本文關(guān)鍵詞： 大氣細顆粒物 代謝輪廓 主成分分析 毒性機理　出處：《分析化學》2017年08期 　論文類型：期刊論文

【摘要】：建立了基于氣相色譜-質(zhì)譜聯(lián)用技術(shù)(GC-MS)分析大氣細顆粒物(PM_(2.5))滴注對小鼠肺組織代謝輪廓的影響的研究方法。通過分析肺組織細胞內(nèi)代謝物的變化,研究不同濃度PM_(2.5)對小鼠肺組織代謝的毒性機制。鼻腔分別滴注0、7.5、20.0和37.5 g/L的PM_(2.5)懸液,提取肺組織胞內(nèi)物質(zhì),預(yù)處理后進行GC-MS分析,結(jié)合主成分分析法(PCA)、偏最小二乘判別分析法(PLS-DA)進行數(shù)據(jù)解析,通過PLS-DA得分圖可將不同PM_(2.5)染毒濃度下的肺組織胞內(nèi)物質(zhì)明顯區(qū)分。運用PLS-DA載荷圖及模型的變量重要性因子(VIP)值,發(fā)現(xiàn)了7種代謝物可作為區(qū)別不同濃度PM_(2.5)下代謝組的潛在生物標志物,分別為丙氨酸、纈氨酸、亮氨酸、鳥氨酸、延胡索酸、檸檬酸、嘌呤(p0.01)。代謝途徑分析結(jié)果表明,PM_(2.5)滴注使小鼠肺組織受到氧化損傷,氧化應(yīng)激反應(yīng)增強,抑制了三羧酸循環(huán)(TCA循環(huán))及嘌呤代謝。本研究為深入解析PM_(2.5)致毒機理提供了新的方法及理論依據(jù)。
[Abstract]:A gas chromatography-mass spectrometry (GC-MS) method for the analysis of fine atmospheric particulate matter was established. Methods the effect of drip on the metabolic profile of lung tissue in mice was studied. The changes of intracellular metabolites in lung tissue were analyzed. To study the toxic mechanism of different concentrations of PMS-2.5) on the metabolism of lung tissue in mice, the nasal cavity was infused with 0.7.5g / L and 37.5 g / L PMSP 2.5) suspension respectively. Extracellular substances were extracted from lung tissue and analyzed by GC-MS after pretreatment. The data were analyzed with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The intracellular matter of lung tissue could be distinguished obviously by PLS-DA score. The PLS-DA load map and the VIPs of the model were used. Seven metabolites were found to be potential biomarkers for differentiating different concentrations of PMS-2.5) in metabolic groups, namely alanine, valine, leucine, ornithine, fumaric acid and citric acid. The results of metabolic pathway analysis showed that the mice lung tissue was damaged by oxygen oxidation and the oxidative stress reaction was enhanced. TCA cycle and purine metabolism were inhibited. This study provides a new method and theoretical basis for the further analysis of the toxic mechanism of PMSP _ (2.5).
【作者單位】： 北京工商大學食品學院;北京市食品添加劑工程技術(shù)研究中心;
【基金】：國家自然科學基金項目(No.21407006)資助~~
【分類號】：O657.63;R994.6

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