近年來,陽離子聚合物、陽離子脂質(zhì)體等非病毒載體在基因遞送中的應(yīng)用越來越受到人們的關(guān)注.然而,血清的存在會降低它們的轉(zhuǎn)染效率,從而阻礙它們在臨床中的使用.因此,非常有必要開發(fā)能抵抗血清干擾的非病毒基因載體.本工作合成了一種新型的鋅離子配位的脂質(zhì),其與1, 2-二油酰-SN-甘油-3-磷酰乙醇胺脂質(zhì)(DOPE)共自組裝形成陽離子脂質(zhì)體(Zn-DTAc脂質(zhì)體).實(shí)驗(yàn)結(jié)果表明,Zn-DTAc脂質(zhì)體可以有效地壓縮質(zhì)粒形成納米復(fù)合物.即使在50%胎牛血清存在的情況下,該復(fù)合物也能進(jìn)行有效的細(xì)胞內(nèi)化和內(nèi)涵體逃逸,并實(shí)現(xiàn)高效的基因轉(zhuǎn)染.

【文章頁數(shù)】：9 頁

【文章目錄】：
1 Introduction
2 Materials and methods
    2.1 Materials
    2.2 Synthesis
        2.2.1 Synthesis of DPA-Tyramine
        2.2.2 Synthesis of DPA-Tyramine-Ac14
        2.2.3 Synthesis of Zn-DTAc lipid
    2.3 Preparation of Zn-DTAc liposomes
    2.4 Preparation and characterization of Zn-DTAc/pDNA complexes
    2.5 Agarose gel electrophoresis
    2.6 Cell culture
    2.7 In vitro gene transfection
    2.8 MTT cytotoxicity assay
    2.9 Cellular uptake
    2.10 Confocal imaging
3 Results and discussions
    3.1 Synthesis and characterization of materials
    3.2 Gene transfection in vitro
    3.3 Cytotoxicity evaluation
4 Conclusions
Conflict of interest



本文編號：4008176