本文關(guān)鍵詞： 氯化鎘 骨肉瘤細(xì)胞 細(xì)胞凋亡 細(xì)胞周期　出處：《實(shí)用癌癥雜志》2016年11期 　論文類型：期刊論文

【摘要】：目的通過觀察氯化鎘誘導(dǎo)的骨肉瘤細(xì)胞凋亡與細(xì)胞特異性周期阻滯及caspase活化的影響,闡明氯化鎘誘導(dǎo)的細(xì)胞凋亡效應(yīng)及其分子機(jī)制。方法應(yīng)用Annexin V/PI和API等標(biāo)記,流式細(xì)胞儀對(duì)人骨腫瘤細(xì)胞凋亡及周期特異性的檢測,運(yùn)用RT-PCR的方法檢查Caspase-3、Caspase-9活化情況。結(jié)果處于靜止期的外周血對(duì)氯化鎘凋亡誘導(dǎo)不敏感,而G_1期氯化鎘誘導(dǎo)骨肉瘤細(xì)胞出現(xiàn)了明顯的細(xì)胞凋亡。氯化鎘誘導(dǎo)的細(xì)胞凋亡主要是在細(xì)胞周期的G_1期發(fā)生。觀察組采用氯化鎘凋亡誘導(dǎo),對(duì)照組未做處理,結(jié)果顯示觀察組的Caspase-3、Caspase-9 mRNA的表達(dá)值顯著高于對(duì)照組。結(jié)論氯化鎘誘導(dǎo)的細(xì)胞凋亡與骨肉瘤細(xì)胞的細(xì)胞周期相關(guān),存在G_1期細(xì)胞周期阻滯的周期特異性,且此過程中發(fā)生了caspase活化,參與細(xì)胞凋亡。
[Abstract]:Objective to investigate the effects of cadmium chloride on apoptosis, cell specific cycle arrest and caspase activation in osteosarcoma cells, and to elucidate the effect of cadmium chloride on apoptosis and its molecular mechanism. Flow cytometry was used to detect the apoptosis and cycle specificity of human bone tumor cells. The activation of Caspase-3 and Caspase-9 was detected by RT-PCR. Results the peripheral blood in stationary phase was not sensitive to the apoptosis induced by cadmium chloride. The apoptosis of osteosarcoma cells was induced by cadmium chloride at the first phase of Gal 1. The apoptosis of osteosarcoma cells induced by cadmium chloride mainly occurred in the first phase of the cell cycle. The observation group was induced by cadmium chloride apoptosis, but the control group was not treated. The results showed that the expression of Caspase-3 and Caspase-9 mRNA in the observation group was significantly higher than that in the control group. Conclusion the apoptosis induced by cadmium chloride is related to the cell cycle of osteosarcoma cells. Participate in apoptosis.
【作者單位】： 湖北省武漢市普仁醫(yī)院;
【分類號(hào)】：R738

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 歐陽彩群;葉卓明;曾艷飛;;亞硒酸鈉拮抗氯化鎘染毒大鼠肝、腎細(xì)胞損傷的研究[J];浙江預(yù)防醫(yī)學(xué);2012年07期

2 魏雪濤,薛彬;氯化鎘對(duì)小鼠腹腔巨噬細(xì)胞功能的影響[J];中國藥理學(xué)與毒理學(xué)雜志;1997年02期

3 王曉麗,李永安,馮彪,凌翎,李志超,郭麗,范洪學(xué);氯化鎘致癌機(jī)理的研究[J];中國公共衛(wèi)生學(xué)報(bào);1999年02期

4 李永安,王曉麗,石愛平,馮彪,凌翎,李志超,范洪學(xué);氯化鎘對(duì)人胚肺成纖維細(xì)胞的惡性轉(zhuǎn)化作用[J];衛(wèi)生研究;2000年01期

5 魏雪濤,宋曉明,薛彬;氯化鎘對(duì)小鼠免疫細(xì)胞增殖和凋亡的影響[J];環(huán)境與職業(yè)醫(yī)學(xué);2003年06期

6 李國慶;李，

本文編號(hào)：1515173