發(fā)布時(shí)間：2018-01-11 08:24

  本文關(guān)鍵詞：白介素-33在結(jié)直腸癌中表達(dá)及臨床意義　出處：《蘇州大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 白細(xì)胞介素-33 結(jié)直腸癌 臨床意義

【摘要】：目的探討結(jié)直腸癌組織中白介素-33(interleukin-33,IL-33)表達(dá)與臨床特征關(guān)系,并分析其與患者預(yù)后關(guān)系。研究結(jié)直腸癌患者血清可溶性白介素-33(soluble interleukin-33,sIL-33)水平與臨床特征關(guān)系,評(píng)價(jià)其對(duì)結(jié)直腸癌診斷意義。方法結(jié)直腸癌組織芯片中包含98例癌組織和80例癌旁組織。采用免疫組織化學(xué)染色法檢測(cè)其中IL-33表達(dá),采用卡方檢驗(yàn)比較結(jié)直腸癌和癌旁組織中IL-33陽(yáng)性表達(dá)比例差異,分析不同臨床特征患者IL-33表達(dá)差異,采用Kaplan-Meier法分析IL-33表達(dá)與患者總生存期(Overall survival,OS)關(guān)系,并擬合Cox比例風(fēng)險(xiǎn)模型。收集121例結(jié)直腸癌患者術(shù)前血清與臨床特征資料,并收集33例腸息肉患者和81例健康體檢者作為對(duì)照。ELISA法檢測(cè)血清中sIL-33水平,分析其與臨床特征關(guān)系,采用ROC曲線、Logistic回歸模型分析sIL-33、CEA和CA19-9三者單獨(dú)或聯(lián)合檢測(cè)對(duì)結(jié)直腸癌診斷價(jià)值。結(jié)果結(jié)直腸癌組織中IL-33陽(yáng)性表達(dá)率(38.78%)低于癌旁組織(63.75%),差異有統(tǒng)計(jì)學(xué)意義(P0.01)。IL-33表達(dá)與病理分級(jí)有關(guān),隨病理分級(jí)增加,陽(yáng)性率逐漸降低(I、II和III級(jí)分別為66.67%、36.76%和20%,P=0.027)。Kaplan-Meier法分析表明IL-33陽(yáng)性較陰性患者OS差異無(wú)統(tǒng)計(jì)學(xué)意義(log-rankχ2=0.3755,P=0.54)。多因素Cox比例風(fēng)險(xiǎn)模型表明,遠(yuǎn)處轉(zhuǎn)移(HR=7.841,P=0.002)和較晚的TNM分期(HR=2.594,P=0.003)為結(jié)直腸癌獨(dú)立預(yù)后因素。結(jié)直腸癌患者血清sIL-33含量172.44(108.08,321.57)pg/mL顯著高于腸息肉組67.31(37.72,130.44)pg/mL和健康體檢組71.73(36.80,85.53)pg/mL,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。sIL-33水平與結(jié)直腸癌分化程度、腫瘤部位有關(guān),差異有統(tǒng)計(jì)學(xué)意義(P0.01)。sIL-33、CEA和CA19-9三者聯(lián)合檢測(cè)的敏感性(80.99%)、準(zhǔn)確率(86.38%)和ROC曲線下面積(0.9334)均比單獨(dú)檢測(cè)高,其特異性(92.11%)也處于較高水平,表明具有較好的診斷價(jià)值。結(jié)論結(jié)直腸癌患者組織中IL-33陽(yáng)性表達(dá)率低于癌旁組織,且與病理分級(jí)有關(guān)。結(jié)直腸癌患者血清sIL-33含量顯著高于對(duì)照組,并且與分化程度、腫瘤部位有關(guān)。sIL-33、CEA和CA19-9三者聯(lián)合檢測(cè)對(duì)結(jié)直腸癌具有較高輔助診斷價(jià)值,可作為潛在的血清生物標(biāo)志物。
[Abstract]:Objective to investigate the relationship between interleukin-33 (IL-33) expression and clinical features in colorectal cancer. To study serum soluble interleukin-33 soluble interleukin-33 in patients with colorectal cancer. The level of sIL-33 was correlated with clinical features. Methods 98 cases of colorectal cancer and 80 cases of adjacent tissues were included in the tissue microarray. Immunohistochemical staining was used to detect the expression of IL-33. The difference of IL-33 positive expression in colorectal cancer and paracancerous tissues was compared by chi-square test, and the difference of IL-33 expression in patients with different clinical characteristics was analyzed. The relationship between the expression of IL-33 and the total survival time of patients was analyzed by Kaplan-Meier method. Cox proportional risk model was fitted to collect data of serum and clinical features of 121 patients with colorectal cancer before operation. The serum sIL-33 levels were measured by Elisa in 33 patients with intestinal polyps and 81 healthy controls. The relationship between the serum levels and clinical characteristics was analyzed and ROC curve was used. Logistic regression model was used to analyze sIL-33. Results the positive expression rate of IL-33 in colorectal cancer tissues was 38.78), which was lower than that in paracancerous tissues (63.75%). . The expression of IL-33 was related to the pathological grade. With the increase of pathological grade, the positive rate decreased gradually, and the positive rate was 66.67% in grade 鈪，

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