本文關(guān)鍵詞： 不完全性脊髓損傷 減重步行訓(xùn)練 腳橋核 型囊泡谷氨酸轉(zhuǎn)運(yùn)體　出處：《中國康復(fù)醫(yī)學(xué)雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的:探索減重步行訓(xùn)練對(duì)不完全脊髓損傷大鼠腳橋核可塑性的影響。方法:將雌性SD大鼠24只分為減重步行訓(xùn)練組、未訓(xùn)練組和假手術(shù)組,每組8只,用美國NYU脊髓沖擊損傷儀制作大鼠T10脊髓不完全損傷模型。采用PET-CT、免疫熒光染色及BBB評(píng)分觀察減重步行訓(xùn)練對(duì)不完全脊髓損傷(iSCI)大鼠中腦運(yùn)動(dòng)區(qū)(MLR)腳橋核可塑性變化和后肢運(yùn)動(dòng)功能的改善。結(jié)果:減重步行訓(xùn)練組大鼠的后肢運(yùn)動(dòng)功能BBB評(píng)分在造模后4周、7周兩個(gè)時(shí)間點(diǎn)的分值均較未訓(xùn)練組高(P0.05)。假手術(shù)組大鼠在造模后1周、4周、7周3個(gè)時(shí)間點(diǎn)的BBB評(píng)分值均明顯高于訓(xùn)練組和未訓(xùn)練組(P0.01)。雖然訓(xùn)練組18F-氟脫氧葡萄糖(18F-FDG)的標(biāo)準(zhǔn)攝取值(SUV)的平均值較未訓(xùn)練組增高,而且假手術(shù)組的SUV值最高,但三組SUV值的差異不具有顯著性意義(P0.05)。神經(jīng)元特異性核抗原(NeuN)免疫熒光染色檢測顯示訓(xùn)練組、未訓(xùn)練組、假手術(shù)組三組間大鼠腳橋核的神經(jīng)元數(shù)量差異無顯著性意義(P0.05),而腳橋核2型囊泡谷氨酸轉(zhuǎn)運(yùn)體(VGLUT2)的積分光密度值(IOD)比較,假手術(shù)組IOD表達(dá)最高,訓(xùn)練組較未訓(xùn)練組明顯增強(qiáng)(P0.01)。結(jié)論:減重步行訓(xùn)練能夠明顯提高iSCI大鼠后肢運(yùn)動(dòng)功能及其腳橋核中谷氨酸能信號(hào)傳遞,而且iSCI后大鼠中腦腳橋核的整體代謝及神經(jīng)元數(shù)量無明顯改變。因此認(rèn)為腳橋核的谷氨酸表達(dá)增強(qiáng)是減重步行訓(xùn)練使iSCI大鼠后肢步行功能改善的原因之一。減重步行訓(xùn)練能使腳橋核發(fā)生神經(jīng)遞質(zhì)表達(dá)層面的可塑性變化。
[Abstract]:Objective: to explore the effect of weight-loss walking training on the plasticity of pontine nucleus in rats with incomplete spinal cord injury. Methods: Twenty-four female Sprague-Dawley rats were divided into weight-loss walking training group, untrained group and sham-operated group with 8 rats in each group. The model of incomplete injury of T10 spinal cord in rats was made by NYU spinal cord impact injury instrument. The plasticity of the pontine nucleus of the foot of the midbrain motor area in rats with incomplete spinal cord injury was observed by PET-CT, immunofluorescence staining and BBB score. Results: the BBB score of hind limb motor function in the weight-loss walking training group was higher than that in the untrained group at 4 weeks and 7 weeks after modeling (P 0.05). The BBB scores of the three time points at 4 weeks and 7 weeks were significantly higher than those of the training group and the untrained group (P 0.01), although the standard uptake of 18F-FDG in the training group was higher than that in the untrained group, although the average value of 18F-FDG in the training group was higher than that in the untrained group. The SUV value of the sham operation group was the highest, but the difference of SUV value among the three groups was not significant (P 0.05). The detection of neuron specific nuclear antigen (Neun) immunofluorescence staining showed that the training group, the untrained group, the training group, and the untrained group, There was no significant difference in the number of neurons in the pontine nucleus between the three groups of sham-operated rats, but the expression of IOD was the highest in the sham operation group compared with the integral optical density (IOD) of type 2 vesicular glutamate transporter (VGLUT2). Conclusion: weight-loss walking training can significantly improve the motor function of hind limbs and glutaminergic signal transduction in the pontine nucleus of the feet of iSCI rats. There was no significant change in the metabolism and the number of neurons in the pontine nucleus after iSCI. Therefore, it is considered that the increase of glutamate expression in the pontine nucleus of the foot is one of the reasons for the improvement of the walking function of the hind limbs of iSCI rats after weight-loss walking training. Walking training can change the plasticity of neurotransmitter expression in the nucleus of the foot.
【作者單位】： 南方醫(yī)科大學(xué)第二臨床醫(yī)學(xué)院;陸軍總醫(yī)院骨科研究所;解放軍醫(yī)學(xué)院;空軍總醫(yī)院康復(fù)科;
【基金】：國家自然科學(xué)基金應(yīng)急管理項(xiàng)目(81650013);國家自然科學(xué)基金面上項(xiàng)目(81171862)
【分類號(hào)】：R651.2

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