the difference was statistically significant (P0.01). Compared with the pre moxibustion group in advance, the immediate moxibustion group was significantly more MVD than the pre moxibustion group (P0.05). The immediate moxibustion group was compared with the pre moxibustion group, and the immediate moxibustion group was more MVD than the pre moxibustion group. Although there were numerical differences, there was no statistical difference (P0.05). Compared with the immediate moxibustion group, the pre moxibustion group had the difference between the immediate moxibustion group and the immediate moxibustion group, but there was no statistical difference (P0.05) the effect of moxibustion on the plasma TXB2 and 6-keto-PGF1 alpha in the plasma of the cold wet and stagnant PD rats (.4) and the plasma TXB2 content and TXB2 in the model group of Dysmenorrhea Rats The ratio of /6-keto-PGF1 alpha was significantly increased, and the content of 6-keto-PGF1 a decreased significantly. Compared with the control group, the difference was statistically significant (P0.01). The prognosis of the moxibustion and moxibustion group, the immediate moxibustion group, the TXB2/6-keto-PGF1 alpha ratio of the plasma TXB2 and the TXB2/6-keto-PGF1 alpha in the pre moxibustion group were significantly decreased, the content of 6-keto-PGF1 alpha was significantly increased, and the model group was significantly higher. Compared, the difference was statistically significant (P0.01); compared with the pre moxibustion group, the plasma TXB2 content and TXB2/6-keto-PGF1 alpha ratio in the pre moxibustion group in advance were significantly lower than that in the pre moxibustion group, and the content of 6-keto-PGF1 a was significantly increased (P0.01 or P0.05); that is, the immediate moxibustion group was compared with the pre moxibustion group, and the immediate moxibustion group was more than the pre moxibustion group blood. There was no significant difference in plasma TXB2,6-keto-PGF1 alpha content and TXB2/6-keto-PGF1 alpha ratio, but there was no statistical significance (P0.05). Compared with immediate moxibustion group and immediate moxibustion group, the plasma TXB2 content and TXB2/6-keto-PGF1 alpha ratio of rats in the immediate moxibustion group were significantly lower than those in the immediate moxibustion group, and the content of 6-keto-PGF1 a significantly increased (P0.01).5 not simultaneously. The effect of moxibustion on the PGF2 alpha, PGE2 content and the ratio of PGF2 a /PGE2 in the uterus tissue of PD rats with cold and wet stagnation, the content of PGF2 alpha and the ratio of PGF2 alpha /PGE2 in the model group were significantly increased, the content of PGE2 decreased obviously. The difference was statistically significant (P0.01) compared with the control group (P0.01). The prognosis of moxibustion, moxibustion group, immediate moxibustion group, pre moxibustion group, precondition In the first instant moxibustion group, the content of PGF2 alpha and the ratio of PGF2 alpha /PGE2 were significantly decreased, and the content of PGE2 increased significantly. Compared with the model group, the difference was statistically significant (P0.01 or P0.05). Compared with the pre moxibustion group, the content of PGF2 A and the PGF2 a /PGE2 ratio of the uterus in advance moxibustion group were compared with those in the pre moxibustion group. Significantly lower (P0.01), the content of PGE2 increased significantly (P0.01). Compared with the pre moxibustion group, the content of PGF2 alpha and the ratio of PGF2 to /PGE2 in the immediate moxibustion group were significantly lower than those in the pre moxibustion group (P0.05) and the PGE2 content was significantly increased (P0.01). The advance moxibustion group was compared with the immediate moxibustion group, and the advance moxibustion group was compared with the engraved moxibustion group of the group of Dysmenorrhea Rats' uterus tissue PGF. The content of 2 alpha and the ratio of PGF2 alpha /PGE2 decreased significantly (P0.01), and the content of PGE2 increased significantly (P0.01).6. The effect of moxibustion on AVP content in the uterine tissue of cold wet and stagnant PD rats was significantly higher than that in the control group. The difference was statistically significant (P0.01) compared with the control group (P0.01). Moxibustion was a prognosis and moxibustion. Group, instant moxibustion group, in advance immediate moxibustion group 3 moxibustion groups of 3 groups of Dysmenorrhea Rats uterine tissue decreased significantly, and compared with the model group, the difference was statistically significant (P0.05 or P0.01). The pre moxibustion group, the immediate moxibustion group, the pre moxibustion group of 3 groups 22 compared, the 3 groups of uterine tissues, although there is a significant difference in AVP content, but no statistical significance (P0.05).7 is not The effect of moxibustion on ET-1 and NO content in uterus