本文關(guān)鍵詞：經(jīng)筋微創(chuàng)松解療法對(duì)膝骨關(guān)節(jié)炎兔軟骨細(xì)胞凋亡的抑制作用及機(jī)制　出處：《山東醫(yī)藥》2017年21期 　論文類型：期刊論文

  更多相關(guān)文章： 膝骨關(guān)節(jié)炎 經(jīng)筋微創(chuàng)松解療法 中醫(yī) 細(xì)胞凋亡 細(xì)胞炎性因子 MAPKs信號(hào)通路

【摘要】：目的觀察經(jīng)筋微創(chuàng)松解療法對(duì)膝骨關(guān)節(jié)炎兔軟骨細(xì)胞凋亡的抑制作用,并探討其作用機(jī)制。方法將30只健康白兔隨機(jī)均分為正常組、模型組與治療組。模型組和治療組采用膝關(guān)節(jié)制動(dòng)法制備關(guān)節(jié)炎動(dòng)物模型。治療組給予經(jīng)筋微創(chuàng)松解療法治療4周,其余兩組不做處理。耳緣靜脈空氣栓塞法處死實(shí)驗(yàn)動(dòng)物,取其脛骨軟骨組織及膝關(guān)節(jié)液。用TUNEL細(xì)胞凋亡檢測法測算膝關(guān)節(jié)軟骨細(xì)胞凋亡指數(shù),用ELISA法檢測各組膝關(guān)節(jié)液中白細(xì)胞介素1β(IL-1β)、轉(zhuǎn)化生長因子β1(TGF-β1)和腫瘤壞死因子α(TNF-α),Western blotting技術(shù)檢測膝關(guān)節(jié)軟骨組織中氨基末端激酶(JNK)和細(xì)胞外信號(hào)調(diào)節(jié)激酶(ERK)蛋白表達(dá)。結(jié)果模型組膝關(guān)節(jié)軟骨細(xì)胞凋亡指數(shù)及膝關(guān)節(jié)液中IL-1β、TNF-α、TGF-β1水平,ERK、JNK蛋白相對(duì)表達(dá)量與對(duì)照組比較,P均0.05;治療組軟骨細(xì)胞凋亡指數(shù)及膝關(guān)節(jié)液中IL-1β、TNF-α、TGF-β1水平,ERK、JNK蛋白相對(duì)表達(dá)量與模型組比較,P均0.05。結(jié)論經(jīng)筋微創(chuàng)松解療法可抑制膝骨關(guān)節(jié)炎兔軟骨細(xì)胞凋亡,其作用機(jī)制可能為降低關(guān)節(jié)液中炎性因子水平及軟骨細(xì)胞MAPKs信號(hào)通路相關(guān)蛋白表達(dá)。
[Abstract]:Objective to observe the inhibitory effect of minimally invasive release therapy of meridian on chondrocyte apoptosis in rabbits with knee osteoarthritis and its mechanism. Methods 30 healthy rabbits were randomly divided into normal group. The model group and the treatment group. The model group and the treatment group using the knee joint immobilization method to make arthritis animal model, the treatment group was given meridian minimally invasive release therapy for 4 weeks. The other two groups were not treated. The experimental animals were killed by auricular vein air embolization. The tibial cartilage tissue and knee joint fluid were taken. The apoptosis index of chondrocytes of knee joint was measured by TUNEL cell apoptosis assay. The levels of IL-1 尾, TGF- 尾 1, TGF- 尾 1 and TNF- 偽 in the knee joint fluid of each group were detected by ELISA method. Detection of amino-terminal kinase (Western) and extracellular signal-regulated kinase (ERK) in articular cartilage by Western blotting. Results the apoptotic index of chondrocytes and IL-1 尾 in knee joint fluid in the model group. Compared with the control group, the relative expression of TNF- 偽 TGF- 尾 1 and ERKN JNK protein were 0.05; The apoptotic index of chondrocytes and the relative expression of IL-1 尾 -TNF- 偽 TGF- 尾 1 in the treatment group were compared with those in the model group. Conclusion minimally invasive release therapy of meridians can inhibit chondrocyte apoptosis in rabbits with knee osteoarthritis. The mechanism may be to reduce the level of inflammatory factors in articular fluid and the expression of MAPKs signaling pathway related protein in chondrocytes.
【作者單位】： 廣州軍區(qū)廣州總醫(yī)院;廣州中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金資助項(xiàng)目(815738831008646) 廣東省科技計(jì)劃項(xiàng)目應(yīng)用型科技研發(fā)專項(xiàng)(2015B02033011)
【分類號(hào)】：R274
【正文快照】： 膝骨關(guān)節(jié)炎是一種關(guān)節(jié)軟骨慢性退變導(dǎo)致的骨關(guān)節(jié)疾病,多發(fā)于中老年人,臨床主要以軟骨退變和滑膜炎癥為特征[1]。性別、體質(zhì)量、年齡、遺傳等全身因素和關(guān)節(jié)力學(xué)、細(xì)胞炎癥或凋亡因子、軟骨細(xì)胞凋亡以及降解酶等局部因素綜合作用,導(dǎo)致骨關(guān)節(jié)軟骨發(fā)生代謝、生化環(huán)境和結(jié)構(gòu)改變,

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊國晶;朱慧明;;循經(jīng)推運(yùn)松解療法的臨床應(yīng)用[J];吉林中醫(yī)藥;1991年03期

2 羅濤;劉景;;微創(chuàng)松解療法治療指屈肌腱狹窄性腱鞘炎研究[J];中國骨傷;2013年05期

3 趙亞男;劉紅星;楊旭東;馮家云;李春苗;;中藥沖擊松解療法治療青少年頸椎病的臨床觀察[J];河北中醫(yī)藥學(xué)報(bào);2010年01期

4 王小軍;彭南彩;;土家醫(yī)傳統(tǒng)松解療法治療肩周炎80例臨床觀察[J];中國民族醫(yī)藥雜志;2011年06期

5 楊永淑;循經(jīng)推運(yùn)松解療法簡介[J];冶金醫(yī)藥情報(bào);1989年05期

6 鄧成勇;周全;;小針刀松解療法治療跟骨骨質(zhì)增生臨床觀察[J];中醫(yī)學(xué)報(bào);2013年09期

7 王曉晨;;循經(jīng)推運(yùn)松解療法治療急性化膿性乳腺炎48例觀察[J];中國社區(qū)醫(yī)師;1990年12期

8 劉俊偉;;錘擊松解療法可治足跟痛[J];現(xiàn)代養(yǎng)生;2011年17期

9 楊慎峭;;論針刀松解療法的治療作用[J];貴陽中醫(yī)學(xué)院學(xué)報(bào);2012年01期

10 章瑛,李家邦,周錦財(cái),周中煥;針刀松解療法引起頸段脊髓刺激癥1例報(bào)告[J];頸腰痛雜志;2004年06期

相關(guān)會(huì)議論文 前1條

1 章瑛;董�；�;;跟痛癥的發(fā)病機(jī)理及閉合型松解療法的臨床研究[A];中華中醫(yī)藥學(xué)會(huì)針刀醫(yī)學(xué)分會(huì)二00七年度學(xué)術(shù)年會(huì)論文集[C];2007年

，

本文編號(hào)：1430054