【摘要】：目的研究VITEK MS對細(xì)菌性食物中毒樣品的快速檢測方法和效果,以提高致病菌檢測效率。同時即時對檢出的菌株進行同源性分析,以協(xié)助調(diào)查患者發(fā)生食物中毒的原因,預(yù)防食物中毒的再次發(fā)生。方法針對疑似細(xì)菌性食物中毒的6份樣品,增菌分離培養(yǎng)后使用VITEK MS進行檢測,并使用VITEK MS附屬的SARAMIS系統(tǒng)對檢出的菌株進行同源性分析。結(jié)果 VITEK MS共檢出3株條件致病菌糞腸球菌(Enterococcus faecalis)。同時SARAMIS系統(tǒng)通過質(zhì)譜圖進行同源性分析,表明3株菌具有高度同源性(90%以上)。結(jié)論 VITEK MS用于細(xì)菌性食物中毒的檢測簡單快速,對單個純菌落進行菌種鑒定具有很高的準(zhǔn)確性。同時還可以進行同源性分析,及時為現(xiàn)場流行病學(xué)調(diào)查提供幫助,具有良好的應(yīng)用價值。
[Abstract]:Objective to study the method and effect of rapid detection of bacterial food poisoning samples by VITEK MS in order to improve the detection efficiency of pathogenic bacteria. At the same time, the homology analysis of the strains was carried out to help investigate the causes of food poisoning and prevent the recurrence of food poisoning. Methods six samples of suspected bacterial food poisoning were isolated and cultured by VITEK MS, and the homology of the detected strains was analyzed by SARAMIS system attached to VITEK MS. Results A total of 3 conditional pathogenic bacteria, Enterococcus faecalis, (Enterococcus faecalis). Were detected by VITEK MS. At the same time, the homology analysis of SARAMIS system showed that the three strains had high homology (more than 90%). Conclusion VITEK MS is a simple and rapid method for the detection of bacterial food poisoning. At the same time, homology analysis can be carried out in time to provide help for field epidemiological investigation, which has good application value.
【作者單位】： 常州市疾病預(yù)防控制中心;
【基金】：常州市自然科學(xué)基金資助項目(項目編號:CE20165042)
【分類號】：R155.5
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本文編號：2465163