發(fā)布時間：2018-03-31 09:36

  本文選題：中耳炎　切入點：流感嗜血桿菌　出處：《重慶醫(yī)科大學》2017年碩士論文

【摘要】：背景中耳炎是兒童時期最常見的感染性疾病之一,多種基因均參與其發(fā)病過程。目前已有關(guān)于小鼠中耳炎的基因芯片研究,但都是基于流感嗜血桿菌所致的中耳炎,對肺炎鏈球菌引起的中耳炎的基因表達譜芯片研究至今尚未開展過。并且目前對中耳炎研究主要傾向于傳統(tǒng)單基因方面,顯然已不能滿足當前系統(tǒng)生物學研究的需要。因此,本研究一方面繪制出流感嗜血桿菌所致中耳炎的差異基因互作網(wǎng)絡(luò)圖,篩選出其主要網(wǎng)絡(luò)中心節(jié)點,另一方面研究肺炎鏈球菌性小鼠急性中耳炎時所涉及的基因改變。方法用生物信息學軟件對GEO數(shù)據(jù)庫中已有流感嗜血桿菌中耳炎相關(guān)基因芯片原始數(shù)據(jù)進行分析,繪制差異基因網(wǎng)絡(luò);用鼓膜穿刺法建立小鼠肺炎鏈球菌中耳炎模型,并進行m RNA表達譜生物芯片分析,篩選中耳與內(nèi)耳的差異基因。結(jié)果流感嗜血桿菌中耳炎表達譜芯片(GSE66141)經(jīng)STRING在線工具、Cytoscape軟件分析后得到其基因網(wǎng)絡(luò)圖;肺炎鏈球菌中耳炎表達譜芯片結(jié)果顯示中耳中702個基因上調(diào),250個基因下調(diào);內(nèi)耳中上調(diào)基因140個,下調(diào)基因171個。結(jié)論本研究的結(jié)果可能為中耳炎的分子機制提供更好的了解。
[Abstract]:Background Otitis media is one of the most common infectious diseases in childhood.Up to now, there has been gene chip research on mouse otitis media, but it is based on Haemophilus influenzae. The gene expression microarray of Streptococcus pneumoniae induced otitis media has not been carried out yet.At present, the research on otitis media is mainly focused on the traditional single gene, which obviously can not meet the needs of the current system biology research.Therefore, on the one hand, the differential gene interaction network of Haemophilus influenzae induced otitis media was drawn, and its main network center nodes were screened. On the other hand, the genetic changes involved in acute otitis media induced by Streptococcus pneumoniae in mice were studied.Methods the original data of Haemophilus influenzae otitis media related gene chip in GEO database were analyzed with bioinformatics software, and the differential gene network was drawn, and the mouse model of Streptococcus pneumoniae otitis media was established by tympanic membrane puncture.The differential genes of middle ear and inner ear were screened by biochip analysis of m RNA expression profile.Results the gene network of Haemophilus influenzae tympanitis expression microarray (GSE66141) was analyzed by STRING online tool Cytoscape software, and the results showed that 702 genes were up-regulated and 250 genes down-regulated in the middle ear of Streptococcus pneumoniae.There were 140 up-regulated genes and 171 down-regulated genes in the inner ear.Conclusion the results of this study may provide a better understanding of the molecular mechanism of otitis media.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R764.21;R-332

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本文編號：1690157