本文選題：骨肉瘤　切入點(diǎn)：索拉菲尼　出處：《中國臨床藥理學(xué)雜志》2015年20期

【摘要】：目的研究索拉菲尼對骨肉瘤細(xì)胞增殖和凋亡的作用及其分子機(jī)制。方法將人骨肉瘤細(xì)胞(HOS細(xì)胞)分為對照組及索拉菲尼組,用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽比色法觀察索拉菲尼對HOS細(xì)胞活力的影響,用蛋白質(zhì)印跡法檢測凋亡相關(guān)蛋白變化,用Real-time PCR檢測let-7d變化情況。結(jié)果索拉菲尼可抑制HOS細(xì)胞活力,并呈劑量和濃度依賴性;同時,索拉菲尼可以抑制Lin28表達(dá),同時促進(jìn)let-7d表達(dá);過表達(dá)Lin28可以逆轉(zhuǎn)索拉菲尼的促凋亡作用。結(jié)論索拉菲尼通過調(diào)控Lin28/le-7d環(huán)路來促進(jìn)骨肉瘤細(xì)胞凋亡。
[Abstract]:Objective to study the effect of Solafenil on proliferation and apoptosis of osteosarcoma cells and its molecular mechanism. Methods Human osteosarcoma cell line HOS cells were divided into control group and Solafenil group. The effects of Solafenil on the activity of HOS cells were observed by using 3-tiaphane 5-dimethylthiazole-2-triazolium 5-diphenyl tetrazolium bromide colorimetry, and the changes of apoptosis-related proteins were detected by Western blotting. Real-time PCR was used to detect the changes of let-7d. Results Solafinil inhibited the activity of HOS cells in a dose-and dose-dependent manner, and at the same time, it inhibited the expression of Lin28 and promoted the expression of let-7d. Overexpression of Lin28 can reverse the apoptosis-promoting effect of Solafini. Conclusion Solafini can promote the apoptosis of osteosarcoma cells by regulating the Lin28/le-7d loop.
【作者單位】： 浙江中醫(yī)藥大學(xué)附屬第一醫(yī)院西藥房;
【基金】：浙江省醫(yī)藥衛(wèi)生科技計劃基金資助項目(2014KYB315)
【分類號】：R96

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 洪黎明;楊曉葵;;let-7c對卵巢功能的調(diào)控作用[J];國際生殖健康/計劃生育雜志;2015年04期

2 饒麗華;黃孝文;許勝;;Lin28/Let-7調(diào)節(jié)環(huán)分子機(jī)制及相關(guān)因子研究進(jìn)展[J];臨床耳鼻咽喉頭頸外科雜志;2014年09期

3 張靜;徐小隔;龔哲;趙紹云;何霞;王天舒;焦淑潔;滕軍放;賈延R，

本文編號：1660656