【摘要】：目的： 應(yīng)用異位成骨實(shí)驗(yàn)檢測不同方法制備的脫礦牙本質(zhì)基質(zhì)(Demineralized Dental Matrix,DDM)的骨誘導(dǎo)性及生物相容性,為下一階段DDM骨誘導(dǎo)機(jī)理的深入研究做基礎(chǔ)。 方法： 采用統(tǒng)一的取材標(biāo)準(zhǔn)收集各類離體牙,應(yīng)用目前國外兩種常規(guī)制備方法制備脫礦牙本質(zhì)基質(zhì)(DDM)。然后在4只Beagle犬雙側(cè)股部肌肉建立DDM肌內(nèi)異位植入模型,實(shí)驗(yàn)根據(jù)植入材料的不同,分為A、B、 C、 D、 E五組,A組植入DDM1, B組植入DDM1’(滅活法處理的DDM1),C組植入DDM2, D組植入DDM2’(滅活法處理的DDM2),E組植入DDM3(成品DDM)。按預(yù)定位置在左側(cè)植入A、B兩組材料,右側(cè)植入C、D、E三組材料,每組4個(gè)時(shí)間位點(diǎn)。術(shù)后分別于2W、4W、6W、8W取材,行大體、組織學(xué)觀察及堿性磷酸酶活性測定,運(yùn)用SPSS17.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果： 1、大體觀察顯示：所有Beagle犬各時(shí)間點(diǎn)術(shù)后飲食、活動(dòng)均正常,無死亡,創(chuàng)口愈合良好,無紅腫、膿性滲出及創(chuàng)口裂開,一周左右創(chuàng)口完全愈合。 2、各時(shí)間點(diǎn)的取材組織觀察：所有實(shí)驗(yàn)組與對(duì)照組材料與肌肉之間結(jié)合較緊密,未見明顯的纖維囊壁形成,肌肉組織正常,未見充血水腫,無感染、壞死。 3、組織學(xué)觀察：DDM3植入比格犬股部肌袋內(nèi)發(fā)生異位成骨：8周時(shí)有類骨質(zhì)形成,其中可見小梁骨;DDM1、DDM2及DDM滅活組未見成骨發(fā)生。 4、ALP測定：組內(nèi)比較,DDM3各時(shí)間點(diǎn)ALP的差異有統(tǒng)計(jì)學(xué)意義(P0.01),其余組各時(shí)間點(diǎn)ALP的差異無統(tǒng)計(jì)學(xué)意義。組間比較,DDM3組各時(shí)間點(diǎn)ALP值均高于其他組,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。 結(jié)論： 在本實(shí)驗(yàn)條件下的觀察周期內(nèi),自制的DDM及滅活的DDM具有良好的生物相容性,但未表現(xiàn)出骨誘導(dǎo)活性。DDM3(成品DDM)具有良好的生物相容性和骨誘導(dǎo)活性。
[Abstract]:Aim: to detect the bone induction and biocompatibility of demineralized dentin matrix (Demineralized Dental Matrix,DDM prepared by different methods by ectopic osteogenesis test, and to provide a basis for further study of bone induction mechanism of DDM in the next stage. Methods: all kinds of isolated teeth were collected by unified material collection standard, and demineralized dentin matrix (DDM). Was prepared by two conventional preparation methods abroad at present. Then the model of DDM intramuscular ectopic implantation was established in the bilateral thigh muscles of 4 Beagle dogs. According to the different implantation materials, the rats were divided into five groups: group A, group B, implantation of DDM1', group DDM1), C, group DDM2, D, group DDM2', group DDM2), E, group DDM3 (finished DDM). According to the predetermined position, A and B were implanted on the left, C, D and E were implanted on the right, with 4 time sites in each group. After operation, the samples were taken at 2W, 4W, 6W, 8W, gross, histologic observation and alkaline phosphatase activity were measured, and the statistical analysis was carried out by SPSS17.0 statistical software. Results: 1. General observation showed that all Beagle dogs had normal diet, no death, good wound healing, no redness and swelling, purulent exudation and wound laceration, and the wound healed completely in about one week. 2. Observation of the tissue at each time point: all the experimental group and the control group had close binding between the material and muscle, no obvious formation of fiber capsule wall, normal muscle tissue, no hyperemia and edema, no infection and necrosis. 3. Histologic observation: ectopic osteogenesis occurred in the thigh muscle bag of Beagle dogs after implantation of DDM3: osteoid formation was found at 8 weeks, but no osteogenesis was found in DDM1,DDM2 and DDM inactivated groups. 4, ALP determination: there was significant difference in ALP at each time point of DDM3 in the group (P 0.01), but there was no significant difference in ALP in the other groups at each time point. Compared with the other groups, the ALP value of DDM3 group was higher than that of other groups at each time point, and the difference was statistically significant (P 0.01). Conclusion: during the observation period, the self-made DDM and inactivated DDM have good biocompatibility, but do not show bone induction activity. DDM3 (finished DDM) has good biocompatibility and bone induction activity.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R783.1

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