本文選題：適配體 + siRNA��； 參考：《中國生物工程雜志》2015年01期

【摘要】：為探討適配體介導的脂質體靶向遞送siRNA的可行性,采用前列腺癌細胞膜表面抗原(PSMA)的適配體A10-3.2與脂質體結合,構建適配體-脂質體靶向遞送體系(Apt-LP),并利用Apt-LP遞送p EGFP-N1質粒和Bcl2 siRNA到前列腺癌細胞LNCa P(PSMA+)和PC-3(PSMA-),轉染48h后,用熒光顯微鏡檢測綠色熒光蛋白的表達,q PCR檢測Bcl2 mRNA表達,蛋白印記法檢測Bcl2蛋白表達,Hoechst 33258核染法分析體外抗腫瘤活性。結果顯示:與脂質體遞送體系相比,Apt-LP顯著提高遞送p EGFP-N1質粒和Bcl2 siRNA到靶細胞LNCa P(PSMA+)的效率;顯著提高Bcl2 siRNA誘導的靶細胞LNCa P(PSMA+)Bcl2基因沉默效應,更有效的誘導靶細胞LNCa P(PSMA+)凋亡。結果表明:Apt-LP是一種有效的siRNA靶向遞送體系,具有潛在的臨床應用價值。
[Abstract]:In order to investigate the feasibility of aptamer mediated liposome targeting delivery of siRNA, the aptamer A10-3.2 of prostate cancer cell membrane surface antigen (PSMA) was used to bind to liposome.Aptamer liposome targeting delivery system (Apt-LPN) was constructed, and Apt-LP was used to deliver p EGFP-N1 plasmid and Bcl2 siRNA to prostate cancer cell line LNCa P(PSMA) and PC-3PSMA-G. After transfection for 48 h, the expression of green fluorescent protein (GFP) and Bcl2 mRNA were detected by fluorescence microscope.The expression of Bcl2 protein was detected by protein imprinting method. Hoechst 33258 nuclear staining method was used to analyze the anti-tumor activity in vitro.The results showed that compared with liposome delivery system, Apt-LP significantly increased the efficiency of delivering p EGFP-N1 plasmids and Bcl2 siRNA to target LNCa P(PSMA, increased the silencing effect of LNCa P(PSMA Bcl2 gene induced by Bcl2 siRNA, and more effectively induced LNCa P(PSMA apoptosis.The results show that: Apt-LP is an effective siRNA targeting delivery system and has potential clinical application value.
【作者單位】： 蘭州交通大學化學與生物工程學院;甘肅省醫(yī)學科學研究院;
【基金】：甘肅省中青年基金(1107RJYA033) 蘭州交通大學校青年基金(2013012)資助項目
【分類號】：R943

【共引文獻】

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