導(dǎo)電納米纖維的制備及生物學(xué)性能研究
發(fā)布時(shí)間：2018-11-18 18:43
【摘要】：納米技術(shù)發(fā)展迅速,靜電紡絲技術(shù)作為一種簡(jiǎn)單、快捷、方便的納米材料制備方法,被廣泛的用來制備各種各樣的納米材料。在生物醫(yī)學(xué)工程領(lǐng)域,該技術(shù)被用來制備各種仿生材料,以用來治療、修復(fù)或者替換人體受傷的組織或器官。聚酰胺(PA)的分子結(jié)構(gòu)中含有很多的極性酰胺基團(tuán)(-CO-NH-),它可以為細(xì)胞和生物組織提供友好的界面,具有良好的細(xì)胞相容性。使用PA的甲酸溶液進(jìn)行靜電紡絲制備PA納米纖維,探索主要的電紡參數(shù),如電壓、接收距離、濕度、溫度等,溶液參數(shù),如濃度等對(duì)PA納米纖維微觀形貌及直徑的影響。碳納米管(CNTs)是由六個(gè)碳原子組成的六邊形單元相互連接而構(gòu)成的數(shù)層的同軸圓管,其獨(dú)特的物理化學(xué)性質(zhì)使它具有良好的生物相容性、機(jī)械性能和導(dǎo)電性,使它在生物醫(yī)學(xué)領(lǐng)域有廣泛的應(yīng)用。制備PA/CNTs復(fù)合納米纖維,探索CNTs對(duì)復(fù)合纖維性能的影響。鹽酸小檗堿為黃連的提取物,抗菌譜廣,可通過人體正常代謝或吸收,對(duì)正常組織毒副作用小,以此為藥物模型制備載藥PA納米纖維、載藥PA/CNTs復(fù)合納米纖維。通過掃描電鏡(SEM)對(duì)納米纖維的微觀形貌進(jìn)行觀察;萬能材料試驗(yàn)機(jī)對(duì)納米纖維膜的力學(xué)性能進(jìn)行測(cè)試;四探針測(cè)試臺(tái)對(duì)濕潤狀態(tài)下的納米纖維膜的導(dǎo)電性進(jìn)行測(cè)試;能譜儀(EDS)對(duì)所載藥物的分布進(jìn)行測(cè)試;體外藥物釋放試驗(yàn)測(cè)試載藥納米纖維的藥物緩釋性能;固體和液體培養(yǎng)基的培養(yǎng),測(cè)試載藥納米纖維對(duì)大腸桿菌的抑菌性;將小鼠成纖維細(xì)胞(L929)接種在納米纖維膜上測(cè)試其生物相容性進(jìn)行。結(jié)果表明:隨PA濃度和電壓的增大,纖維直徑均會(huì)變粗,濃度增大時(shí)粘連減少。當(dāng)接收距離增大,纖維直徑呈減小的趨勢(shì)。當(dāng)空氣濕度較大時(shí),纖維直徑變細(xì),但是有小微球出現(xiàn),纖維之間的粘連比較嚴(yán)重。當(dāng)環(huán)境溫度比較高時(shí)(45℃),纖維形貌比較好,而比較低時(shí)(30℃),會(huì)出現(xiàn)纖維和微球的復(fù)合物。PA的甲酸溶液濃度為12wt%,電壓為18 k V,接收距離為12 cm,溫度為45℃,濕度為16%時(shí)纖維的形貌相對(duì)比較好。CNTs對(duì)復(fù)合纖維的形貌沒有明顯的影響,但是顯著的增強(qiáng)了PA/CNTs復(fù)合納米纖維的力學(xué)性能,CNTs含量的增加會(huì)使PA/CNTs復(fù)合納米纖維的直徑減小,導(dǎo)電性增強(qiáng)。當(dāng)CNTs含量為0.9wt%時(shí),PA/CNTs復(fù)合納米纖維膜的彈性模量和強(qiáng)度極限分別為802.59±15.24 MPa和36.45±1.87 MPa,電導(dǎo)率為2.9570×10-3±1.7570×10-5 S·mm-1。載藥PA納米纖維和載藥PA/CNTs復(fù)合納米纖維都具有藥物緩釋性能,后者比前者的藥物緩釋能力更好。PA納米纖維膜對(duì)大腸桿菌沒有抑菌性,固體培養(yǎng)基中不同載藥濃度的載藥PA/CNTs復(fù)合納米纖維隨著載藥量的增大對(duì)大腸桿菌抑菌效果越明顯。液體培養(yǎng)基中的載藥PA/CNTs復(fù)合納米纖維對(duì)大腸桿菌的生長有抑制作用,且載藥量越大抑制作用越明顯。MTT測(cè)試結(jié)果和L929細(xì)胞粘附、增殖的SEM照片可知,PA納米纖維、載藥PA納米纖維、載藥PA/CNTs復(fù)合納米纖維均沒有細(xì)胞毒性,有利于成纖維細(xì)胞的粘附和增殖。該導(dǎo)電復(fù)合納米纖維在神經(jīng)組織修復(fù)和引導(dǎo)神經(jīng)組織再生方面有潛在的應(yīng)用價(jià)值。
[Abstract]:Nanotechnology has developed rapidly and the electrostatic spinning technology is used as a simple, fast and convenient preparation method of the nanometer material, and is widely used for preparing a wide variety of nano materials. In the field of biomedical engineering, the technique is used to prepare various biomimetic materials for the treatment, repair or replacement of tissue or organs of a human body. Polyamide (PA) has a number of polar organic amine groups (--CO--NH--) in the molecular structure, which can provide a friendly interface for cells and biological tissues, and has good cell compatibility. The PA nano-fiber was prepared by electrospinning using the formic acid solution of PA, and the main electrospinning parameters, such as voltage, receiving distance, humidity, temperature, etc., and the influence of the parameters of the solution, such as concentration and the like on the micro-morphology and diameter of the PA nano-fiber, were explored. The carbon nano-tube (CNTs) is a coaxial circular tube of several layers composed of six carbon atoms, and its unique physical and chemical properties make it have good biocompatibility, mechanical properties and electrical conductivity, so that it has wide application in the field of biomedicine. The effect of CNTs on the properties of composite fiber was investigated. and the drug model is used for preparing the drug-carrying