目的用�；撬幔═aurine）對重癥急性胰腺炎（severe acute pancreatitis ,SAP）大鼠進(jìn)行預(yù)處理,采用聯(lián)合酶原位灌注法分離枯否細(xì)胞（Kupffer cells,KCs）,檢測KCs中P38-絲裂原蛋白激酶（P38 mitogen activated protein kinase, P38MAPK）的蛋白量和其活化水平以及核因子-κB（Nuclear factor-kappaB, NF-κB）的活性,探討�；撬釋χ匕Y急性胰腺炎大鼠KCs內(nèi)P38MAPK的影響以及對分泌促炎細(xì)胞因子的作用。方法48只雄性SD大鼠采用完全隨機(jī)法分為:①假手術(shù)對照組（sham operation,SO）;②重癥急性胰腺炎組（SAP）;③�；撬犷A(yù)處理組（Tau）。SAP模型通過胰膽管逆行注射5%牛磺膽酸鈉誘導(dǎo),造模前Tau組分別于24h和12h從尾靜脈內(nèi)注入�；撬幔�300mmol/L,1.0ml /100g）,其它組注入等量的生理鹽水。假手術(shù)或造模后分別于12h和24h處死動物,檢測其血清中AST、ALT和AMS含量;然后采用聯(lián)合酶原位灌注法分離KCs,采用蛋白免疫印跡法和免疫細(xì)胞化學(xué)... 

【文章來源】：重慶醫(yī)科大學(xué)重慶市

【文章頁數(shù)】：55 頁

【學(xué)位級別】：碩士

【部分圖文】：

各時(shí)間點(diǎn)大鼠血清中AST水平的變化

圖 1-1：各時(shí)間點(diǎn)大鼠血清中 AST 水平的變化注： ﹡與 SO 組比較，P<0.01； ﹟與 SAP 組比較，P<0.05。Fig 1-1: The change of AST at every time in three group rats﹡P<0.01 vs SO group; ﹟P<0.05 vs SAP group.

圖 1-3：各時(shí)間點(diǎn)大鼠血清中AMS水平的變化注： ﹡與 SO 組比較，P<0.01； ﹟與 SAP 組比較，P<0.05。Fig 1-3 :The change of AMS at every time in three group rats﹡P<0.01 vs SO group; ﹟P<0.05 vs SAP group.大鼠KCs中P38MAPK的活化情況2所示：雖然三組大鼠各時(shí)間點(diǎn)KCs中相對的P38MAPK的蛋白量.05），但各時(shí)間點(diǎn)SAP組大鼠KCs中P38MAPK明顯被活化為，其明顯增高，與SO組比較，差異顯著(P<0.01)。用�；撬犷A(yù)處理后大下降，與SAP組相比，差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。 2 KCs 中 P38MAPK 的蛋白量及磷酸化水平(P38MAPK/β-actin) ( )Tab2 The proteinum and phosphorylation of P38MAPK in KCs( ) SO 組 SAP 組 Tau 組12h 24h 12h 24h 12h 24h0.8399± 0.8447± 0.7539± 0.6021± 0.7506± 0.8

【參考文獻(xiàn)】：
期刊論文
[1]Dynamic changes and mechanism of intestinal endotoxemia in partially hepatectomized rats[J]. Cui-Ping Xu,Juan Liu,Jin-Chun Liu,Department of Digestive Diseases,First Clinical College,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China De-Wu Han,Yang Zhang,Yuan-Chang Zhao,Institute of Hepatology,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China.  World Journal of Gastroenterology. 2007(26)
[2]急性壞死性胰腺炎時(shí)枯否細(xì)胞吞噬功能的實(shí)驗(yàn)研究[J]. 張海燕,劉立新,韓德五.  山西醫(yī)科大學(xué)學(xué)報(bào). 2007(05)
[3]�；撬釋Υ笫笠浦惨认偃毖俟嘧p傷的保護(hù)作用[J]. 張克忠,劉永鋒,程穎,劉樹榮,王鳳山,石蕊,趙寧.  世界華人消化雜志. 2007(12)
[4]Role of Kupffer cells in acute hemorrhagic necrotizing pancreatitis-associated lung injury of rats[J]. Hong-Bin Liu Dong-Hua Li,Department of Pharmacology,Tianjin Institute of Acute Abdominal Diseases,Tianjin 300100,ChinaNai-Qiang Cui Chang Chen,Second Department of Surgery Tianiin Institute of Acute Abdominal Diseases,Tianjin 300100,China.  World Journal of Gastroenterology. 2006(03)
[5]Pathogenesis of acute lung injury in rats with severe acute pancreatitis[J]. Xue-Min Liu, Jun Xu and Zi-Fa Wang Department of Hepatobiliary Surgery, First Hospital, Xi’an Jiaotong University, Xi’an 710061 , China.  Hepatobiliary & Pancreatic Diseases International. 2005(04)
[6]枯否細(xì)胞在大鼠重癥急性胰腺炎肺損傷中的作用[J]. 黎冬暄,田伏洲,李紅,李旭,向珂,羅皓.  中華外科雜志. 2005(03)
[7]Expression of toll-like receptor 4 and MD-2 gene and protein in Kupffer cells after ischemia-reperfusion in rat liver graft[J]. Yong Peng Jian-PJng Gong Chang-An Liu Xu-Hong Li Ling Gan Shou-Bai Li Department of Hepatobiliary Surgery,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China.  World Journal of Gastroenterology. 2004(19)
[8]胰彈性蛋白酶對內(nèi)毒素誘導(dǎo)的肝枯否細(xì)胞TNF-α、IL-1β表達(dá)的影響[J]. 黎冬暄,田伏洲,李紅,李旭,羅皓,向珂.  解放軍醫(yī)學(xué)雜志. 2004(09)
[9]p38MAPK在重癥急性胰腺炎大鼠枯否細(xì)胞分泌促炎細(xì)胞因子中的作用[J]. 任洪波,李兆申,許國銘,屠振興,賈一韜,施新崗,龔燕芳.  中國病理生理雜志. 2003(07)
[10]Role of NF-kB in multiple organ dysfunction during acute obstructive cholangitis[J]. Bin Tu Jian-Ping Gong Hu-Yi Feng Chuan-Xin Wu Yu-Jun Shi Xu-Hong Li Yong Peng Chang-An Liu Sheng-Wei Li Department of General Surgery,the Second College of Clinical Medicine & the Second Affiliated Hospital of Chongqing University of Medical Science,74 Linjiang Road,Chongqing 400010,China.  World Journal of Gastroenterology. 2003(01)



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