本文關(guān)鍵詞： 骨橋蛋白質(zhì) 骨關(guān)節(jié)炎 軟骨細(xì)胞 細(xì)胞增殖 組織工程 組織構(gòu)建 骨橋蛋白 RNA干擾 慢病毒轉(zhuǎn)染 MTT BrdU　出處：《中國組織工程研究》2017年24期 　論文類型：期刊論文

【摘要】：背景:骨橋蛋白是一種細(xì)胞外多功能基質(zhì)糖蛋白,已被證實(shí)參與調(diào)控骨關(guān)節(jié)炎軟骨細(xì)胞外基質(zhì)成分的合成分解代謝,但對于骨關(guān)節(jié)炎軟骨細(xì)胞本身增殖能力的影響,報(bào)道較少。目的:觀察骨橋蛋白對人膝骨關(guān)節(jié)炎軟骨細(xì)胞增殖能力的影響。方法:所有軟骨標(biāo)本均取自湘雅醫(yī)院于2012年1至6月因骨關(guān)節(jié)炎行膝關(guān)節(jié)置換患者,分離培養(yǎng)骨關(guān)節(jié)炎軟骨細(xì)胞,分別予以骨橋蛋白干預(yù)、骨橋蛋白sh RNA慢病毒轉(zhuǎn)染、空白對照及Con-shR NA轉(zhuǎn)染等處理,利用MTT及BrdU法檢測細(xì)胞的增殖能力變化。結(jié)果與結(jié)論:(1)骨橋蛋白shR NA慢病毒對骨關(guān)節(jié)炎軟骨細(xì)胞轉(zhuǎn)染率為80%;(2)與對照組相比,骨橋蛋白干預(yù)可明顯上調(diào)骨關(guān)節(jié)炎軟骨細(xì)胞吸光度值(P0.05),而骨橋蛋白sh RNA慢病毒干擾組吸光度值則顯著下降(P0.05);(3)骨橋蛋白shR NA轉(zhuǎn)染后,骨橋蛋白的表達(dá)水平顯著下降(P0.05);(4)結(jié)果表明骨橋蛋白可促進(jìn)骨關(guān)節(jié)炎軟骨細(xì)胞增殖,有望成為治療骨關(guān)節(jié)炎的新靶點(diǎn)。
[Abstract]:Background: osteopontin is an extracellular multifunctional matrix glycoprotein, which has been proved to be involved in the regulation of the synthesis and catabolism of extracellular matrix components in osteoarthritis chondrocytes, but the effect of osteopontin on the proliferation of osteoarthritis chondrocytes. Objective: to observe the effect of osteopontin on the proliferation of chondrocytes in human knee osteoarthritis. Methods: all cartilage samples were collected from patients undergoing knee arthroplasty in Xiangya Hospital from 2012 to June. Osteoarthritis chondrocytes were isolated and cultured and treated with osteopontin intervention, osteopontin sh RNA lentivirus transfection, blank control and Con-shR na transfection. Results and conclusion the transfection rate of osteopontin shR na lentivirus to osteoarthritis chondrocytes was 802) compared with the control group. Osteopontin intervention significantly upregulated the absorbance of osteoarthritis chondrocytes and decreased the absorbance value of osteopontin sh RNA lentivirus interference group after transfection of osteopontin shR na. The results showed that osteopontin could promote the proliferation of osteoarthritis chondrocytes and might become a new target for the treatment of osteoarthritis.
【作者單位】： 深圳市人民醫(yī)院骨關(guān)節(jié)科暨南大學(xué)第二臨床醫(yī)學(xué)院;中南大學(xué)湘雅醫(yī)院骨科;
【基金】：深圳市科技創(chuàng)新項(xiàng)目(JCYJ20150403101028191)~~
【分類號】：R684.3

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