本文關(guān)鍵詞： 福美司坦 同位素比質(zhì)譜 興奮劑　出處：《中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志》2015年06期 　論文類(lèi)型：期刊論文

【摘要】：目的:建立對(duì)福美司坦藥物來(lái)源的檢測(cè)方法,并進(jìn)行人體代謝的檢測(cè)研究。方法:采用酶解、提取、高效液相色譜純化等方法對(duì)尿樣進(jìn)行預(yù)處理,應(yīng)用同位素比質(zhì)譜儀測(cè)定相關(guān)組分的同位素比(13C/12C,δ值),建立檢測(cè)方法。本實(shí)驗(yàn)中招募2名男性健康受試者,每位受試者口服福美司坦后收集尿樣,應(yīng)用所建立的檢測(cè)方法對(duì)尿樣進(jìn)行分析,探討福美司坦在人體內(nèi)代謝過(guò)程中的檢測(cè)規(guī)律。結(jié)果:建立的分析方法精密度及重現(xiàn)性RSD均小于1%。受試者尿樣中福美斯坦的δ值在服藥后迅速降低,并在27小時(shí)內(nèi)保持在約為-31‰。內(nèi)源性雄性激素代謝物雄酮androsterone和本膽烷醇酮etiocholanolone的δ值無(wú)顯著性變化。結(jié)論:在興奮劑檢測(cè)中確定以福美司坦藥物原形為目標(biāo)化合物進(jìn)行來(lái)源分析,建立了檢測(cè)福美斯坦興奮劑的同位素比質(zhì)譜分析方法。
[Abstract]:Objective: to establish a method for the detection of the origin of Fumestan and to study the metabolism of human body. Methods: urine samples were pretreated by enzymolysis, extraction and purification by HPLC. The isotopic ratio of 13C / 12C, 未 value of the related components was determined by means of isotope mass spectrometer, and a method was established. Two healthy male subjects were recruited in this experiment, and each of them took Fumestan orally and collected urine samples. The urine samples were analyzed by the established method. Objective: to study the detection rule of Fumestan during metabolism in human body. Results: the precision and reproducibility of the method were less than 1. The 未 value of Fumestan in urine decreased rapidly after taking medicine. The 未 values of androsterone androsterone and etiocholanolone, the endogenous metabolites of androgen, did not change significantly within 27 hours. Conclusion: the target combination of Fumestan drug in stimulant detection is determined. For source analysis, A mass spectrometry method for the determination of fumestan stimulants was established.
【作者單位】： 國(guó)家體育總局反興奮劑中心;北京體育大學(xué);北京中醫(yī)藥大學(xué);
【基金】：國(guó)家體育總局重點(diǎn)研究領(lǐng)域攻關(guān)課題(編號(hào):2013B003)
【分類(lèi)號(hào)】：R927;O657.63

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 彭暉;抗腫瘤藥、芳香化酶抑制劑福美司坦[J];國(guó)外醫(yī)藥.合成藥.生化藥.制劑分冊(cè);1994年02期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 彭暉;抗腫瘤藥、芳香化酶抑制劑福美司坦[J];國(guó)外醫(yī)藥.合成藥.生化藥.制劑分冊(cè);1994年02期

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 胡永洲,劉滔,姚愛(ài)平,楊大為;福美司坦的合成方法改進(jìn)[J];浙江大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2001年01期

，

本文編號(hào)：1506108