【摘要】：家蠶絲素纖維有著良好的韌性、強(qiáng)度和生物可降解性,在生物醫(yī)用材料領(lǐng)域有著廣泛的應(yīng)用。研究表明,絲素纖維機(jī)織管狀織物可以作為一種力學(xué)性能優(yōu)良的小口徑人工血管材料,然而其生物相容性并不理想。因此,本研究以絲素纖維平紋織物為原材料,采用等離子體處理技術(shù),增加材料表面極性基團(tuán)的數(shù)量,然后使用化學(xué)偶聯(lián)劑1-(3-二甲基氨基丙基)-3-乙基羰二亞胺(EDC2HCl)和N-羥基琥珀酰亞胺(NHS)將再生絲素蛋白接枝到絲素纖維材料表面。期望得到力學(xué)性能優(yōu)異、生物相容性良好的絲素纖維人工血管材料,為絲素纖維人工血管的開(kāi)發(fā)提供參考。本論文的主要內(nèi)容包括:1、采用低溫低壓等離子體技術(shù)對(duì)絲素纖維人工血管材料表面進(jìn)行處理,探究了處理氣體、時(shí)間、功率、真空度等對(duì)處理效果的影響規(guī)律;2、分別將高、低分子量再生絲素蛋白接枝到絲素纖維人工血管材料表面,探究了蛋白分子量和濃度對(duì)接枝效果的影響;3、對(duì)改性后的絲素纖維人工血管材料進(jìn)行了蛋白吸附、溶血和細(xì)胞毒性實(shí)驗(yàn),對(duì)其生物相容性進(jìn)行了評(píng)估。研究結(jié)果及主要結(jié)論如下:(1)相比于O_2、Ar兩種等離子體,He等離子體處理對(duì)絲素纖維材料的表面形態(tài)、結(jié)構(gòu)和力學(xué)性能的影響最小,而且能夠顯著提高材料表面的親水性。He等離子體處理的優(yōu)化工藝為:時(shí)間180s、功率70w、真空度50Pa。(2)高分子量再生絲素蛋白接枝后,絲素纖維人工血管材料拉伸強(qiáng)度得到提高,但材料的彈性和親水性有所下降;低分子量再生絲素蛋白接枝后,絲素纖維人工血管材料的拉伸強(qiáng)度也有所提高,而且接枝均勻性和穩(wěn)定性較好,彈性和親水性下降程度小。最終得到的優(yōu)化接枝濃度為:1%的高分子量再生絲素蛋白溶液和5%低分子量再生絲素蛋白溶液。(3)高分子量再生絲素蛋白接枝后,絲素纖維人工血管材料吸附的血漿白蛋白更多。溶血和細(xì)胞毒性實(shí)驗(yàn)結(jié)果表明,相對(duì)于低分子量再生絲素蛋白,高分子量再生絲素蛋白接枝能夠顯著地提高材料的血液和細(xì)胞相容性。綜上所述,通過(guò)He等離子體處理可以保持絲素纖維人工血管材料表面形態(tài)完整,同時(shí)力學(xué)性能損失小、親水性提高明顯。相對(duì)于低分子量再生絲素蛋白而言,接枝高分子量再生絲素蛋白可以更明顯的提高絲素纖維人工血管材料的生物相容性,但存在接枝后材料彈性降低等問(wèn)題,接枝工藝有待于進(jìn)一步完善。
[Abstract]:Silk fiber has good toughness, strength and biodegradability. It is widely used in biomedical materials. The research shows that silk fiber woven tubular fabric can be used as a small diameter artificial vascular material with excellent mechanical properties, but its biocompatibility is not ideal. Therefore, in this study, silk fiber plain fabric was used as raw material and plasma treatment technology was used to increase the number of polar groups on the surface of the material. Then the regenerated fibroin protein was grafted onto the fibroin fiber surface using the chemical coupling agents 1- (3-dimethyl aminopropyl) -3-ethyl carbonyl diimide (EDC2HCl) and N-hydroxysuccinimide (NHS). It is expected to obtain silk fibroin fiber artificial vascular materials with excellent mechanical properties and good biocompatibility, which can provide reference for the development of silk fiber artificial blood vessels. The main contents of this thesis are as follows: 1. The surface of silk fibroin fiber artificial vascular material was treated by low temperature and low pressure plasma technology, and the effect of gas, time, power, vacuum on the treatment effect was investigated. 2Graft of high and low molecular weight regenerated fibroin onto the surface of fibroin fiber artificial vascular material, and the effects of molecular weight and concentration of protein on grafting effect were investigated. 3. Protein adsorption, hemolysis and cytotoxicity of the modified fibroin fiber artificial vascular materials were studied, and their biocompatibility was evaluated. The results and main conclusions are as follows: (1) compared with the two kinds of plasma, He plasma treatment has the least influence on the surface morphology, structure and mechanical properties of silk fibroin fiber. Moreover, the hydrophilicity of the surface of the material can be improved significantly. The optimized process of He plasma treatment is as follows: time 180 s, power 70 wand, vacuum degree 50 Pa. (2) after high molecular weight regenerated fibroin was grafted, The tensile strength of fibroin fiber artificial vascular material was improved, but the elasticity and hydrophilicity of the material decreased. The tensile strength of fibroin fiber artificial vascular materials was also improved after grafting with low molecular weight regenerated silk fibroin. The grafted fibroin fiber had better homogeneity and stability, and the degree of elasticity and hydrophilicity decreased slightly. The optimized graft concentration was 1% high molecular weight regenerated silk fibroin solution and 5% low molecular weight regenerated silk fibroin solution. (3) High molecular weight regenerated silk fibroin was grafted. Fibroin fiber artificial vascular materials adsorb more plasma albumin. The results of hemolysis and cytotoxicity test showed that compared with low molecular weight regenerated fibroin, the graft of high molecular weight regenerated fibroin could significantly improve the blood and cytocompatibility of the materials. To sum up, the surface morphology of silk fibroin fiber artificial vascular material can be kept intact by He plasma treatment, and the mechanical property loss is small, and the hydrophilicity is improved obviously. Compared with the low molecular weight regenerated fibroin protein, grafted high molecular weight regenerated fibroin protein can obviously improve the biocompatibility of fibroin fiber artificial vascular materials, but there are some problems such as the decrease of material elasticity after grafting. The grafting process needs to be further improved.
【學(xué)位授予單位】：東華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08

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