本文選題：右美托咪定 + microRNAs　； 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：目的:右美托咪定的應用可能在心臟外科手術中具有有利影響。MicroRNAs是一種有基因表達調控功能的非編碼RNA。本研究旨在研究MicroRNAs在右美托咪定心臟保護中的作用。方法:本研究入選了六只大鼠并分成了兩組:右美托咪定組和對照組,右美托咪定組大鼠腹腔注射右美托咪定(100ug/Kg),對照組大鼠腹腔注射同等劑量的生理鹽水。給射后30分鐘,麻醉下取出大鼠標記后儲存在液氮中。所有樣本的microRNAs表達量通過microRNA基因芯片檢測。結果:兩組大鼠心臟microRNA表達量存在差異,其中5 個microRNA(mo-miRNA-X2,rno-miRNA-X3,rno-miRNA-X4,rno-miRNA-X5 and rno-miRNA-X6)的表達量上調的,1個microRNA的表達量是下調的。對照組和右美托咪定組的大鼠的microRNAs表達量通過T檢驗進行對比,P小于0.05(P0.05),這表明上述結果具有統(tǒng)計學意義。結論:右美托咪定可引起心臟microRNA產生差異表達,這些差異表達的microRNA可能與心臟保護有關。
[Abstract]:Objective: the application of dexmetomidine may have a beneficial effect in cardiac surgery. MicroRNAs are a kind of non-coding RNAs with the function of gene expression regulation. The purpose of this study was to investigate the role of MicroRNAs in the cardiac protection of dexmetomidine. Methods: six rats were divided into two groups: right metoimidine group and control group, right metoimidine group rats were injected intraperitoneally with dexmetoimidine 100ugr / KgG, and control rats were intraperitoneally injected with the same dose of normal saline. 30 minutes after injection, the rats were taken out under anesthesia and stored in liquid nitrogen. The expression of microRNAs in all samples was detected by microRNA gene chip. Results: there was significant difference in the expression of microRNA between the two groups. The expression of 5 microRNAs mo-miRNA-X2nrno-miRNA-X3rno-miRNA-X4 rno-miRNA-X5 and rno-miRNA-X6 was up-regulated, and one microRNA was down-regulated. The expression of microRNAs in the control group and dexmetidine group was compared by T test (P < 0.05), which indicated that the above results were statistically significant. Conclusion: dexmetomidine can induce differential expression of microRNA in the heart, and these differential expressions of microRNA may be related to cardiac protection.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R614

【參考文獻】

相關期刊論文 前1條

1 姜翠翠;夏滿莉;王敏;陳士票;;右美托咪定預處理減輕離體大鼠心臟缺血/再灌注損傷的線粒體相關機制[J];浙江大學學報(醫(yī)學版);2013年03期

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本文編號：1806840