發(fā)布時(shí)間：2021-08-11 05:44

　　目前醫(yī)學(xué)研究人員面臨的最迫切問(wèn)題之一是肥胖人群數(shù)量的不斷增加以及由肥胖引發(fā)的糖尿病和心血管綜合征（Syndrome）群體的不斷升高。高脂肪和高糖飲食,加上缺少運(yùn)動(dòng)的生活方式,是世界上肥胖增多的主要原因。肥胖導(dǎo)致的心血管疾病日益增多,非藥物治療代謝綜合征法包括良好的飲食,合理的體重維持,規(guī)律的鍛煉和科學(xué)的膳食補(bǔ)充劑。眾所周知,運(yùn)動(dòng)訓(xùn)練可降低幾種與年齡有關(guān)的疾病發(fā)病率,包括心血管疾病和Ⅱ型糖尿病。此外,運(yùn)動(dòng)訓(xùn)練是一種實(shí)用有效,非侵入性的干預(yù)措施,它對(duì)疾病的防治人類具有重要的作用。傳統(tǒng)中藥冬蟲夏草具有對(duì)中樞神經(jīng)系統(tǒng)有鎮(zhèn)靜、抗驚厥、降溫等作用,有抗腫瘤,抗疲勞,對(duì)體液免疫功能有增強(qiáng)作用。此外,冬蟲夏草可提高人類心臟耐缺氧能力,降低心臟對(duì)氧的消耗,抗心律失常,可以降低血液中的膽固醇和甘油三酯,提高對(duì)人體有利的高密度脂蛋白,減輕動(dòng)脈粥樣硬化,然而其中機(jī)制還不是很清楚。心臟收縮泵血功能正常與鈣離子信號(hào)調(diào)控密切相關(guān),其中CAMRA、Lamin C、PPTAC、CaMKⅡ和SERCA等鈣離子相關(guān)蛋白對(duì)心臟功能具有保護(hù)作用。PI3K/Akt/mTOR信號(hào)通路可感受并結(jié)合不同的營(yíng)養(yǎng)因素和環(huán)境因素,包括生長(zhǎng)因... 

【文章來(lái)源】：陜西師范大學(xué)陜西省 211工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：159 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
Abstract
Chapter Ⅰ Review of the factors that affect cardiac performance and the role of relevant genes
    1.1 Conservation of Drosophila and mammalian CAMRs
        1.1.1 The role of CAMRs in the nervous system
        1.1.2 Involvement of CAMRs in the Notch signaling pathways
        1.1.3 Role of CAMRs in cell viability and piRNA pathway
        1.1.4 Association between RyRs, TRP/TRPL and CAMRs
    1.2 Other relevant cardiac related genes
        1.2.1 The role of PPTA gene in cardiac performance
        1.2.2 The function of CaMKⅡ gene in cardiac performance
        1.2.3 The SERCA gene and cardiac function
        1.2.4 The role of Lamin C in cardiomyopathy
        1.2.5 Integrin and cardiac function
        1.2.6 The effect of Delta in cardiac function
    1.3 Effect of Drosophila melanogaster endurance training assays on cardiac performance
        1.3.1 Effect of exercise training on cardiovascular disease
        1.3.2 Endurance training assays for Drosophila melanogaster
    1.4 Fat and sugar metabolism in mammals and Drosophila
        1.4.1 Excess fat and sugar in Drosophila causes diabetic cardiomyopathy-like symptoms
        1.4.2 Effect of high-dat diet (HFD) on Drosophila heart function
        1.4.3 Effect of high-sugar diet (HSD) on Drosophila heart function
        1.4.4 Induced-obesity
    1.5 Mild stress
    1.6 Starvation resistance
    1.7 The uses of Cordyceps militaris
    1.8 The purpose and significance of the study
Chapter Ⅱ Bioinformatics analysis of calcium and cardiac related proteins
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Data-mining, protein structure and functional prediction
        2.2.2 Analysis of conserved motifs
        2.2.3 Construction of the PPI network
    2.3 Results
    2.4 Discussion
    2.5 Conclusion
Chapter Ⅲ Cordyceps improves cardiac performance and aging in endurance training Drosophila melanogaster
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Fly maintenance and collection
        3.2.2 Drug administration
        3.2.3 Mortality assay
        3.2.4 Lifespan assay for wild type flies
        3.2.5 Negative geotaxis assay
        3.2.6 Larval crawling assay
        3.2.7 Cold stress
        3.2.8 RNA extractions and RT-PCR
        3.2.9 qRT-PCR
        3.2.10 Total protein measurement
        3.2.11 Western blot analysis
        3.2.12 Courtship and mating
        3.2.13 Statistical analysis
    3.3 Results
        3.3.1 The role of Cordyceps and exercise in aging and cardiac function
        3.3.2 Effect of exercise and Cordyceps on Gene and protein expression
        3.3.3 Cordyceps does not improve mating and courtship
    3.4 Discussion
    3.5 Conclusion
Chapter Ⅳ The effect of induced obesity and Cordyceps militaris in Drosophila melanogaster cardiac performance and aging
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Fly rearing and collection
        4.2.2 HFD and HSD feeding regime
        4.2.3 Drug administration
        4.2.4 Lifespan assay for different fly strains
        4.2.5 Negative geotaxis assay for different fly strains
        4.2.6 Triglyceride, protein and glucose measurements
        4.2.7 Cold stress
        4.2.8 RNA extractions and RT-PCR
        4.2.9 qRT-PCR
        4.2.10 Western blot analysis
        4.2.11 Statistical analysis
    4.3 Results
        4.3.1 The effect of Cordyceps militaris in longevity and cardiac function of mutant flies
        4.3.2 Phenotypic profiles of flies fed with high-fat and high-sugar diet
        4.3.3 Effect of lipogenic diet on gene and protein expression
    4.4 Discussion
    4.5 Conclusion
Chapter Ⅴ Effect of combined stresses on Drosophila cardiac performance and mobility
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Fly rearing and collection
        5.2.2 Starvation resistance assay
        5.2.3 Negative geotaxis assay
        5.2.4 Cold pretreatment
        5.2.5 Cold stress
        5.2.6 Triglyceride, protein and glucose measurements
        5.2.7 RNA extractions and RT-PCR
        5.2.8 qRT-PCR
        5.2.9 Western blot analysis
        5.2.10 Statistical analysis
    5.3 Results
        5.3.1 The effect of mild stresses in cardiac function of flies
        5.3.2 The effect of starvation resistance in the expression of cardiac related genes
    5.4 Discussion
    5.5 Conclusion
Chapter Ⅵ Summary and Conclusion
References
Appendices
Acknowledgement
Published articles
    Scholarship Awards



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