【摘要】：目的:建立同時測定復方螺內酯凝膠中螺內酯和紅霉素含量的方法。方法:采用高效液相色譜法。色譜柱為Thermo-Hypersil ODS2-C18,流動相為0.1 mol/L磷酸二氫銨溶液(用三乙醇胺調節(jié)p H為7.0)-乙腈(60∶40,V/V),流速為1.0 ml/min,檢測波長為215和238 nm,柱溫為30℃,進樣量為5μl。結果:螺內酯和紅霉素的檢測質量濃度分別在0.251 6~5.032 0、0.577 2~11.544 0μg/ml范圍內與其峰面積呈良好的線性關系(r=0.999 9、0.999 9);精密度、穩(wěn)定性、重復性試驗的RSD≤0.83%;平均回收率分別為97.8%(RSD=0.74%,n=9)、96.7%(RSD=2.60%,n=9)。結論:本方法操作簡便,重復性好,結果準確、可靠,可用于復方螺內酯凝膠的質量控制。
[Abstract]:Objective: to establish a method for simultaneous determination of spironolactone and erythromycin in compound spironolactone gel. Methods: high performance liquid chromatography was used. The column consisted of Thermo-Hypersil ODS2-C18, mobile phase, 0.1 mol/L ammonium dihydrogen phosphate solution (adjusted by triethanolamine to pH 7.0) -acetonitrile (60: 40 V / V), flow rate 1.0 ml/min, detection wavelength 215 and 238 nm, The column temperature is 30 鈩，

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