【摘要】：目的建立LC-MS/MS法檢測小鼠血漿內(nèi)FG-4592的濃度,研究FG-4592在小鼠體內(nèi)的藥代動力學。方法KM小鼠腹腔注射FG-4592 5 mg/kg,不同時間點采集血漿,樣品經(jīng)甲醇沉淀。選用Agilent Zorbax SB-Aq-C18色譜柱(2.1 mm×100 mm,3.5μm),柱溫25℃,流動相為甲醇-水(含5 mmol/L甲酸銨,0.1%甲酸)(90∶10),流速0.35 m L/min。采用ESI離子源,正離子模式多反應(yīng)監(jiān)測,離子通道分別為m/z 353.1→m/z 278.1(FG-4592)和m/z294.9→m/z 235.0(內(nèi)標物)。結(jié)果測定血漿中FG-4592的線性范圍為1~1000 ng/m L,日內(nèi)及日間精密度均10%,準確度在90%~105%范圍內(nèi),方法學考察均符合生物樣品的分析要求。藥代動力學參數(shù):Cmax為(22.80±0.36)μg/m L,AUC(0-72)為(31.64±0.97)(μg·h)/m L,t1/2為8.81 h。結(jié)論本研究建立的FG-4592血藥濃度測定方法可行,該藥物在血液中吸收良好,測定結(jié)果可為藥物的基礎(chǔ)研究提供依據(jù)。
[Abstract]:Objective to establish a LC-MS/MS method for the determination of FG-4592 in mouse plasma and to study the pharmacokinetics of FG-4592 in mice. Methods KM mice were injected intraperitoneally with FG-4592 5 mg/kg, to collect plasma at different time points, and the samples were precipitated by methanol. The Agilent Zorbax SB-Aq-C18 column (2.1 mm 脳 100 mm,3.5 渭 m),) was used at 25 鈩，

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