本文選題：真菌　切入點(diǎn)：硒礦床區(qū)　出處：《北京大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年06期 　論文類(lèi)型：期刊論文

【摘要】：利用單菌落挑選與劃線(xiàn)培養(yǎng)技術(shù),從硒礦區(qū)土壤樣品中分離純化真菌。通過(guò)搖床發(fā)酵培養(yǎng),提取制備發(fā)酵樣品。采用MTT法,用MDA-MB-231細(xì)胞、Hela細(xì)胞、SMMC-7721細(xì)胞測(cè)試發(fā)酵樣品的抗腫瘤活性。從采自湖北恩施全球唯一獨(dú)立硒礦床區(qū)的6個(gè)不同土樣中分離真菌73株。其中,對(duì)人宮頸癌Hela細(xì)胞的IC_(50)小于90μg/m L的菌株32株,對(duì)人乳腺癌MDA-MB-231細(xì)胞的IC_(50)小于90μg/m L的菌株13株,對(duì)人肝癌SMMC-7721細(xì)胞的IC_(50)小于90μg/m L的菌株9株。這些活性菌株為篩選抗腫瘤天然產(chǎn)物提供了良好的藥源菌株。
[Abstract]:The fungi were isolated and purified from the soil samples of selenium mining area by single colony selection and underlined culture. The fermentation samples were extracted and prepared by shaking table fermentation. MTT method was used to isolate and purify the fungi from the soil samples. The antitumor activity of fermentative samples was tested by MDA-MB-231 cell line Hela cell line SMMC-7721. 73 strains of fungi were isolated from 6 different soil samples collected from the only independent selenium deposit area in Enshi, Hubei Province. For human cervical cancer Hela cells, 32 strains were less than 90 渭 g / mL, and 13 strains for human breast cancer MDA-MB-231 cells were less than 90 渭 g / mL. Nine strains of SMMC-7721 cells with less than 90 渭 g / mL were isolated from human hepatoma SMMC-7721 cells. These active strains provided a good drug source for screening natural anti-tumor products.
【作者單位】： 生物資源保護(hù)與利用湖北省重點(diǎn)實(shí)驗(yàn)室湖北民族學(xué)院武陵山天然藥物研究所;國(guó)家環(huán)境保護(hù)飲用水水源地管理技術(shù)重點(diǎn)實(shí)驗(yàn)室深圳市環(huán)境科學(xué)研究院;
【基金】：湖北省高校優(yōu)勢(shì)特色學(xué)科群建設(shè)計(jì)劃(鄂學(xué)位[2015]5) 生物資源保護(hù)與利用湖北省重點(diǎn)實(shí)驗(yàn)室重點(diǎn)項(xiàng)目(PKLHB1504) 深圳市科技研發(fā)資金(JCYJ20130402094730839) 湖北民族學(xué)院博士啟動(dòng)基金(4138004)和湖北民族學(xué)院大學(xué)生創(chuàng)新創(chuàng)業(yè)項(xiàng)目(2016CX0715)資助
【分類(lèi)號(hào)】：R915

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