發(fā)布時間：2019-07-24 10:18

【摘要】：為了對菠蘿蛋白酶的抗過敏效果進行研究,采用透明質酸酶體外抑制試驗考察菠蘿蛋白酶在水解狀態(tài)和未水解狀態(tài)下對透明質酸酶的抑制效果,并運用小鼠被動過敏模型試驗評價菠蘿蛋白酶的抗過敏效果。結果表明,菠蘿蛋白酶質量濃度為0.6~3.6 mg/m L時,對透明質酸酶的抑制效果呈正相關;繼續(xù)增大菠蘿蛋白酶質量濃度,對透明質酸酶的抑制效果反而下降。菠蘿蛋白酶經(jīng)水解后發(fā)現(xiàn),當菠蘿蛋白酶的水解度為12.3%時,對透明質酸酶的抑制率達到最大為53.5%,對此水解產(chǎn)物進行超濾,發(fā)現(xiàn)其抑制效果主要由分子量在8~10 k Da的組分貢獻,此組分對透明質酸酶的抑制率達到最大59.6%。經(jīng)藍斑抑制試驗表明,相比中、高劑量組,低劑量組的菠蘿蛋白酶對小鼠皮膚藍斑的抑制效果最好,抑制率為55.87%,是陽性藥物息斯敏的1.06倍。菠蘿蛋白酶具有良好的抗過敏效果,為其進一步開發(fā)成為抗過敏劑應用于臨床提供理論基礎。
[Abstract]:In order to study the anti-allergic effect of the bromelain, the inhibition effect of the bromelain on the hyaluronidase in the hydrolysis and unhydrolyzed conditions was investigated by the in vitro inhibition test of the hyaluronidase. And the anti-allergic effect of the bromelain is evaluated by using a mouse passive allergy model test. The results showed that when the mass concentration of bromelain was 0.6-3.6 mg/ m L, the inhibitory effect on the hyaluronidase was positively correlated, and the quality of the bromelain was increased, and the inhibitory effect on the hyaluronidase was decreased. After the bromelain is hydrolyzed, when the degree of hydrolysis of the bromelain is 12.3%, the inhibition rate of the hyaluronidase is up to 53.5%, and the hydrolysis product is subjected to ultrafiltration, and the inhibition effect is mainly caused by the component with the molecular weight of 8-10k Da, The inhibition rate of the component to the hyaluronidase reached a maximum of 59.6%. The results of the blue-spot inhibition test show that the inhibitory effect of bromelain on the blue-spot of the mouse skin is the best, the inhibition rate is 55.87%, which is 1.06-fold higher than that of the positive drug. The bromelain has a good antiallergic effect and is further developed into an antiallergic agent for clinical application.
【作者單位】： 廣東海洋大學食品科技學院;
【基金】：廣東省高校重大科研項目培育計劃(GDOU2013050214) 廣東省自然科學基金博士啟動項目(2016A030310332)
【分類號】：R915

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