【摘要】：目的:建立同時測定復方苯海拉明滴鼻液中鹽酸麻黃堿、呋喃西林、鹽酸苯海拉明含量的方法。方法:采用反向高效液相色譜法。色譜柱為Inertsil ODS-3 C_18,流動相為0.05 mol/L磷酸二氫鉀緩沖液(p H 7.0)-乙腈(梯度洗脫),流速為1.0 m L/min,檢測波長為256 nm,柱溫35℃,進樣量為20μL。結果:鹽酸麻黃堿、呋喃西林、鹽酸苯海拉明檢測質量濃度線性范圍分別為122.1~366.3μg/m L(r=0.999 9)、5.2~15.5μg/m L(r=0.999 8)、31.5~94.5μg/m L(r=0.999 4);定量限分別為2.442、0.010、2.520μg/m L,檢測限分別為0.810、0.003、0.830μg/m L;精密度、穩(wěn)定性、重復性試驗的RSD1.0%;加樣回收率分別為99.2%~101.7%(RSD=0.9%,n=9),96.4%~102.0%(RSD=1.7%,n=9),100.2%~101.9%(RSD=0.4%,n=9)。結論:該方法操作簡便、結果準確、重復性好,適用于復方苯海拉明滴鼻液中鹽酸麻黃堿、呋喃西林、鹽酸苯海拉明含量的同時測定。
[Abstract]:Aim: to establish a method for simultaneous determination of ephedrine hydrochloride, furacillin and diphenhydramine hydrochloride in compound diphenhydramine nasal drops. Methods: reverse high performance liquid chromatography (RP-HPLC) was used. The mobile phase consisted of 0.05 mol/L potassium dihydrogen phosphate buffer (p H 7.0)-acetonitrile (gradient elution), the flow rate was 1.0ml / min, the detection wavelength was 256nm, column temperature at 35 鈩，

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