本文選題：低氧　切入點(diǎn)：大強(qiáng)度運(yùn)動(dòng)　出處：《中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的:探討低氧訓(xùn)練對(duì)大鼠小腸黏膜屏障功能的作用和機(jī)制。方法:5周齡雄性SD大鼠80只,隨機(jī)分為常氧對(duì)照組(C組,n=20)、常氧訓(xùn)練組(E組,n=20)、低氧對(duì)照組(HC組,n=20)、低氧訓(xùn)練組(HE組,n=20),通過(guò)人工低氧和游泳訓(xùn)練模擬高原訓(xùn)練,分別觀察2周、6周后大鼠小腸黏膜的組織結(jié)構(gòu)和超微結(jié)構(gòu),檢測(cè)血漿中二胺氧化酶(DAO)、D-乳酸(D-La)以及腸組織中腫瘤壞死因子(TNF-α)、核因子-κB(NF-κB)的含量和小腸黏膜中緊密連接蛋白o(hù)ccludin mRNA的表達(dá)量。結(jié)果:(1)實(shí)驗(yàn)6周后,透射電鏡觀察顯示,與常氧對(duì)照組相比,常氧訓(xùn)練組大鼠小腸微絨毛較稀疏,且排列不整齊,黏膜上皮細(xì)胞之間的間隙變寬,線粒體數(shù)量明顯減少,嵴模糊;低氧對(duì)照組小腸微絨毛長(zhǎng)度顯著縮短,排列較為紊亂,黏膜上皮細(xì)胞之間的間隙變寬,少量線粒體變性;低氧訓(xùn)練組小腸微絨毛數(shù)量明顯減少,長(zhǎng)度明顯縮短,腸絨毛出現(xiàn)倒伏,黏膜上皮細(xì)胞之間的間隙變寬,細(xì)胞結(jié)構(gòu)不清晰,線粒體變性,部分線粒體嵴模糊、甚至消失。(2)2周后,運(yùn)動(dòng)訓(xùn)練可顯著減少小腸絨毛數(shù)量(P0.01),顯著提高血漿DAO、D-La(P0.05)以及小腸組織中NF-κB含量(P0.01);而低氧暴露可顯著降低小腸組織occludin mRNA表達(dá)(P0.01),顯著提高血漿DAO、D-La(P0.05)以及小腸組織TNF-α(P0.05)、NF-κB(P0.01)含量;低氧暴露聯(lián)合運(yùn)動(dòng)訓(xùn)練對(duì)進(jìn)一步減少小腸絨毛數(shù)量、長(zhǎng)度和occludin mRNA表達(dá),升高血漿DAO、D-La以及小腸組織TNF-α、NF-κB含量均無(wú)顯著的交互作用(P0.05)。(3)6周后,運(yùn)動(dòng)訓(xùn)練及低氧暴露均顯著降低小腸絨毛數(shù)量(P0.01),增加血漿DAO、D-La含量(P0.01)以及小腸組織中TNF-α(P0.01,P0.05)和NF-κB含量(P0.01),顯著降低小腸組織中occludin mRNA的表達(dá)(P0.05);且低氧聯(lián)合運(yùn)動(dòng)訓(xùn)練對(duì)進(jìn)一步減少小腸絨毛數(shù)量、長(zhǎng)度(P0.01,P0.05),增加血漿中DAO、D-La含量(P0.01)具有顯著的交互作用。結(jié)論:(1)大強(qiáng)度運(yùn)動(dòng)訓(xùn)練以及低氧暴露均能導(dǎo)致腸道黏膜屏障功能受損,其損害程度與訓(xùn)練及低氧暴露時(shí)間有關(guān)。(2)低氧暴露以及大強(qiáng)度訓(xùn)練可能是通過(guò)增加小腸內(nèi)TNF-α、NF-κB的含量從而降低緊密連接蛋白o(hù)ccludin的表達(dá),最終導(dǎo)致小腸通透性增加,腸道黏膜屏障功能受損。
[Abstract]:Objective: to investigate the effect and mechanism of hypoxic training on intestinal mucosal barrier function in rats. They were randomly divided into normal oxygen control group (C), normoxic training group (E), hypoxia control group (HC) and hypoxia training group (HE). Altitude training was simulated by artificial hypoxia and swimming training. The tissue structure and ultrastructure of intestinal mucosa were observed in rats after 2 weeks and 6 weeks, respectively. The contents of TNF- 偽 and NF- 魏-魏 NF- 魏 B in plasma and the expression of tight junction protein occludin mRNA in intestinal mucosa were measured. Compared with the normoxic control group, the intestinal microvilli in the normoxic training group were sparse and irregular, the gap between the mucosal epithelial cells widened, the number of mitochondria decreased significantly and the ridge was blurred, the length of the intestinal microvilli in the hypoxic training group was significantly shorter than that in the hypoxic control group. In the hypoxic training group, the number and length of intestinal microvilli decreased significantly, the intestinal villi collapsed, and the gap between mucosal epithelial cells became wider. The cell structure was not clear, mitochondria degenerated, some mitochondria crest were blurred, and even disappeared 2 weeks later, Exercise training significantly decreased the number of villi and increased the content of NF- 魏 B in plasma DAO- D-La-P0.05) and small intestine tissues, while hypoxia exposure significantly decreased the expression of occludin mRNA in small intestine, significantly increased the content of plasma DAOD-LaP0.05) and the content of TNF- 偽 -P0.05- 魏 BP0.01 in small intestine. There was no significant interaction between hypoxia exposure and exercise training for further reducing the number of villi, length and occludin mRNA expression, increasing plasma DAOD-La and the content of TNF- 偽 -NF- 魏 B in small intestine. After 6 weeks, there was no significant interaction between hypoxia exposure and exercise training. Exercise training and hypoxic exposure significantly decreased the number of villi of small intestine and increased the content of DAOD-La in plasma and the contents of TNF- 偽 -P0.01P0.05) and NF- 魏 B in small intestine tissues, which significantly decreased the expression of occludin mRNA in intestinal tissues (P0.05). Reduce the number of villi, The increase of DAOOD-La content in plasma (P0.01) has a significant interaction. Conclusion: high intensity exercise training and hypoxia exposure can all result in impaired intestinal mucosal barrier function. The degree of injury is related to training and hypoxic exposure time.) hypoxia exposure and high intensity training may decrease the expression of tight junction protein occludin by increasing the content of TNF- 偽 -NF- 魏 B in the small intestine, resulting in the increase of intestinal permeability. Intestinal mucosal barrier function is impaired.
【作者單位】： 湖南師范大學(xué)體育學(xué)院;揚(yáng)州大學(xué)體育學(xué)院;
【基金】：揚(yáng)州市科技局2012科技攻關(guān)—社會(huì)發(fā)展科技攻關(guān)項(xiàng)目(2012112) 2016年國(guó)家重點(diǎn)研發(fā)計(jì)劃課題(2016yfd0400603-02)
【分類號(hào)】：R87

