本文選題：鹽酸去氫駱駝蓬堿 + 脂質體　； 參考：《中國藥學雜志》2015年08期

【摘要】：目的制備cRGD介導的鹽酸去氫駱駝蓬堿長循環(huán)脂質體,對其體外性質進行考察,建立cRGD介導的鹽酸去氫駱駝蓬堿長循環(huán)脂質體的最終制備方法。方法首先建立鹽酸去氫駱駝蓬堿的含量測定方法,采用逆相蒸發(fā)法制備脂質體,并采用主動載藥法和被動載藥法兩種方法包裹鹽酸去氫駱駝蓬堿。在此基礎上,利用cRGD-DSPE-2000取代部分磷脂成分,制備cRGD修飾的鹽酸去氫駱駝蓬堿長循環(huán)脂質體,并對不同方法制得的脂質體的粒徑、電位和包封率進行測定,從而最終確定cRGD修飾的鹽酸去氫駱駝蓬長循環(huán)脂質體的制備方法。在此基礎上,進行了脂質體的釋放度考察。結果除體積分數(shù)100%甲醇外,各標準曲線相關性均較好。被動載藥法、主動載藥法以及cRGD修飾的長循環(huán)脂質體的粒徑分別為227.2、246.3、241.9 nm,ξ電位均在20~30 m V左右,包封率分別為(36.78±6.82)%、(81.77±7.61)%、(80.02±1.27)%。cRGD修飾長循環(huán)脂質體與普通脂質體和原料相比,釋放更加緩慢平穩(wěn)。結論采用主動載藥法制備cRGD修飾的鹽酸去氫駱駝蓬堿脂質體具備一定的可行性。
[Abstract]:Objective to prepare cRGD mediated long circulating liposome of deshlorophorine hydrochloride and to study its properties in vitro and to establish the final preparation method of cRGD mediated long circulating liposome of dehydrolampine hydrochloride.Methods at first, a method was established for the determination of dehydrolampine hydrochloride. Liposomes were prepared by reverse phase evaporation, and the active drug loading method and passive drug loading method were used to encapsulate the dehydrolordine hydrochloride.On this basis, cRGD-DSPE-2000 was used to replace some phospholipid components to prepare long-circulating liposomes modified with cRGD. The particle size, potential and entrapment efficiency of the liposomes prepared by different methods were determined.Finally, the preparation method of long circulating liposomes modified by cRGD was determined.On this basis, the release of liposome was investigated.Results except 100% methanol, the correlation of standard curves was good.The particle size of long-cycle liposomes modified by passive drug loading, active drug loading and cRGD modification were 227.2246.3nm, 尉 potential were about 200.30mV, and the entrapment efficiency were 36.78 鹵6.82 鹵80.02 鹵80.02 鹵80.02 鹵1.27)%.cRGD, respectively, and the release was slower and more stable than that of ordinary liposomes and raw materials.Conclusion it is feasible to prepare cRGD modified liposome of dehydrolampine hydrochloride by active drug loading method.
【作者單位】： 新疆醫(yī)科大學;
【分類號】：R943
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本文編號：1750549