【摘要】：應(yīng)用MTT比色法檢測(cè)不同濃度的亞油酸和α-亞麻酸對(duì)脂肪干細(xì)胞活力的影響,利用油紅O染色法和甘油三酯質(zhì)量摩爾濃度測(cè)定法檢測(cè)亞油酸和α-亞麻酸對(duì)脂肪干細(xì)胞成脂分化的影響。MTT結(jié)果表明,在一定濃度范圍內(nèi),α-亞麻酸能夠顯著增強(qiáng)細(xì)胞活力,但在較高濃度(60μmol/L)體現(xiàn)出明顯的細(xì)胞毒性作用;而亞油酸能夠顯著抑制脂肪干細(xì)胞活力。油紅O染色和甘油三酯測(cè)定結(jié)果表明,與對(duì)照組相比,亞油酸和α-亞麻酸均能促進(jìn)脂肪干細(xì)胞成脂分化;與α-亞麻酸相比,亞油酸對(duì)脂肪干細(xì)胞成脂分化的促進(jìn)作用更強(qiáng),且呈現(xiàn)劑量依賴性。
[Abstract]:The effects of different concentrations of linoleic acid and 偽 -linolenic acid on the activity of adipose stem cells were detected by MTT colorimetry. The effects of linoleic acid and 偽 -linolenic acid on adipogenic differentiation of adipose stem cells were detected by oil red O staining and triglyceride mass concentration determination. MTT results showed that, in a certain concentration range, the effect of linoleic acid and 偽 -linolenic acid on adipogenic differentiation of adipose stem cells was determined. 偽 -linolenic acid could significantly enhance cell viability, but showed obvious cytotoxicity at higher concentration (60 渭 mol/L). Linoleic acid significantly inhibited the activity of adipose stem cells. The results of oil red O staining and triglyceride determination showed that linoleic acid and 偽 -linolenic acid could promote adipogenic differentiation of adipose stem cells compared with control group. Compared with 偽-linolenic acid, linoleic acid promoted adipogenic differentiation of adipose stem cells in a dose-dependent manner.
【作者單位】： 大連工業(yè)大學(xué)食品學(xué)院;大連工業(yè)大學(xué)生物工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31371764,31370554) 遼寧省自然科學(xué)基金項(xiàng)目(2014026016)
【分類號(hào)】：R151
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本文編號(hào)：2382048