本文關鍵詞： 耳蝸核前腹側核 叢細胞 發(fā)育 電生理　出處：《南方醫(yī)科大學學報》2014年07期 　論文類型：期刊論文

【摘要】：目的通過全細胞膜片鉗技術研究新生SD乳鼠耳蝸核前腹核(AVCN)中叢細胞在發(fā)育過程中的電生理特性變化。方法運用全細胞膜片鉗記錄出生后5~21 d的SD乳鼠耳蝸核前腹核中叢細胞的動作電位(AP)反應及微小興奮性突觸后電流(mEPSC),再通過相關數(shù)據(jù)采集及分析軟件分析動作電位半峰寬和mEPSC的10%~90%上升時間(10%~90%Risetime)及時間常數(shù),并對此做進一步統(tǒng)計分析處理,進而比較不同年齡段新生SD乳鼠的耳蝸核前腹核中叢細胞的電生理反應特性。結果在叢細胞動作電位半峰寬的統(tǒng)計分析中發(fā)現(xiàn),叢細胞動作電位的時間精確性隨著出生后年齡的增加而增加,在聽覺建立(出生后12 d)附近變化停止。叢細胞mEPSC的時間精確性也隨著出生后年齡的增加而增加,在聽覺建立附近變化停止。結論耳蝸核前腹核中叢細胞功能的修飾先于聽覺的建立。
[Abstract]:Objective to study the electrophysiological characteristics of plexus cells in the anterior ventral nucleus of the cochlear nucleus (AVCNN) of newborn SD rats by whole-cell patch clamp technique. Methods whole cell patch clamp was used to record the precochlear nucleus precochlear nucleus of neonatal SD rats at 5 to 21 days after birth. The action potential (APP) response and the minimal excitatory postsynaptic current (mEPSCN) of the plexus cells in the ventral nucleus were analyzed. The half peak width of the action potential and the 1090% rise time of mEPSC and the time constant were analyzed by the software of relevant data acquisition and analysis. The electrophysiological responses of the plexus cells in the anterior ventral nucleus of the cochlear nucleus of newborn SD rats of different ages were compared. Results in the statistical analysis of the half peak width of the action potential of the plexus cells, the electrophysiological response of the plexus cells in the anterior ventral nucleus of the cochlear nucleus was compared. The temporal accuracy of the action potential of plexus cells increased with the increase of postnatal age, and stopped in the vicinity of auditory establishment (12 days after birth). The temporal accuracy of mEPSC of plexus cells also increased with the increase of postnatal age. Conclusion the functional modification of the plexus cells in the anterior ventral nucleus of the cochlear nucleus is prior to the establishment of the auditory system.
【作者單位】： 南方醫(yī)科大學基礎醫(yī)學院生理學教研室;
【基金】：國家自然科學基金(31171059)~~
【分類號】：R764

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