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 路瑛麗;趙鵬;馮連世;徐建方;朱珂;宗丕芳;;不同低氧暴露對(duì)大鼠有氧代謝潛能的影響[J];中國(guó)應(yīng)用生理學(xué)雜志;2010年03期

2 翁錫全;林潔如;林文韜;黃麗英;王仁綱;吳桂賢;;低氧暴露對(duì)運(yùn)動(dòng)性貧血大鼠抗氧化能力的影響[J];中國(guó)組織工程研究與臨床康復(fù);2007年17期

3 翁錫全;余群;林文_";徐國(guó)琴;黃利軍;李良鳴;王玉昕;;低氧暴露對(duì)運(yùn)動(dòng)誘導(dǎo)的血紅蛋白低下大鼠紅細(xì)胞參數(shù)和某些造血因子的影響[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2006年05期

4 林文_";邱烈峰;翁錫全;黃麗英;徐國(guó)琴;;低氧暴露對(duì)運(yùn)動(dòng)性低血紅蛋白大鼠血清鐵代謝的影響[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2007年01期

5 田云梅;聶鴻靖;劉嘉瀛;張延坤;張東祥;汪海;;低氧暴露對(duì)大鼠外周血T淋巴細(xì)胞活化的影響[J];中國(guó)應(yīng)用生理學(xué)雜志;2011年02期

6 賀道遠(yuǎn);曾凡星;倪吉祥;;間歇低氧對(duì)大鼠骨骼肌IGF-1和myostatin基因表達(dá)的影響[J];中國(guó)應(yīng)用生理學(xué)雜志;2008年04期

