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甘蔗葉多糖對鼻咽癌細胞株CNE2的體外抑制作用研究

發(fā)布時間:2018-07-08 09:02

  本文選題:甘蔗葉多糖 + CNE_2細胞 ; 參考:《廣西醫(yī)科大學》2012年碩士論文


【摘要】:目的:研究甘蔗葉多糖對鼻咽癌CNE_2細胞株的體外抑制作用,并初步探討其作用機制。 方法:采用熱水浸提及DEAE-纖維素層析柱法提純甘蔗葉多糖,以MTT法觀察其體外抗腫瘤活性,透射電子顯微鏡觀察凋亡細胞的超微結構,采用流式細胞術檢測腫瘤細胞凋亡率,實時熒光定量PCR法檢測凋亡相關基因Bax、Bcl-2的變化。 結果:濃度為10ug/ml、20ug/ml、40ug/ml、80ug/ml、160ug/ml的甘蔗葉多糖作用后,CNE_2細胞抑制率及凋亡率均明顯增加,且呈濃度依賴性。電鏡下可見160ug/ml濃度的甘蔗葉多糖可使CNE_2細胞出現(xiàn)凋亡小體。熒光定量PCR技術測定Bax在CNE_2細胞中表達上調,并隨著甘蔗葉多糖作用濃度的增加而升高;Bcl-2則在CNE_2細胞中表達下調,,且隨著甘蔗葉多糖作用濃度的增加而降低。 結論:甘蔗葉多糖能明顯抑制人鼻咽癌CNE_2細胞增殖,誘導細胞凋亡,可能通過提高Bax表達及降低Bcl-2的表達,從而發(fā)揮抗腫瘤作用。
[Abstract]:Aim: to study the inhibitory effect of sugarcane leaf polysaccharide on CNE2 cell line of nasopharyngeal carcinoma (NPC) in vitro and to explore its mechanism. Methods: the polysaccharides from sugarcane leaves were purified by hot water extraction and DEAE- cellulose chromatography. The in vitro antitumor activity was observed by MTT assay, the ultrastructure of apoptotic cells was observed by transmission electron microscope, and the apoptosis rate of tumor cells was detected by flow cytometry. The changes of apoptosis-related gene Baxon Bcl-2 were detected by real-time fluorescence quantitative PCR. Results: the inhibition rate and apoptosis rate of CNE2 cells were significantly increased after the treatment of sugar cane leaf polysaccharides at the concentration of 10ugrml / ml 20ugr / ml 40ugrml / ml ~ 80ugr / ml sugarcane leaf polysaccharide in a concentration-dependent manner. The inhibitory rate and apoptosis rate of CNE2 cells were increased in a concentration-dependent manner. Under electron microscope, 160ug/ml concentration of polysaccharides from sugarcane leaves could make CNE2 cells appear apoptotic bodies. The expression of Bax was up-regulated in CNE2 cells by fluorescence quantitative PCR, and the expression of Bcl-2 was down-regulated in CNE2 cells with the increase of polysaccharide concentration in sugarcane leaves, and decreased with the increase of polysaccharide concentration in sugarcane leaves. Conclusion: polysaccharide from sugarcane leaves can significantly inhibit the proliferation of CNE2 cells and induce apoptosis, which may play an anti-tumor role by increasing the expression of Bax and decreasing the expression of Bcl-2.
【學位授予單位】:廣西醫(yī)科大學
【學位級別】:碩士
【學位授予年份】:2012
【分類號】:R739.63

【參考文獻】

相關期刊論文 前10條

1 位鴻,李文香,王艷英;中藥抗腫瘤作用研究進展[J];長春中醫(yī)學院學報;2001年03期

2 張小梅;黃芪多糖的免疫調節(jié)作用及抗腫瘤作用研究進展[J];大連大學學報;2003年06期

3 商澎,楊鐵虹,賈敏,梅其炳,朱德生,趙文明,趙德化;當歸多糖AP-0對小鼠移植性腫瘤的抑制作用[J];第三軍醫(yī)大學學報;2001年11期

4 潘冉;香菇多糖臨床應用研究進展及香菇的藥用價值[J];海峽藥學;2005年04期

5 于恩廣;王龍云;;蘆薈多糖的抗腫瘤作用[J];黑龍江醫(yī)藥;2004年02期

6 季宇彬,高世勇,成秉辰;海藻多糖抗腫瘤作用的實驗研究[J];哈爾濱商業(yè)大學學報(自然科學版);2001年04期

7 陳 群,劉家昌;人參多糖、黃芪多糖、枸杞多糖的研究進展[J];淮南師范學院學報;2001年02期

8 劉娟妮;王雪青;魏丹;郅琪;李晨韻;龐廣昌;;螺旋藻多糖的積累與抗腫瘤作用的研究[J];海洋通報;2009年06期

9 呂曉英,曾令福,蔡亞南,李由,劉新華;紅毛五加多糖-Ⅰ誘導體外人胃癌細胞凋亡的研究[J];華西醫(yī)科大學學報;2002年02期

10 顧云燕;吳瑾瑾;;枸杞多糖抗腫瘤作用實驗研究進展[J];浙江臨床醫(yī)學;2007年09期

相關碩士學位論文 前1條

1 李小平;紅棗多糖提取工藝研究及其生物功能初探[D];陜西師范大學;2004年



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