隨著世界人口老齡化的加速,老年性骨質(zhì)疏松癥的發(fā)病率呈現(xiàn)出明顯的上升趨勢(shì).衰老相關(guān)的骨質(zhì)流失的機(jī)制研究對(duì)尋找有效的治療骨質(zhì)疏松藥物具有重要意義.天然產(chǎn)物為治療骨質(zhì)疏松癥藥物的發(fā)現(xiàn)提供了豐富的來(lái)源.川陳皮素是柑橘屬植物果實(shí)中含量最豐富的黃酮類成分之一,具有多種生物活性.在本研究中以20個(gè)月自然衰老小鼠為模型,連續(xù)15天腹腔注射給予川陳皮素,Micro-CT結(jié)果表明川陳皮素可以增加自然衰老小鼠骨體積分?jǐn)?shù)和降低骨小梁分離度,改善自然衰老小鼠的骨微觀結(jié)構(gòu),HE染色結(jié)果表明川陳皮素可增加骨小梁表面成骨細(xì)胞數(shù)量,川陳皮素處理小鼠血清中骨鈣蛋白（Osteocalcin）水平顯著增加.在細(xì)胞水平,川陳皮素處理小鼠前成骨細(xì)胞（MC3T3-E1）,Mki67水平顯著增加,骨堿性磷酸酶水平和骨鈣蛋白水平增加,茜素紅染色明顯增強(qiáng),表明川陳皮素能促進(jìn)MC3T3-E1細(xì)胞增殖、分化和礦化.進(jìn)一步機(jī)制研究發(fā)現(xiàn)川陳皮素可直接激活視黃酸受體相關(guān)孤兒受體α（retinoic acid receptor-related orphan receptors,RORα）,并以RORα依賴的方式下調(diào)骨硬化蛋白（Sclerostin,... 

【文章來(lái)源】：生物化學(xué)與生物物理進(jìn)展. 2020,47(08)北大核心SCICSCD

【文章頁(yè)數(shù)】：9 頁(yè)

【文章目錄】：
1 Materials and methods
    1.1 Animals
    1.2 Cell culture
    1.3 HE staining
    1.4 Alizarin red S staining
    1.5 ALP(alkaline phosphatase)activity assay
    1.6 Evaluation of serum markers of bone formation
    1.7 Analysis of bone by micro-computed tomography(Micro-CT)
    1.8 Western blot analysis
    1.9 Quantitative real-time PCR assay
    1.1 0 Data analysis
2 Results
    2.1 Nobiletin improves bone microstructure and bone formation in natural aging mice
    2.2 Nobiletin promotes osteoblast proliferation,differentiation and mineralization
    2.3 Nobiletin regulates RORαand SOST in natural aging mouse
    2.4 The effect of nobiletin on bone formation depends on RORα
3 Discussion


【參考文獻(xiàn)】：
期刊論文
[1]川陳皮素改善衰老認(rèn)知功能損傷的作用與機(jī)制（英文）[J]. 姚勤,張玉英,吳凱源,褚博煜,陳暢.  生物化學(xué)與生物物理進(jìn)展. 2019(06)
[2]D-ribose increases triglyceride via upregulation of DGAT in the liver[J]. Yao Chen,Lexiang Yu,Yan Wei,Yang Long,Yong Xu,Tao He,Rongqiao He.  Science China（Life Sciences）. 2019(06)
[3]Paracrine and endocrine actions of bone——the functions of secretory proteins from osteoblasts, osteocytes, and osteoclasts[J]. Yujiao Han,Xiuling You,Wenhui Xing,Zhong Zhang,Weiguo Zou.  Bone Research. 2018(02)



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