7 付中偉;徐玫;杜可軍;戴鵬;陳迎雷;黃冠鵬;蔡同建;駱文靜;陳景元;;低壓低氧暴露對(duì)大鼠空間記憶及海馬谷氨酸受體表達(dá)的影響[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2012年15期

8 劉曉東;周波;周其全;;銀杏葉制劑對(duì)模擬高原低氧暴露下腦微血管滲漏的干預(yù)作用[J];中國(guó)中醫(yī)急癥;2010年07期

9 吳雁,杜繼曾;低氧暴露對(duì)大鼠下丘腦CRF分泌的影響[J];中國(guó)應(yīng)用生理學(xué)雜志;2001年04期

10 李海洲;劉玉倩;王海濤;劉樹(shù)欣;趙斌;楊杰;;低氧暴露對(duì)大鼠骨骼肌L6細(xì)胞鐵代謝的影響[J];生理學(xué)報(bào);2011年04期

相關(guān)會(huì)議論文 前5條

1 葉鳴;李俊平;倪震;;低氧暴露對(duì)大鼠骨骼肌蛋白質(zhì)分解的作用[A];中國(guó)生理學(xué)會(huì)第23屆全國(guó)會(huì)員代表大會(huì)暨生理學(xué)學(xué)術(shù)大會(huì)論文摘要文集[C];2010年

2 羅涵;郭平;周其全;;TLR4/NF-κB在低氧暴露下大鼠腸道粘膜屏障功能損傷及細(xì)菌移位中的作用研究[A];第八屆全國(guó)生物醫(yī)學(xué)體視學(xué)學(xué)術(shù)會(huì)議、第十一屆全軍軍事病理學(xué)學(xué)術(shù)會(huì)議、第七屆全軍定量病理學(xué)學(xué)術(shù)會(huì)議論文（摘要）匯編[C];2012年

3 河春姬;胡揚(yáng);田野;包大鵬;雷雨晨;衣龍彥;;急性運(yùn)動(dòng)后低氧暴露對(duì)體內(nèi)自由基代謝的影響[A];第七屆全國(guó)體育科學(xué)大會(huì)論文摘要匯編（二）[C];2004年

4 王景玲;胡揚(yáng);曾凡星;;HiHiLo實(shí)施過(guò)程中SpO_2變化及與訓(xùn)練效果的關(guān)聯(lián)性研究[A];第八屆全國(guó)體育科學(xué)大會(huì)論文摘要匯編（一）[C];2007年

5 羅涵;郭平;周其全;;TLR4/NF-κB在低氧暴露下大鼠腸道粘膜屏障功能損傷及細(xì)菌移位中的作用研究[A];第十三屆中國(guó)體視學(xué)與圖像分析學(xué)術(shù)會(huì)議論文集[C];2013年

相關(guān)博士學(xué)位論文 前3條

1 張亞軍;低氧暴露對(duì)慢性疲勞綜合征小鼠認(rèn)知情緒的調(diào)節(jié)作用及機(jī)制研究[D];上海體育學(xué)院;2013年

2 黃巧婷;低氧暴露對(duì)大鼠急性離心運(yùn)動(dòng)后Dystrophin表達(dá)的影響[D];北京體育大學(xué);2016年

3 王基野;低溫低氧對(duì)學(xué)習(xí)記憶的影響及藥物防護(hù)研究[D];第四軍醫(yī)大學(xué);2013年

相關(guān)碩士學(xué)位論文 前4條

1 馬子龍;模擬低壓低氧暴露對(duì)SD大鼠生殖機(jī)能的損傷及機(jī)制的研究[D];石河子大學(xué);2016年

2 王娟;低氧暴露對(duì)大鼠攝食行為的影響[D];杭州師范大學(xué);2013年

3 陳媛;膽堿干預(yù)對(duì)低氧暴露小鼠學(xué)習(xí)記憶的改善及其tau蛋白機(jī)制研究[D];中國(guó)人民解放軍軍事醫(yī)學(xué)科學(xué)院;2014年

4 田云梅;高原低氧免疫損傷及其干預(yù)措施的研究[D];中國(guó)人民解放軍軍事醫(yī)學(xué)科學(xué)院;2009年

，

本文編號(hào)：1557973