【摘要】：目的通過優(yōu)化CCK-8的實驗條件,提高檢測藥物影響腫瘤細胞增殖的敏感性和可靠性。方法以人肺癌A549細胞和白血病Molt-4細胞為研究對象,CCK-8法和MTT法分別檢測細胞增殖的變化,并與Coulter細胞計數(shù)法和克隆形成率法進行比較。結(jié)果在相同的細胞數(shù)下,CCK-8法的檢測靈敏度明顯高于MTT法。以細胞密度1 000個/孔接種,抗腫瘤藥物多柔比星處理96 h后,CCK-8法檢測到的變化與克隆形成率法相當(dāng)。分析Molt-4懸浮細胞發(fā)現(xiàn):CCK-8法與Coulter細胞計數(shù)法的檢測靈敏度無明顯差異(P0.05)。結(jié)論優(yōu)化實驗條件后,CCK-8法是一種靈敏度高、可靠性良好的細胞增殖檢測方法。
[Abstract]:Objective to improve the sensitivity and reliability of drug detection for tumor cell proliferation by optimizing the experimental conditions of CCK-8. Methods the proliferation of human lung cancer A549 cells and leukemia Molt-4 cells were detected by CCK-8 and MTT, and compared with Coulter cell count and clone formation rate. Results at the same number of cells, the sensitivity of CCK-8 assay was significantly higher than that of MTT method. The cell density was 1 000 / well and the antitumor drug doxorubicin was treated for 96 h. The changes detected by CCK-8 method were similar to those detected by Clone formation method. Analysis of Molt-4 suspension cells showed that there was no significant difference in sensitivity between CCK-8 method and Coulter cell count method (P0.05). Conclusion after optimizing the experimental conditions, CCK-8 method is a sensitive and reliable method for cell proliferation detection.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院醫(yī)藥生物技術(shù)研究所;
【基金】：國家自然科學(xué)基金課題(No.81273553) 北京協(xié)和醫(yī)學(xué)院2015年研究生創(chuàng)新基金(No.2015-1007-18)
【分類號】：R96

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