本文選題：K細胞 + 自噬　； 參考：《中國實驗血液學雜志》2017年02期

【摘要】：目的:探討高三尖杉酯堿(HHT)單用或與自噬抑制劑3-甲基腺嘌呤(3-MA)聯(lián)用對K562細胞凋亡及自噬的影響。方法:K562細胞在HHT(10 ng/ml)作用下1-8 d,采用流式細胞術檢測細胞凋亡;采用RT-PCR法、Western blot法和透射電子顯微鏡檢測細胞自噬。結果:在HHT作用前期K562細胞凋亡率逐漸升高,而在后期則下降。HHT作用后,K562細胞的Beclin1表達及LC3Ⅱ/Ⅰ蛋白比值先下降后升高。HHT單用時,激活型caspase-3蛋白表達前期逐漸上升,而在后期下降。與自噬抑制劑3-MA聯(lián)用組的K562細胞的Beclin1表達和LC3Ⅱ/Ⅰ的比值持續(xù)下降,而激活型caspase-3蛋白表達則逐漸升高。結論:HHT可誘導K562細胞凋亡,但HHT持續(xù)作用可誘導K562細胞發(fā)生自噬,與3-MA聯(lián)用則增強HHT的細胞毒作用。
[Abstract]:Aim: to investigate the effects of homoharringtonine (HHT) alone or in combination with autophagy inhibitor 3-methyladenine 3-MAon on apoptosis and autophagy in K562 cells. Methods Cell apoptosis was detected by flow cytometry (FCM), and autophagy was detected by RT-PCR assay and transmission electron microscope (TEM). Results: the apoptotic rate of K562 cells increased gradually at the early stage of HHT treatment, but the Beclin1 expression and the ratio of LC3 鈪，

本文編號：1802294