本文關(guān)鍵詞： 雌激素類 炎性腸疾病 雌激素受體α 促炎效應(yīng)　出處：《第二軍醫(yī)大學(xué)學(xué)報(bào)》2016年04期 　論文類型：期刊論文

【摘要】：目的探討雌激素影響大鼠炎癥性腸病(inflammatory bowel disease,IBD)的具體機(jī)制。方法通過(guò)30%的2,4,6-三硝基苯磺酸(TNBS)乙醇溶液誘導(dǎo)建立大鼠IBD模型,通過(guò)測(cè)定體質(zhì)量、疾病活動(dòng)指數(shù)(disease activity index,DAI)評(píng)分、結(jié)直腸長(zhǎng)度、髓過(guò)氧化物酶(MPO)濃度、H-E染色指標(biāo),以確定IBD建模成功。致炎后的IBD鼠,分別應(yīng)用生理鹽水(500μL)、雌激素(1mg/kg,溶于500μL生理鹽水)、雌激素受體α(ERα)特異性激動(dòng)劑PPT(3mg/kg,溶于500μL生理鹽水)、雌激素(1mg/kg,溶于500μL生理鹽水)+ERα特異性拮抗劑MPP(1mg/kg,溶于500μL生理鹽水)作處理,觀察大鼠炎癥變化。結(jié)果 TNBS誘導(dǎo)大鼠IBD模型成功建立,大鼠體質(zhì)量降低、DAI評(píng)分增大、MPO值增加、腸道炎癥明顯。與生理鹽水處理組比較,用雌激素或PPT處理后加重了IBD大鼠炎癥:雌激素干預(yù)后IBD大鼠體質(zhì)量降低、DAI評(píng)分增大、結(jié)直腸長(zhǎng)度縮短、腸道炎癥加重;PPT干預(yù)后IBD大鼠體質(zhì)量降低、DAI評(píng)分增大、結(jié)直腸長(zhǎng)度縮短、腸道炎癥加重。而MPP逆轉(zhuǎn)了雌激素的促炎效應(yīng):與單獨(dú)注射雌激素組比較,MPP干預(yù)后大鼠體質(zhì)量增加、DAI評(píng)分降低、結(jié)直腸長(zhǎng)度增加。結(jié)論雌激素促進(jìn)TNBS誘導(dǎo)的IBD的發(fā)生發(fā)展,并且這種作用可能是通過(guò)ERα來(lái)完成的。
[Abstract]:Objective to investigate the mechanism of estrogen affecting inflammatory bowel disease in rats. The rat IBD model was established by ethanol solution of 6-trinitrobenzene sulfonic acid (TNBS). The body mass and disease activity index (activity index) were measured. DAI) score, colorectal length, myeloperoxidase (MPO) concentration and H-E staining were used to determine the success of IBD modeling in IBD mice after inflammation. PPT(3mg/kg, a specific agonist of estrogen receptor 偽 -ER 偽, was treated with normal saline 500 渭 L, estrogen 1 mg / kg, dissolved in 500 渭 L normal saline. MPP(1mg/kg, a specific antagonist of ER 偽, was soluble in 500 渭 L saline, 1 mg / kg estrogen and 500 渭 L saline. Rats were treated with 500 渭 L saline to observe the changes of inflammation. Results TNBS induced rat IBD model was successfully established, and the decrease of body mass and the increase of Dai score increased. Compared with normal saline treatment group, the inflammation of IBD rats was aggravated after estrogen or PPT treatment. After estrogen intervention, the body mass of IBD rats decreased and the Dai score increased. The length of colon and rectum was shortened and the intestinal inflammation was aggravated. After PPT intervention, the body mass of IBD rats decreased and the Dai score increased, the length of colon and rectum shortened and the intestinal inflammation aggravated. MPP reversed the pro-inflammatory effect of estrogen: compared with the group injected with estrogen alone. After the intervention of MPP, the weight of rats increased and the score of Dai decreased and the length of colon and rectum increased. Conclusion estrogen can promote the development of IBD induced by TNBS. And this action may be done through ER 偽.
【作者單位】： 第二軍醫(yī)大學(xué)基礎(chǔ)醫(yī)學(xué)部實(shí)驗(yàn)教學(xué)中心;
【基金】：國(guó)家自然科學(xué)基金(31471103)~~
【分類號(hào)】：R574
【正文快照】： 炎癥性腸病(inflammatory bowel disease,IBD)是一種多種病因引起的、異常免疫介導(dǎo)的腸道慢性及復(fù)發(fā)性炎癥,具有慢性遷延、難以治愈以及終生復(fù)發(fā)的傾向等特點(diǎn),潰瘍性結(jié)腸炎(ulcerativecolitis,UC)和克羅恩病(Crohn disease,CD)是其主要的疾病類型。其病因和發(fā)病機(jī)制十分復(fù)雜,

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