本文選題：喉軟骨 + 骨髓　； 參考：《中國組織工程研究》2016年07期

【摘要】：背景:由于軟骨細胞缺乏再生能力,選擇合適的種子細胞是修復軟骨缺損需首要解決的問題。目的:探討同種異體和異種來源骨髓間充質干細胞成軟骨誘導后修復喉軟骨缺損的效果。方法:取第3代人骨髓間充質干細胞和兔骨髓間充質干細胞加入軟骨定向誘導液(含轉化生長因子β1和骨形態(tài)發(fā)生蛋白)進行成軟骨誘導,并滴加于聚乳酸-羥基乙酸共聚物支架上。取30只新西蘭大白兔隨機分為3組:空白對照組、人骨髓間充質干細胞修復組和兔骨髓間充質干細胞修復組,制備喉軟骨缺損動物模型,分別植入生理鹽水浸濕的聚乳酸-羥基乙酸共聚物支架,人骨髓間充質干細胞復合聚乳酸-羥基乙酸共聚物支架,兔骨髓間充質干細胞復合聚乳酸-羥基乙酸共聚物支架。術后4周和8周,免疫組化法檢測喉部組織Ⅱ型膠原的表達。結果與結論:各組動物均呼吸通暢、正常,未出現(xiàn)喘鳴等,進食和活動情況良好,未出現(xiàn)化膿或者感染現(xiàn)象。術后4周和8周,人骨髓間充質干細胞修復組和兔骨髓間充質干細胞修復組的Ⅱ型膠原陽性率均顯著高于空白對照組(P0.05)。人骨髓間充質干細胞修復組和兔骨髓間充質干細胞修復組間比較,差異無顯著性意義(P0.05)。結果表明同種異體和異種來源的骨髓間充質干細胞成軟骨誘導后進行兔喉軟骨缺損修復均可以獲得良好的效果,二者修復效果無顯著差異。
[Abstract]:Background: due to the lack of regeneration ability of chondrocytes, the choice of suitable seed cells is the most important problem in repairing cartilage defects. Aim: to investigate the effect of cartilage formation induced by allogeneic and xenogeneic bone marrow mesenchymal stem cells (MSCs) in repairing laryngeal cartilage defects. Methods: the third generation of human bone marrow mesenchymal stem cells and rabbit bone marrow mesenchymal stem cells were added into the chondrocyte directed inducer (TGF- 尾 1 and bone morphogenetic protein) to induce cartilage formation. It was added to the poly (lactic acid-glycolic acid) copolymer scaffold. Thirty New Zealand rabbits were randomly divided into three groups: blank control group, human bone marrow mesenchymal stem cell repair group and rabbit bone marrow mesenchymal stem cell repair group. The scaffolds of poly (lactic acid-glycolic acid) copolymers, human bone marrow mesenchymal stem cells (BMSCs) and poly (lactic acid-glycolic acid) copolymers, and rabbit bone marrow mesenchymal stem cells (BMSCs) were implanted respectively. The expression of type 鈪，

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