【摘要】：目的腫瘤源性的血管性血友病因子(von Willbrend factor,vWF)表達(dá)及血管生成擬態(tài)(vasculogenic mimicry,VM)形成都是高度惡性腫瘤中的高頻事件。本研究檢測(cè)甲狀腺未分化癌組織中vWF的表達(dá)及VM水平,在組織學(xué)水平上探討vWF的表達(dá)及其與腫瘤組織中VM形成的相關(guān)性。方法選取甘肅省人民醫(yī)院(20例)及甘肅省腫瘤醫(yī)院(43例)2000-01-01-2014-12-31病理診斷為甲狀腺未分化癌的手術(shù)標(biāo)本63例。同時(shí)隨機(jī)挑選甘肅省人民醫(yī)院同期甲狀腺乳頭狀癌病例63例和相應(yīng)癌旁正常組織作為對(duì)照。采用免疫組化法檢測(cè)甲狀腺組織中vWF蛋白的表達(dá)水平,CD31聯(lián)合PAS雙重染色檢測(cè)VM水平。結(jié)果vWF蛋白在正常甲狀腺組織無表達(dá),甲狀腺乳頭狀癌中有5例(7.90%)陽性表達(dá),甲狀腺未分化癌中vWF陽性表達(dá)31例(49.20%),組間比較差異有統(tǒng)計(jì)學(xué)意義,χ~2=26.289,P0.001。vWF在甲狀腺未分化癌中的陽性表達(dá)與患者的性別、年齡、腫瘤大小和淋巴結(jié)轉(zhuǎn)移無關(guān),而與遠(yuǎn)處轉(zhuǎn)移(χ~2=5.341,P=0.021)和臨床分期(χ~2=4.753,P=0.029)相關(guān)。VM在正常甲狀腺組織、乳頭狀癌及甲狀腺未分化癌中的形成率分別為0、3.20%和34.92%,組間差異有統(tǒng)計(jì)學(xué)意義,χ~2=20.588,P0.001。VM形成與腫瘤最大徑(χ~2=4.789,P=0.029)、淋巴結(jié)轉(zhuǎn)移(χ~2=5.684,P=0.028)、遠(yuǎn)處轉(zhuǎn)移(χ~2=4.722,P=0.030)和臨床分期(χ~2=5.777,P=0.061)相關(guān)。vWF的表達(dá)與VM水平正相關(guān),r=0.411,P=0.001。結(jié)論甲狀腺未分化癌中有不同程度vWF的高表達(dá),其高表達(dá)與VM的形成正相關(guān),vWF的高表達(dá)可能是導(dǎo)致甲狀腺未分化癌預(yù)后差的因素之一。
[Abstract]:Objective the expression of tumor derived von Willebrand factor (von Willbrend factor,vWF) and the formation of angiogenic mimicry (vasculogenic mimicry,VM) are high frequency events in highly malignant tumors. In this study, the expression of vWF and the level of VM were detected in undifferentiated thyroid carcinoma. The expression of vWF and its correlation with the formation of VM in tumor tissue were studied at the histological level. Methods Sixty-three cases of undifferentiated thyroid carcinoma were selected from Gansu Provincial people's Hospital (20 cases) and Gansu Cancer Hospital (43 cases) from January to January 2014-12-31, 2000. At the same time, 63 cases of thyroid papillary carcinoma in Gansu Provincial people's Hospital were randomly selected as control group. The expression of vWF protein in thyroid tissue was detected by immunohistochemical method and the VM level was detected by double staining of CD31 and PAS. Results there was no expression of vWF protein in normal thyroid tissue, and 5 cases (7.90%) were positive in papillary thyroid carcinoma. 31 cases (49.20%) of thyroid undifferentiated carcinoma showed vWF positive expression. The positive expression of vWF in undifferentiated thyroid carcinoma was not correlated with sex, age, tumor size and lymph node metastasis. VM was associated with distant metastasis (蠂 2 + 5.341) and clinical stage (蠂 2 / 24.753) in normal thyroid tissue. The formation rates of papillary carcinoma and thyroid undifferentiated carcinoma were 0 / 3.20% and 34.92%, respectively. There was significant difference between the two groups. The expression of VWF was positively correlated with the VM level and the maximum diameter of the tumor (蠂 2 / 24.789 / P 0.029), lymph node metastasis (蠂 ~ 2 / 25.684P / 0.028), distant metastasis (蠂 ~ 2 / 2 / 4.722 / P ~ 0.030) and clinical stage (蠂 ~ 25.777A / P 0.061). The expression of VWF was positively correlated with the VM level. Conclusion there is high expression of vWF in undifferentiated thyroid carcinoma. The high expression of vWF and the formation of VM may be one of the factors leading to poor prognosis of undifferentiated thyroid carcinoma.
【作者單位】： 甘肅省人民醫(yī)院病理科;甘肅省腫瘤醫(yī)院病理科;
【分類號(hào)】：R736.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 徐德忻,王筱芬;1004例住院肺癌患者致病因素分析[J];山東醫(yī)藥;1986年05期

2 黃裕新,許才紱,張少玲;胃未分化癌30例臨床觀察[J];第四軍醫(yī)大學(xué)學(xué)報(bào);1993年03期

3 袁靜，金景澍;207例肺癌纖維支氣管鏡檢查特點(diǎn)及其臨床意義[J];齊魯腫瘤雜志;1995年04期

4 種道群;賁門未分化癌心包轉(zhuǎn)移一例[J];河南腫瘤學(xué)雜志;1996年05期

5 白信花;郝彥勇;王帥;武艷;;甲狀腺未分化癌的臨床病理分析[J];吉林醫(yī)學(xué);2008年22期

6 Deal,C.W;魏大藻;;肺切除術(shù)后支氣管雀麥細(xì)胞癌與未分化癌預(yù)后之比較[J];廣東醫(yī)學(xué);1964年S6期

7 江巨釧;胃未分化癌1例[J];實(shí)用兒科臨床雜志;1995年04期

8 劉釗,艾冬華,趙華妹;1例食道未分化癌的誤診分析[J];海軍醫(yī)學(xué)雜志;2003年02期

9 張志賢,谷銑之,殷蔚伯,苗延俊;食管未分化癌的治療[J];中國放射腫瘤學(xué);1991年02期

10 丁家增,蔡偉耀,楊衛(wèi)平,陳曦,嚴(yán)佶祺,李宏為;甲狀腺未分化癌的處理及影響預(yù)后因素的探討[J];中國腫瘤臨床;2002年09期

相關(guān)會(huì)議論文 前1條

1 黃潤生;劉英姿;吳秀花;蔣莉莉;;甲狀腺未分化癌超聲表現(xiàn)一例[A];中華醫(yī)學(xué)會(huì)第十三次全國超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2013年

相關(guān)碩士學(xué)位論文 前2條

1 劉楊;自噬對(duì)甲狀腺未分化癌發(fā)病機(jī)制及放化療增敏作用的研究[D];吉林大學(xué);2015年

2 韓若闊;甲狀腺未分化癌12例臨床分析并文獻(xiàn)復(fù)習(xí)[D];浙江大學(xué);2009年

，

本文編號(hào)：2254761