發(fā)布時間：2018-05-22 19:21

  本文選題：癌 + 肝細胞��； 參考：《中國普通外科雜志》2017年07期

【摘要】：目的:探討miR-96在肝細胞癌(HCC)細胞中的表達及作用。方法:用qRT-PCR測定miR-96在不同HCC細胞系(HepG2、7721、huh7)及正常肝細胞系L02中的表達;將HepG2細胞分別轉染miRNA隨機序列(陰性對照組)、miR-96模擬物(miR-96模擬物組)和miR-96抑制物(miR-96抑制物組)后,用細胞劃痕實驗及Transwell細胞侵襲實驗分別檢測細胞遷移及侵襲能力,qRT-PCR及Western blot分別測定PTPN9 mRNA及蛋白的表達。結果:miR-96在各肝癌細胞系中的相對表達量均明顯高于其在正常肝細胞系L02的相對表達量(均P0.01)。與陰性對照組比較,細胞劃痕愈合率在miR-96模擬物組明顯升高,而在miR-96抑制物組明顯降低(均P0.05);侵襲細胞數(shù)在miR-96模擬物組明顯增多,而在miR-96抑制物組明顯減少(均P0.05);PTPN9 mRNA與蛋白相對表達量在miR-96模擬物組均明顯下調(diào),而在miR-96抑制物組均明顯上調(diào)(均P0.05)。結論:miR-96在HCC細胞中表達升高,并可能通過下調(diào)PTPN9表達促進HCC細胞的遷移與侵襲。
[Abstract]:Objective: To investigate the expression and role of miR-96 in hepatocellular carcinoma (HCC) cells. Methods: the expression of miR-96 in different HCC cell lines (HepG27721, Huh7) and normal liver cell line L02 was detected by qRT-PCR, and HepG2 cells were transfected into miRNA random sequence (negative control group), miR-96 analogue (miR-96 analogue group) and inhibitor inhibitor. The cell migration and invasion ability were detected by cell scratch test and Transwell cell invasion test, and the expression of PTPN9 mRNA and protein were measured by qRT-PCR and Western blot respectively. Results: the relative expression of miR-96 in all hepatoma cell lines was significantly higher than that of its relative expression in normal liver cell line L02 (P0.01). Compared with the sex control group, the scar healing rate was significantly increased in the miR-96 simulation group, but in the miR-96 inhibitor group (all P0.05), the number of invasive cells in the miR-96 simulation group was significantly increased, while the miR-96 inhibitor group decreased significantly (P0.05), and the relative expression of PTPN9 mRNA and protein decreased significantly in the miR-96 analog group, but in miR-9. 6 the inhibitor group was significantly up-regulated (all P0.05). Conclusion: miR-96 expression is increased in HCC cells, and may promote the migration and invasion of HCC cells by down regulating PTPN9 expression.
【作者單位】： 華中科技大學同濟醫(yī)學院附屬武漢中心醫(yī)院肝膽胰外科;
【分類號】：R735.7

【參考文獻】

相關期刊論文 前6條

1 張紅紅;劉曉麗;許思娟;;宮頸癌組織中miRNA-34c的表達水平分析及其靶基因PLK4的鑒定[J];中國腫瘤臨床;2015年22期

2 鞠紅艷;白洋;王策;;轉化生長因子β激活酶1在結腸癌中的表達及臨床意義[J];中國普通外科雜志;2015年10期

3 徐泰;;miRNA-639在乳腺癌中表達及其意義[J];中國普通外科雜志;2014年11期

4 宋世鐸;周健;何宋兵;朱東明;趙華;張子祥;王毅;李德春;;miRNA-375對胰腺癌細胞Panc-1增殖和凋亡的影響[J];中國腫瘤臨床;2013年01期

5 施明亮;陳勇;劉巍;;miR-21與消化系統(tǒng)腫瘤[J];中國腫瘤臨床;2011年24期

6 阮永威;田興松;侯連澤;;乳腺癌p16,p53基因蛋白和mRNA的表達及其意義[J];中國普通外科雜志;2008年05期

【相似文獻】

相關期刊論文 前10條

1 王拱辰;施廣霞;;肝細胞癌細胞免疫功能的相關研究[J];中國肝臟病雜志(電子版);2010年02期

2 張永杰,吳孟超;肝細胞癌細胞核DNA流式細胞術分析[J];中國腫瘤臨床;1994年03期

3 龔祖元;廖彩仙;王宇;廖欣鑫;秦安成;黃勇平;廖暉;;肝細胞癌細胞膜表面唾液酸含量的變化[J];南方醫(yī)科大學學報;2010年10期

4 楊連君,隋延仿,陳志南,徐力青,米力,曲萍,劉成鋼;HLA-DR抗原在培養(yǎng)的肝細胞癌細胞表面的表達及意義[J];中國腫瘤生物治療雜志;1997年03期

5 戴越盟,林琦遠,嚴律南,林萍,智星,雷松,屈藝;阿霉素誘導人肝細胞癌細胞SMMC-7721耐藥性的產(chǎn)生及其機理的研究[J];中華腫瘤雜志;2000年01期

6 張海增,林晨,邵永孚,隗月,余昌中,付明,張雪艷,梁肖,吳e，

本文編號：1923305