本文選題：電針 + 胃經(jīng)穴��； 參考：《中華中醫(yī)藥雜志》2017年03期

【摘要】：目的:采用核磁共振氫譜技術(shù)(~1H NMR)研究電針胃經(jīng)穴對應(yīng)激性胃潰瘍大鼠胃黏膜組織代謝物譜的表達。方法:40只SD大鼠隨機分為正常組、模型組、胃經(jīng)組和膽經(jīng)組。利用束縛-冷應(yīng)激法制備應(yīng)激性胃潰瘍模型大鼠,光鏡下觀察大鼠胃黏膜組織病理形態(tài)學(xué)的變化,采用~1H NMR技術(shù)獲取胃黏膜組織~1H NMR譜,并利用模式識別方法分析各組間的代謝物譜的差異。結(jié)果:與正常組比較,模型組大鼠胃黏膜損傷和胃黏膜組織代謝輪廓差異明顯,胃黏膜組織乙酸、肌醇、磷酸膽堿、谷氨酸、甘氨酸、乙醇胺、賴氨酸、3-羥基丁酸、纈氨酸、異亮氨酸、�；撬�、亮氨酸和甘露醇含量上升(P0.05),乳酸、甲硫氨酸和天冬氨酸含量下降(P0.05);與模型組比較,胃經(jīng)組和膽經(jīng)組大鼠胃黏膜得到明顯修復(fù),胃經(jīng)組大鼠胃黏膜組織代謝輪廓更接近正常大鼠,胃黏膜組織甲硫氨酸、天冬氨酸含量上升(P0.05),乙酸、肌醇、磷酸膽堿、谷氨酸、甘氨酸、賴氨酸、異亮氨酸、�；撬�、乳酸、亮氨酸和甘露醇含量下降(P0.05);膽經(jīng)組大鼠胃黏膜組織3-羥基丁酸、甲硫氨酸含量上升(P0.05),肌醇、磷酸膽堿、谷氨酸、乙醇胺、異亮氨酸、乳酸、亮氨酸和甘露醇含量下降(P0.05)。結(jié)論:電針胃經(jīng)穴可調(diào)節(jié)胃潰瘍大鼠胃黏膜組織特異代謝物的表達,促進胃黏膜損傷的修復(fù)。
[Abstract]:Aim: to study the expression of metabolites in gastric mucosa of stress gastric ulcer rats by electroacupuncture at gastric meridian by 1H NMR.Methods 40 Sprague-Dawley rats were randomly divided into normal group, model group, gastric meridian group and gallbladder meridian group.The gastric ulcer model rats were prepared by the method of restraint and cold stress. The histopathologic changes of gastric mucosa were observed under light microscope. The 1H NMR spectra of gastric mucosa were obtained by 1H NMR technique.The difference of metabolite spectrum was analyzed by pattern recognition method.Results: compared with the normal group, the gastric mucosal injury and the metabolic profile of the gastric mucosa in the model group were significantly different. The gastric mucosal tissues were acetic acid, inositol, choline phosphate, glutamate, glycine, ethanolamine, lysine 3-hydroxybutyric acid, valine.The contents of isoleucine, taurine, leucine and mannitol increased, while the contents of lactic acid, methionine and aspartic acid decreased, compared with the model group, the gastric mucosa was significantly repaired in the gastric meridian group and the gallbladder meridian group.The metabolic profile of gastric mucosa in gastric meridian group was more similar to that in normal rats. The contents of methionine and aspartic acid in gastric mucosa were increased (P 0.05), acetic acid, inositol, choline phosphate, glutamate, glycine, lysine, isoleucine, taurine, lactic acid, glutamate, glycine, lysine, isoleucine, taurine, lactic acid,The contents of 3-hydroxybutyric acid and methionine in gastric mucosa of rats in the gallbladder meridian group were increased, while the contents of inositol, choline phosphate, glutamate, ethanolamine, isoleucine, lactic acid, leucine and mannitol were decreased.Conclusion: EA can regulate the expression of specific metabolites in gastric mucosa and promote the repair of gastric mucosal injury.
【作者單位】： 廈門大學(xué)醫(yī)學(xué)院中醫(yī)系;廈門大學(xué)深圳研究院;湖南中醫(yī)藥大學(xué)針灸推拿學(xué)院;
【基金】：國家自然科學(xué)基金項目(No.81473751,No.81260556) 國家重點基礎(chǔ)研究發(fā)展計劃(973計劃)項目(No.2015CB554502) 廣東省自然科學(xué)基金項目(No.2014A030313005) 深圳市科技計劃項目(No.JCYJ20160406140612883)~~
【分類號】：R245.97

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