【摘要】：目的探討亞砷酸鈉對(duì)人上皮角質(zhì)形成細(xì)胞系(Hacat細(xì)胞)PI3K/AKT信號(hào)通路中細(xì)胞周期相關(guān)分子GSK-3β、CyclinD1的影響。方法將人Hacat細(xì)胞暴露于濃度分別為0、10、25、50、100μmol/L的亞砷酸鈉6 h,采用Western Blot法檢測(cè)細(xì)胞總蛋白的GSK-3β,磷酸化GSK-3β(p-GSK-3β),CyclinD1和磷酸化CyclinD1(p-CyclinD1)的蛋白表達(dá)水平。結(jié)果與對(duì)照組比較,10、25、50、100μmol/L染砷組總蛋白中p-GSK-3β和CyclinD1蛋白表達(dá)水平均顯著增高(P0.05),而p-CyclinD1蛋白表達(dá)水平顯著降低(P0.05)。結(jié)論亞砷酸鈉可影響Hacat細(xì)胞中的GSK-3β分子,使其磷酸化失活,進(jìn)而失去對(duì)細(xì)胞周期相關(guān)蛋白CyclinD1的磷酸化作用,從而促進(jìn)CyclinD1的表達(dá)。
[Abstract]:Aim to investigate the effect of sodium arsenite on GSK-3 尾 and CyclinD1 in PI3K/AKT signaling pathway of human epithelial keratinocytes (Hacat cells). Methods Human Hacat cells were exposed to sodium arsenite at concentrations of 0, 10, 25 and 50 100 渭 mol / L for 6 h, respectively. The expression levels of GSK-3 尾, phosphorylated GSK-3 尾 (p-GSK-3 尾), CyclinD1 and phosphorylated CyclinD1 (p-CyclinD1) were detected by Western Blot assay. Results compared with the control group, the expression levels of p-GSK-3 尾 and CyclinD1 protein in the total protein of 10,25,50100 渭 mol / L arsenic exposed group were significantly increased (P0.05), while the expression level of p-CyclinD1 protein was significantly decreased (P0.05). Conclusion Sodium arsenite can affect the GSK-3 尾 molecule in Hacat cells, make it phosphorylated and inactivated, and then lose the phosphorylation of CyclinD1, thus promote the expression of CyclinD1.
【作者單位】： 中國醫(yī)科大學(xué)公共衛(wèi)生學(xué)院勞動(dòng)衛(wèi)生教研室 "砷生物學(xué)作用評(píng)價(jià)及砷中毒防治"遼寧省重點(diǎn)實(shí)驗(yàn)室;
【基金】：遼寧省教育廳課題(編號(hào)L2010705)
【分類號(hào)】：R114

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黃秀蘭;崔國輝;周克元;;PI3K-Akt信號(hào)通路與腫瘤細(xì)胞凋亡關(guān)系的研究進(jìn)展[J];癌癥;2008年03期

2 孫嫣;田華;肖法Z，

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