發(fā)布時間：2019-03-06 14:40

【摘要】：目的評價P-糖蛋白及有機陽離子轉(zhuǎn)運體對左氧氟沙星跨胃黏膜轉(zhuǎn)運的影響。方法用大鼠胃黏膜與Ussing chamber技術建立胃黏膜體外模型,分為空白組及加入P-糖蛋白抑制劑(環(huán)孢素A)或有機陽離子轉(zhuǎn)運體抑制劑(西咪替丁)的抑制劑組并進行雙向轉(zhuǎn)運實驗,用高效液相色譜法對左氧氟沙星進行定量分析,計算其表觀滲透系數(shù)(Papp)。結果加入環(huán)孢素A后,50μg·m L-1左氧氟沙星Papp(S-M)/Papp(M-S)由2.20變?yōu)?.06(P0.05);加入西咪替丁后,50μg·m L-1左氧氟沙星Papp(S-M)/Papp(M-S)與空白組比較,差異無統(tǒng)計學意義。結論 P-糖蛋白參與左氧氟沙星跨胃黏膜轉(zhuǎn)運過程,而有機陽離子轉(zhuǎn)運體不參與左氧氟沙星跨胃黏膜轉(zhuǎn)運過程。
[Abstract]:Objective to evaluate the effects of P-glycoprotein and organic cation transporter on transgastric transport of levofloxacin. Methods the rat gastric mucosa model in vitro was established by Ussing chamber technique. They were divided into two groups: blank group, inhibitor of P-glycoprotein (cyclosporin A) or cimetidine (cimetidine), and the two-way transport experiment was carried out, and the quantitative analysis of levofloxacin was carried out by high performance liquid chromatography (HPLC). Calculation of apparent permeability coefficient (Papp). Results after addition of cyclosporine A, 50 渭 g 路mL ~ (- 1) of levofloxacin Papp / Papp (Mus) was changed from 2.20 to 1.06 (P0.05). When cimetidine was added, there was no significant difference between 50 渭 g 路mL-1 levofloxacin Papp / Papp (Mus) group and the blank group. Conclusion P-glycoprotein is involved in the transgastric transport of levofloxacin, but organic cation transporters are not involved in the transgastric transport of levofloxacin.
【作者單位】： 安徽醫(yī)科大學第一附屬醫(yī)院科研處;安徽省消化系病重點實驗室;安徽醫(yī)科大學第一附屬醫(yī)院消化內(nèi)科;
【基金】：國家自然科學基金青年基金資助項目(81100269)
【分類號】：R965

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