tissue of cold wet and stagnant PD rats increased significantly the content of ET-1 in uterus tissue of rats with dysmenorrhea, and the content of NO decreased significantly. Compared with the control group, the difference was statistically significant (P0.01). The prognosis of moxibustion and moxibustion, moxibustion group, immediate moxibustion group, and immediate moxibustion group of rat uterus tissue ET- The content of 1 was obviously decreased and the content of NO increased obviously. Compared with the model group, the difference was statistically significant (P0.01). Compared with the pre moxibustion group, the content of ET-1 in the pre moxibustion group in advance was significantly lower than that in the pre moxibustion group, and the content of NO increased significantly (P0.01). The immediate moxibustion group was compared with the pre moxibustion group, the immediate moxibustion group was compared with the pre moxibustion in the immediate moxibustion group. The content of ET-1 was obviously reduced, and the content of NO increased obviously (P0.05). Compared with the immediate moxibustion group, the content of ET-1 in the uterus tissue of the rats in advance moxibustion group was obviously lower than that in the immediate moxibustion group, and the content of NO was obviously increased (P0.05). Conclusion: 1 pre moxibustion, instant moxibustion and the intervention at different time of immediate moxibustion at different time can obviously reduce the cold dampness. The abdominal contraction and torsional body reaction in the stagnant PD rats obviously prolong the incubation period, but the effect is especially obvious in advance. It shows that moxibustion at different time has good analgesic effect on the rats of cold wet PD model, but the effect of immediate moxibustion with immediate immediate moxibustion can improve the cold dampness by three different kinds of moxibustion at different time. The uterine tissue congestion and edema state of the PD model rats, but there is no obvious difference between the 3 groups of pre moxibustion, immediate moxibustion and pre moxibustion,.3 three different time moxibustion can increase the MVD of the uterus tissue of the cold wet and stagnant PD model rats, which can improve the local microcirculation of the uterus. The effect of the first instant moxibustion group is the best, obviously better than the pre moxibustion and instant moxibustion. The effect may be through the warm effect of the fire, the heat transfer of the meridians and the transmission of nerve to the uterus, in order to increase the blood flow, change the local blood circulation, and then to alleviate the pain,.64 can reduce the cold wet PD mold with three different kinds of moxibustion. The content of plasma TXB2 and the ratio of TXB2/6-keto-PGF1 a, and the increase of the content of 6-keto-PGF1 a, especially the immediate moxibustion in advance, showed that moxibustion at different times had a good regulating effect on the above indexes, but the effect of immediate moxibustion was more significant. The mechanism of its action may be by regulating the activity of platelet. And vascular tension, improve the state of vasoconstriction, increase the local blood flow of the uterus, and achieve the purpose of relieving pain..5 three different moxibustion at different time can reduce the content of PGF2 alpha and the ratio of PGF2 a /PGE2 in the uterus tissue of PD model rats with cold damp and stagnation, and improve the level of PGE2, but the effect is especially obvious in advance moxibustion. At the same time, moxibustion has a good regulating effect on the abnormal level of PGF2 alpha and PGE2, and the effect of immediate moxibustion is more significant. Its mechanism may be to improve the endocrine environment of the body by restoring the balance of PGF2 alpha and PGE2, and then to inhibit the uterine smooth muscle and vascular spasmodic contraction and improve the local ischemic and anoxic state. Three different moxibustion at different times of.6 can obviously reduce the AVP content in the uterus tissue of PD model rats with cold dampness and stagnation, and there is no obvious difference between them. The mechanism may be to reduce the synthesis and release of PG by reducing the content of AVP, thus effectively inhibiting the spastic contraction of the uterine smooth muscle and improving the local blood circulation. Increase the blood flow, alleviate the ischemic state, and play its analgesic effect,.7 three different time moxibustion can reduce the content of ET-1 and increase the content of NO, in which the immediate moxibustion is the most obvious, the immediate moxibustion is the second. The mechanism may be by relaxing the uterine smooth muscle, inhibiting its contraction, improving the uterine tissue microcirculation and increasing its blood flow.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R245

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