【摘要】：目的 :研究胰島素抵抗(IR)及脂聯(lián)素(APN)缺乏對小鼠心肌重構(gòu)的影響。方法 :選擇16只野生C57小鼠隨機分為對照組和IR組,每組8只;16只APN基因敲除(APNKO)C57小鼠隨機分為APNKO組和APNKO+IR組,每組各8只。對照組和APNKO組給予普通飼料,IR組和APNKO+IR組給予高脂飼料誘導產(chǎn)生IR。喂養(yǎng)12周后取血測定總膽固醇、甘油三酯、空腹血糖和空腹胰島素水平,取心臟測量心臟重量和左心室重量。取左心室心肌做蘇木素伊紅(HE)染色和馬松(Masson)染色,觀察各組心肌結(jié)構(gòu)改變及纖維化的程度;用免疫組化法和蛋白免疫印跡法檢測心肌中基質(zhì)金屬蛋白酶-9(MMP-9)和APN表達量的差異。結(jié)果 :與對照組比較,IR組、APNKO組和APNKO+IR組中小鼠的總膽固醇、甘油三酯、空腹血糖和空腹胰島素、心臟重量和左心室重量均有增加(P0.05);HE染色及Masson染色結(jié)果顯示:與對照組比較,IR組、APNKO組和APNKO+IR組中小鼠的心肌肥大程度更高;免疫組化及蛋白免疫印跡結(jié)果顯示:與對照組相比,IR組、APNKO組和APNKO+IR組中小鼠心肌APN表達明顯減低,心肌MMP-9表達明顯增加(P0.05)。結(jié)論 :APN缺乏和IR導致心肌重構(gòu),且兩者起協(xié)同促進作用。
[Abstract]:Aim: to study the effects of insulin resistant (IR) and adiponectin (APN) deficiency on myocardial remodeling in mice. Methods: sixteen wild C57 mice were randomly divided into control group and IR group, and 16 APN gene knockout (APNKO) C57 mice were randomly divided into APNKO group and APNKO IR group with 8 mice in each group. The control group and APNKO group were given ordinary diet, and the IR group and APNKO IR group were given high fat diet to induce IR. production. After 12 weeks of feeding, total cholesterol, triglyceride, fasting blood glucose and fasting insulin were measured. Heart weight and left ventricular weight were measured. Left ventricular myocardium was stained with hematoxylin eosin (HE) and Ma Song (Masson) to observe the changes of myocardial structure and the degree of fibrosis. The expression of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-9 (APN) in myocardium were detected by immunohistochemistry and Western blot. Results: compared with the control group, the total cholesterol, triglyceride, fasting blood glucose, fasting insulin, heart weight and left ventricular weight were increased in IR, APNKO and APNKO IR groups (P0.05). The results of HE staining and Masson staining showed that the degree of myocardial hypertrophy in IR group, APNKO group and APNKO IR group was higher than that in control group. The results of immunohistochemistry and Western blot showed that compared with the control group, the expression of myocardial APN in IR group, APNKO group and APNKO IR group was significantly decreased, and the expression of myocardial MMP-9 was significantly increased (P0.05). Conclusion: APN deficiency and IR lead to myocardial remodeling, and they play a synergistic role in promoting myocardial remodeling.
【作者單位】： 寧夏醫(yī)科大學心血管內(nèi)科;寧夏醫(yī)科大學總醫(yī)院心臟中心干部病房;寧夏醫(yī)科大學基礎醫(yī)學院;
【基金】：國家自然科學基金資助項目(81360026)
【分類號】：R54

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