PA nano-fiber and the drug-carrying PA/ CNTs composite nano-fiber. The micro-morphology of the nano-fiber is observed by scanning electron microscope (SEM), the mechanical property of the nano-fiber membrane is tested by the universal material testing machine, and the conductivity of the nano-fiber membrane in the wet state is tested by the four-probe test board; the distribution of the loaded drug is tested by an energy dispersive spectrometer (EDS), the drug release test of the in-vitro drug release test is used for testing the drug sustained-release performance of the drug-carrying nano-fiber, the culture of the solid and the liquid culture medium, and the antibacterial property of the drug-carrying nano-fiber on the Escherichia coli; The mouse fibroblast (L929) was inoculated on the nanofiber membrane to test its biocompatibility. The results show that, with the increase of the concentration of PA and the voltage, the diameter of the fiber increases, and the adhesion is reduced when the concentration is increased. As the receiving distance increases, the fiber diameter tends to decrease. When the air humidity is large, the diameter of the fiber is fine, but small micro-spheres appear, and the adhesion between the fibers is severe. When the ambient temperature is relatively high (45.degree. C.), the morphology of the fibers is good, and when the comparison is low (30.degree. C.), the composite of the fibers and the microspheres will be present. The concentration of the formic acid solution of the PA is 12wt%, the voltage is 18k V, the receiving distance is 12 cm, the temperature is 45 DEG C and the humidity is 16%, the appearance of the fiber is relatively good. The influence of CNTs on the morphology of the composite fiber is not obvious, but the mechanical property of the PA/ CNTs composite nano-fiber is obviously enhanced, and the increase of the content of the CNTs can lead the diameter of the PA/ CNTs composite nano-fiber to be reduced and the electric conductivity is enhanced. When the content of CNTs is 0. 9wt%, the elastic modulus and strength limit of the PA/ CNTs composite nano-fiber membrane are 802.59-15.24MPa and 36.45-1.87MPa, and the conductivity is 2.9570-10-3-1. 7570-10-5 S 路 mm-1. The drug-carrying PA nano-fiber and the drug-carrier PA/ CNTs composite nano-fiber have the drug sustained-release property, and the latter is better than the drug sustained-release capacity of the former. The PA/ CNTs composite nano-fiber with different drug-carrier concentration in solid culture medium has no antibacterial effect on Escherichia coli, and the more obvious the antibacterial effect of the PA/ CNTs composite nano-fiber on the E. coli as the drug-carrying amount is increased. The drug-carrying PA/ CNTs composite nano-fiber in the liquid culture medium has an inhibitory effect on the growth of E. coli, and the more the drug-carrying amount is, the more obvious the inhibition effect. The results of the MTT test and the adhesion and proliferation of the L929 cells showed that the PA nano-fiber, the drug-carrying PA nano-fiber, the drug-carrying PA/ CNTs composite nano-fiber had no cytotoxicity, and the adhesion and the proliferation of the fibroblasts were facilitated. The conductive composite nano-fiber has potential application value in the repair and guidance of nerve tissue regeneration.
