【摘要】：為了探討殼寡糖(COS)對He La細胞和A549細胞自噬性死亡的影響,利用不同濃度COS處理細胞后MTT法檢測細胞的增殖抑制情況,采用MDC染色法觀察自噬泡的形成,免疫熒光法檢測LC3在細胞內(nèi)的表達,Western-blot分析COS處理He La和A549細胞不同時間后細胞內(nèi)LC3-Ⅱ/LC3-Ⅰ變化。結果表明,5.0 mg·m L-1COS對兩種細胞的增殖抑制率最大,分別為52.15%和57.63%(P0.01)。與陰性對照相比,經(jīng)COS處理后的細胞MDC染色細胞內(nèi)熒光強度增加,且陽性信號聚集于核周區(qū)域;免疫熒光檢測發(fā)現(xiàn)LC3陽性顆粒數(shù)明顯增加,表明細胞內(nèi)自噬體的數(shù)量增多。Western-blot檢測分析隨著COS處理細胞時間延長,LC3-Ⅱ/LC3-Ⅰ比值逐漸增大,顯示細胞內(nèi)自噬活性增強。由此推測COS能夠通過誘導He La和A549細胞的自噬性死亡來抑制細胞增殖,為COS的抗腫瘤作用機制的研究提供理論依據(jù)。
[Abstract]:In order to investigate the effect of chitosan oligosaccharide (COS) on autophagy death of He La cells and A549 cells, the inhibition of cell proliferation was detected by MTT method after treated with different concentrations of COS, and the formation of autophagy was observed by MDC staining. The expression of LC3 in He La and A549 cells was detected by Western blot with immunofluorescence assay. The changes of LC3- 鈪，

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