本文選題：硅基納米材料 + 表面增強(qiáng)拉曼散射��； 參考：《蘇州大學(xué)》2012年碩士論文

【摘要】：硅納米材料是一種重要的半導(dǎo)體納米材料，它具有優(yōu)異的電學(xué)和機(jī)械性能、良好的生物相容性、較大的比表面積、表面易修飾性，以及與傳統(tǒng)的硅科技的兼容性。因此，人們對(duì)發(fā)展功能化的硅納米材料有著濃厚的興趣，并已將其廣泛應(yīng)用于電子學(xué)和生物學(xué)領(lǐng)域。本文主要發(fā)展了基于硅基納米材料的表面增強(qiáng)拉曼散射（surface-enhanced Raman scattering, SERS）基質(zhì)，并用于DNA的高靈敏、特異性多元檢測(cè)；還研究了硅納米線在細(xì)胞層面的生物學(xué)效應(yīng)，主要研究內(nèi)容如下： 1、我們制備了一種基于硅基納米材料的表面增強(qiáng)拉曼散射基質(zhì)，即在硅片上沉積了一層銀納米粒子（silver nanoparticles-decorated silicon wafers,AgNPs@Si），并將其用于進(jìn)行高靈敏、特異性的DNA多元檢測(cè)。所制備AgNPs@Si具有極好的SERS信號(hào)重現(xiàn)性和較高的增強(qiáng)因子。我們的實(shí)驗(yàn)結(jié)果進(jìn)一步的證明了這種硅基SERS基底能夠有效地進(jìn)行DNA的高靈敏、特異性多元檢測(cè)。該研究對(duì)于發(fā)展具有良好重現(xiàn)性的SERS基質(zhì)，發(fā)展基于SERS的生物分子檢測(cè)方法具有積極的意義。 2、我們采用3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)細(xì)胞毒性測(cè)試、熒光成像和流式細(xì)胞術(shù)等經(jīng)典生物學(xué)方法，，系統(tǒng)研究了三種不同表面性質(zhì)的硅納米線對(duì)腫瘤細(xì)胞系和正常細(xì)胞株在不同條件下（如硅納米線濃度、共孵育時(shí)間等）的細(xì)胞毒性。研究結(jié)果發(fā)現(xiàn)：細(xì)胞毒性對(duì)硅納米線濃度和共孵育時(shí)間具有明顯依賴性：隨著硅納米線濃度的增加和共孵育時(shí)間的延長，細(xì)胞活性呈明顯下降趨勢(shì)；在相同條件下，腫瘤細(xì)胞株系較正常細(xì)胞株系具有更高的細(xì)胞活性；硅納米線表面性質(zhì)對(duì)細(xì)胞活性具有明顯的影響。在此基礎(chǔ)上,我們給出了硅納米線用于細(xì)胞層面生物應(yīng)用的安全閾值。
[Abstract]:Silicon nanomaterials are important semiconductor nanomaterials. They have excellent electrical and mechanical properties, good biocompatibility, large specific surface area, easy surface modification, and compatibility with traditional silicon technology. Therefore, there is a strong interest in the development of functionalized silicon nanomaterials, which have been widely used in electronics and biology. In this paper, the surface enhanced Raman scattering (SERS) matrix based on silicon-based nanomaterials has been developed and used for high sensitivity and specific multivariate detection of surface-enhanced, and the biological effects of silicon nanowires at the cellular level have also been studied. The main research contents are as follows: 1. We have prepared a surface-enhanced Raman scattering substrate based on silicon-based nanomaterials, that is, we deposited a silver nanoparticles-decorated silicon wafers AgNPs @ Si on silicon wafers and used it for high sensitivity. Specific DNA multivariate detection. The prepared AgNPs @ Si has excellent SERS signal reproducibility and high enhancement factor. Our experimental results further demonstrate that the Si-based SERS substrate can be used to detect DNA with high sensitivity and specificity. This study has positive significance for the development of SERS substrates with good reproducibility and the development of SERS-based biomolecules detection methods. 2. We used classical biological methods, such as 3- (4t5) -dimethylthiahiazo (-z-y1) -3-di-phenytetrazoliumromide (MTT) cytotoxicity test, fluorescence imaging and flow cytometry, etc. The cytotoxicity of three kinds of silicon nanowires with different surface properties to tumor cell lines and normal cell lines under different conditions such as the concentration of silicon nanowires and co-incubation time were studied. The results showed that the cytotoxicity was significantly dependent on the concentration and co-incubation time of silicon nanowires: with the increase of the concentration of silicon nanowires and the prolongation of co-incubation time, the cell activity decreased obviously. The cell activity of tumor cell line was higher than that of normal cell line, and the surface property of silicon nanowire had obvious influence on cell activity. On this basis, we give the safety threshold of silicon nanowires for biological applications at cell level.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

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