【學(xué)位授予單位】：太原理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08
[Abstract]:Nanotechnology has developed rapidly and the electrostatic spinning technology is used as a simple, fast and convenient preparation method of the nanometer material, and is widely used for preparing a wide variety of nano materials. In the field of biomedical engineering, the technique is used to prepare various biomimetic materials for the treatment, repair or replacement of tissue or organs of a human body. Polyamide (PA) has a number of polar organic amine groups (--CO--NH--) in the molecular structure, which can provide a friendly interface for cells and biological tissues, and has good cell compatibility. The PA nano-fiber was prepared by electrospinning using the formic acid solution of PA, and the main electrospinning parameters, such as voltage, receiving distance, humidity, temperature, etc., and the influence of the parameters of the solution, such as concentration and the like on the micro-morphology and diameter of the PA nano-fiber, were explored. The carbon nano-tube (CNTs) is a coaxial circular tube of several layers composed of six carbon atoms, and its unique physical and chemical properties make it have good biocompatibility, mechanical properties and electrical conductivity, so that it has wide application in the field of biomedicine. The effect of CNTs on the properties of composite fiber was investigated. and the drug model is used for preparing the drug-carrying PA nano-fiber and the drug-carrying PA/ CNTs composite nano-fiber. The micro-morphology of the nano-fiber is observed by scanning electron microscope (SEM), the mechanical property of the nano-fiber membrane is tested by the universal material testing machine, and the conductivity of the nano-fiber membrane in the wet state is tested by the four-probe test board; the distribution of the loaded drug is tested by an energy dispersive spectrometer (EDS), the drug release test of the in-vitro drug release test is used for testing the drug sustained-release performance of the drug-carrying nano-fiber, the culture of the solid and the liquid culture medium, and the antibacterial property of the drug-carrying nano-fiber on the Escherichia coli; The mouse fibroblast (L929) was inoculated on the nanofiber membrane to test its biocompatibility. The results show that, with the increase of the concentration of PA and the voltage, the diameter of the fiber increases, and the adhesion is reduced when the concentration is increased. As the receiving distance increases, the fiber diameter tends to decrease. When the air humidity is large, the diameter of the fiber is fine, but small micro-spheres appear, and the adhesion between the fibers is severe. When the ambient temperature is relatively high (45.degree. C.), the morphology of the fibers is good, and when the comparison is low (30.degree. C.), the composite of the fibers and the microspheres will be present. The concentration of the formic acid solution of the PA is 12wt%, the voltage is 18k V, the receiving distance is 12 cm, the temperature is 45 DEG C and the humidity is 16%, the appearance of the fiber is relatively good. The influence of CNTs on the morphology of the composite fiber is not obvious, but the mechanical property of the PA/ CNTs composite nano-fiber is obviously enhanced, and the increase of the content of the CNTs can lead the diameter of the PA/ CNTs composite nano-fiber to be reduced and the electric conductivity is enhanced. When the content of CNTs is 0. 9wt%, the elastic modulus and strength limit of the PA/ CNTs composite nano-fiber membrane are 802.59-15.24MPa and 36.45-1.87MPa, and the conductivity is 2.9570-10-3-1. 7570-10-5 S 路 mm-1. The drug-carrying PA nano-fiber and the drug-carrier PA/ CNTs composite nano-fiber have the drug sustained-release property, and the latter is better than the drug sustained-release capacity of the former. The PA/ CNTs composite nano-fiber with different drug-carrier concentration in solid culture medium has no antibacterial effect on Escherichia coli, and the more obvious the antibacterial effect of the PA/ CNTs composite nano-fiber on the E. coli as the drug-carrying amount is increased. The drug-carrying PA/ CNTs composite nano-fiber in the liquid culture medium has an inhibitory effect on the growth of E. coli, and the more the drug-carrying amount is, the more obvious the inhibition effect. The results of the MTT test and the adhesion and proliferation of the L929 cells showed that the PA nano-fiber, the drug-carrying PA nano-fiber, the drug-carrying PA/ CNTs composite nano-fiber had no cytotoxicity, and the adhesion and the proliferation of the fibroblasts were facilitated. The conductive composite nano-fiber has potential application value in the repair and guidance of nerve tissue regeneration.
【學(xué)位授予單位】：太原理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08
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